Artigo

Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi)

The aim of this study was to evaluate ovarian tissue pre-treatment with 50??M Trolox followed by heterotopic transplantation in squirrel monkeys (Saimiri collinsi) and to assess tissue functionality via immunohistochemical analysis of the stroma and ovarian follicles. Five healthy and sexually matur...

ver descrição completa

Autor principal: Scalercio, Sarah Raphaella Rocha de Azevedo
Outros Autores: Amorim, Christiani A, Brito, Danielle C, Perc?rio, Sandro, Oskam, Irma C, Domingues, Sheyla Farhayldes Souza, Santos, Regiane R
Grau: Artigo
Idioma: eng
Publicado em: CSIRO Publishing 2020
Assuntos:
Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Ov?rio / anatomia & histologia
Transplante / veterin?ria
T?cnicas Reprodutivas / veterin?ria
Vasculariza??o
Acesso em linha: http://patua.iec.gov.br//handle/iec/4029
id ir-iec-4029
recordtype dspace
spelling ir-iec-40292020-02-14T11:57:54Z Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi) Scalercio, Sarah Raphaella Rocha de Azevedo Amorim, Christiani A Brito, Danielle C Perc?rio, Sandro Oskam, Irma C Domingues, Sheyla Farhayldes Souza Santos, Regiane R Primatas / anatomia & histologia Saimiri / anatomia & histologia Ov?rio / anatomia & histologia Transplante / veterin?ria T?cnicas Reprodutivas / veterin?ria Vasculariza??o The aim of this study was to evaluate ovarian tissue pre-treatment with 50??M Trolox followed by heterotopic transplantation in squirrel monkeys (Saimiri collinsi) and to assess tissue functionality via immunohistochemical analysis of the stroma and ovarian follicles. Five healthy and sexually mature squirrel monkey (Saimiri collinsi) females were used. Heterotopic autografting of fresh ovarian tissue with or without previous exposure to the antioxidant Trolox was performed and grafts were recovered for analysis 7 days later. Tissue vascularisation was confirmed by both macroscopic inspection and cluster of differentiation 31 (CD31) staining. Trolox prevented massive follicular activation and kept the percentages of morphologically normal follicles higher than in untreated grafts. Expression of anti-M?llerian hormone in developing follicles was observed only in controls and Trolox-treated grafts. Also, immunostaining for growth differentiation factor-9 was positive only in primordial follicles from controls and from Trolox-treated grafts. Although Trolox improved follicular quality and avoided apoptosis in stromal cells, ovarian tissue fibrosis was increased in Trolox-treated grafts, mainly due to an increase in collagen Type I synthesis. Capes 2020-02-13T17:01:12Z 2020-02-13T17:01:12Z 2016 Artigo SCALERCIO, Sarah Raphaella Rocha de Azevedo et al. Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi). Reproduction, Fertility and Development, v. 28, n. 11, p. 1854-1864, 2016. 1448-5990 http://patua.iec.gov.br//handle/iec/4029 10.1071/RD14454 eng Acesso Aberto application/pdf CSIRO Publishing
institution Instituto Evandro Chagas (IEC)
collection PATUA
language eng
topic Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Ov?rio / anatomia & histologia
Transplante / veterin?ria
T?cnicas Reprodutivas / veterin?ria
Vasculariza??o
spellingShingle Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Ov?rio / anatomia & histologia
Transplante / veterin?ria
T?cnicas Reprodutivas / veterin?ria
Vasculariza??o
Scalercio, Sarah Raphaella Rocha de Azevedo
Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi)
topic_facet Primatas / anatomia & histologia
Saimiri / anatomia & histologia
Ov?rio / anatomia & histologia
Transplante / veterin?ria
T?cnicas Reprodutivas / veterin?ria
Vasculariza??o
description The aim of this study was to evaluate ovarian tissue pre-treatment with 50??M Trolox followed by heterotopic transplantation in squirrel monkeys (Saimiri collinsi) and to assess tissue functionality via immunohistochemical analysis of the stroma and ovarian follicles. Five healthy and sexually mature squirrel monkey (Saimiri collinsi) females were used. Heterotopic autografting of fresh ovarian tissue with or without previous exposure to the antioxidant Trolox was performed and grafts were recovered for analysis 7 days later. Tissue vascularisation was confirmed by both macroscopic inspection and cluster of differentiation 31 (CD31) staining. Trolox prevented massive follicular activation and kept the percentages of morphologically normal follicles higher than in untreated grafts. Expression of anti-M?llerian hormone in developing follicles was observed only in controls and Trolox-treated grafts. Also, immunostaining for growth differentiation factor-9 was positive only in primordial follicles from controls and from Trolox-treated grafts. Although Trolox improved follicular quality and avoided apoptosis in stromal cells, ovarian tissue fibrosis was increased in Trolox-treated grafts, mainly due to an increase in collagen Type I synthesis.
format Artigo
author Scalercio, Sarah Raphaella Rocha de Azevedo
author2 Amorim, Christiani A
Brito, Danielle C
Perc?rio, Sandro
Oskam, Irma C
Domingues, Sheyla Farhayldes Souza
Santos, Regiane R
author2Str Amorim, Christiani A
Brito, Danielle C
Perc?rio, Sandro
Oskam, Irma C
Domingues, Sheyla Farhayldes Souza
Santos, Regiane R
title Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi)
title_short Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi)
title_full Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi)
title_fullStr Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi)
title_full_unstemmed Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi)
title_sort trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (saimiri collinsi)
publisher CSIRO Publishing
publishDate 2020
url http://patua.iec.gov.br//handle/iec/4029
_version_ 1717584482107654144
score 11.755432

Registros relacionados