Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986)

Coutinho, Carla Rafaela Monteiro; Siqueira, Jones Anderson Monteiro; Machado, Raiana Scerni; Bandeira, Renato da Silva; Ferreira, James Lima; Alves, Jainara Cristina dos Santos; Gabbay, Yvone Benchimol; Tavares, Fernando Neto

Artigo

Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986)

In the current investigation, fecal material was obtained during a community?based longitudinal study conducted from 1983 to 1986. This study consisted of 71 children aged newborn to 3 years. A total of 216 samples from three of these children were screened by real?time quantitative polymerase chain...

ver descrição completa

Autor principal:	Coutinho, Carla Rafaela Monteiro
Outros Autores:	Siqueira, Jones Anderson Monteiro, Machado, Raiana Scerni, Bandeira, Renato da Silva, Ferreira, James Lima, Alves, Jainara Cristina dos Santos, Gabbay, Yvone Benchimol, Tavares, Fernando Neto
Grau:	Artigo
Idioma:	eng
Publicado em:	Wiley 2020
Assuntos:	Enterovirus / isolamento & purifica??o Infec??es por Enterovirus / virologia Rea??o em Cadeia da Polimerase / m?todos
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4059

id	ir-iec-4059
recordtype	dspace
spelling	ir-iec-40592020-02-21T18:38:29Z Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986) Coutinho, Carla Rafaela Monteiro Siqueira, Jones Anderson Monteiro Machado, Raiana Scerni Bandeira, Renato da Silva Ferreira, James Lima Alves, Jainara Cristina dos Santos Gabbay, Yvone Benchimol Tavares, Fernando Neto Enterovirus / isolamento & purifica??o Infec??es por Enterovirus / virologia Rea??o em Cadeia da Polimerase / m?todos In the current investigation, fecal material was obtained during a community?based longitudinal study conducted from 1983 to 1986. This study consisted of 71 children aged newborn to 3 years. A total of 216 samples from three of these children were screened by real?time quantitative polymerase chain reaction (RT?qPCR) for the presence of enteroviruses, and positive samples were serotyped by VP1 and VP3 sequencing of the viral genome. Of these, 12 (5.6%) came from symptomatic cases, and the remaining asymptomatic cases were collected fortnightly during the 3 years of study. A positivity of 63.4% (137/216) was obtained by RT?qPCR, with 58.3% (7/12) in relation to the symptomatic group and 63.7% (130/204) in relation to the asymptomatic group. The 137 positive samples were inoculated into the RD, HEp2C, and L20B cell lines, and the cytopathic effect was observed in 37.2% (51/137) samples. It was also possible to identify 40.9% (56/137), between isolated (n?=?46) and nonisolated (n?=?10). Enterovirus serotype diversity (n?=?25) was identified in this study, with the predominant species being B (80.3%), followed by C (16.1%) and A (3.6%). Cases of reinfection by different serotypes were also observed in the three children studied. Analyses involving different age groups of these minors confirmed that the most affected age was between 12 to 24 months, with a prevalence of 77.6% (52/67). The enterovirus (EV) circulated in the 3 years of research, showed peaks in some months, without defined seasonality. This study demonstrated a high circulation and serotype diversity of EV in fecal samples, collected over 30 years ago. This endorsed the evaluation of important points of the epidemiology of these viruses, such as the presence of coinfection and reinfection of the same individual by different circulating serotypes. Understanding the frequency and duration of EV infections is important in determining their association with persistent diarrhea. 2020-02-21T18:22:38Z 2020-02-21T18:22:38Z 2020 Artigo COUTINHO, Carla Rafaela Monteiro et al. Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986). Journal of Medical Virology, v. xx, n.xx, p.xx, 2020. 1096-9071 http://patua.iec.gov.br//handle/iec/4059 10.1002/jmv.25656 eng Acesso Embargado application/pdf Wiley
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Enterovirus / isolamento & purifica??o Infec??es por Enterovirus / virologia Rea??o em Cadeia da Polimerase / m?todos
spellingShingle	Enterovirus / isolamento & purifica??o Infec??es por Enterovirus / virologia Rea??o em Cadeia da Polimerase / m?todos Coutinho, Carla Rafaela Monteiro Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986)
topic_facet	Enterovirus / isolamento & purifica??o Infec??es por Enterovirus / virologia Rea??o em Cadeia da Polimerase / m?todos
description	In the current investigation, fecal material was obtained during a community?based longitudinal study conducted from 1983 to 1986. This study consisted of 71 children aged newborn to 3 years. A total of 216 samples from three of these children were screened by real?time quantitative polymerase chain reaction (RT?qPCR) for the presence of enteroviruses, and positive samples were serotyped by VP1 and VP3 sequencing of the viral genome. Of these, 12 (5.6%) came from symptomatic cases, and the remaining asymptomatic cases were collected fortnightly during the 3 years of study. A positivity of 63.4% (137/216) was obtained by RT?qPCR, with 58.3% (7/12) in relation to the symptomatic group and 63.7% (130/204) in relation to the asymptomatic group. The 137 positive samples were inoculated into the RD, HEp2C, and L20B cell lines, and the cytopathic effect was observed in 37.2% (51/137) samples. It was also possible to identify 40.9% (56/137), between isolated (n?=?46) and nonisolated (n?=?10). Enterovirus serotype diversity (n?=?25) was identified in this study, with the predominant species being B (80.3%), followed by C (16.1%) and A (3.6%). Cases of reinfection by different serotypes were also observed in the three children studied. Analyses involving different age groups of these minors confirmed that the most affected age was between 12 to 24 months, with a prevalence of 77.6% (52/67). The enterovirus (EV) circulated in the 3 years of research, showed peaks in some months, without defined seasonality. This study demonstrated a high circulation and serotype diversity of EV in fecal samples, collected over 30 years ago. This endorsed the evaluation of important points of the epidemiology of these viruses, such as the presence of coinfection and reinfection of the same individual by different circulating serotypes. Understanding the frequency and duration of EV infections is important in determining their association with persistent diarrhea.
format	Artigo
author	Coutinho, Carla Rafaela Monteiro
author2	Siqueira, Jones Anderson Monteiro Machado, Raiana Scerni Bandeira, Renato da Silva Ferreira, James Lima Alves, Jainara Cristina dos Santos Gabbay, Yvone Benchimol Tavares, Fernando Neto
author2Str	Siqueira, Jones Anderson Monteiro Machado, Raiana Scerni Bandeira, Renato da Silva Ferreira, James Lima Alves, Jainara Cristina dos Santos Gabbay, Yvone Benchimol Tavares, Fernando Neto
title	Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986)
title_short	Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986)
title_full	Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986)
title_fullStr	Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986)
title_full_unstemmed	Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986)
title_sort	enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of belem city, amazon region, brazil, during the first 3 years of life (1983-1986)
publisher	Wiley
publishDate	2020
url	http://patua.iec.gov.br//handle/iec/4059
_version_	1717584380466036736
score	11.755432

Enterovirus detection and serotyping of fecal material collected from three children living on the outskirts of Belem city, Amazon region, Brazil, during the first 3 years of life (1983-1986)

Registros relacionados