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Artigo
Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species
Germoplasm banking is an important tool for the preservation of genetic material from Neotropical primates in captivity, and from free living species, especially the endangered ones like Saimiri vanzolinii (Black-headed squirrel monkey), a primate with a low incidence area (870 km2 of floodplains)...
Autor principal: | Oliveira, Karol G |
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Outros Autores: | Santos, Regiane R, Le?o, Danuza L, Brito, Adriel B, Lima, Julianne S, Sampaio, Wla?sa V, Domingues, Sheyla Farhayldes Souza |
Grau: | Artigo |
Idioma: | eng |
Publicado em: |
Elsevier
2020
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Assuntos: | |
Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4023 |
Resumo: |
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Germoplasm banking is an important tool for the preservation of genetic material from Neotropical
primates in captivity, and from free living species, especially the endangered ones like Saimiri vanzolinii
(Black-headed squirrel monkey), a primate with a low incidence area (870 km2 of floodplains) in the
southern part of the Mamirau a Sustainable Development Reserve, Brazil. Therefore, in the present study
we aimed to develop a sperm cryopreservation protocol comparing sperm cooling in presence (T1) and
absence (T2) of egg yolk, and to test freezing protocols to preserve semen from captive (Saimiri collinsi),
and free-living (Saimiri vanzolinii, Saimiri cassiquiarensis and Saimiri macrodon) New World primates.
Cooling preserved sperm of S. collinsi in all evaluated microscopic parameters, except for sperm
motility. No differences were observed among the treatments, indicating that semen of this species
can be cooled without egg yolk. Freezing did not affect sperm quality of S. collinsi, except plasma
membrane integrity that was negatively affected. Generally, a good maintenance rate was observed
between cooling and thawing of semen for the four species, showing the positive translational
application of protocols from S. collinsi to the free-living species. Developed freezing protocol proved
to be useful for sperm cryopreservation of S. collinsi and in field conditions. |