Clinical and immunological features of human Leishmania (L.) infantum-Infection, novel insights Honduras, Central America
Leishmania (Leishmania) infantum is the etiological agent of both American visceral leishmaniasis (AVL) and non-ulcerated cutaneous leishmaniasis (NUCL) in Honduras. Although AVL is the most severe clinical form of infection, recent studies have shown that human immune response to parasite infect...
| Autor principal: | Sosa-Ochoa, Wilfredo |
|---|---|
| Outros Autores: | Z?niga, Concepci?n, Chaves, Luis Fernando, Flores, Gabriela Venicia Ara?jo, Pacheco, Carmen Maria Sandoval, Matta, Vania L?cia Ribeiro da, Corbett, Carlos Eduardo Pereira, Silveira, Fernando Tobias, Laurenti, Marcia Dalastra |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
MDPI
2020
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4118 |
| id |
ir-iec-4118 |
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dspace |
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ir-iec-41182020-07-20T14:40:51Z Clinical and immunological features of human Leishmania (L.) infantum-Infection, novel insights Honduras, Central America Sosa-Ochoa, Wilfredo Z?niga, Concepci?n Chaves, Luis Fernando Flores, Gabriela Venicia Ara?jo Pacheco, Carmen Maria Sandoval Matta, Vania L?cia Ribeiro da Corbett, Carlos Eduardo Pereira Silveira, Fernando Tobias Laurenti, Marcia Dalastra Leishmaniose Visceral / parasitologia Leishmania infantum / parasitologia Estudos Transversais Perfil de Sa?de Honduras (HON) Leishmania (Leishmania) infantum is the etiological agent of both American visceral leishmaniasis (AVL) and non-ulcerated cutaneous leishmaniasis (NUCL) in Honduras. Although AVL is the most severe clinical form of infection, recent studies have shown that human immune response to parasite infection can result in a clinical-immunological spectrum. The overall prevalence rate of infection and clinical-immunological profiles of the L. (L.) infantum infection in Amapala municipality, South Honduras was determined. We examined 576 individuals with diagnosis based on combined ELISA (IgG/IgM) and DTH assays. We also used genus-specific kDNA PCR and Hsp70 PCR-RFLP for NUCL cases. Clinical evaluation found 82% asymptomatic and 18% symptomatic individuals. All symptomatic cases (n = 104) showing NUCL were positive for parasites. We identified L. (L.) infantum species in 100% of the skin lesion scrapings and in 90% of the blood samples from NUCL cases studied. A total of 320 asymptomatic individuals were exposed (ELISA+ and/or DTH+), providing an overall L. (L.) infantum prevalence of 73.6%. Clinical, parasitological, and immunological evaluations suggest seven infection profiles, three asymptomatic and four symptomatic. This represents the first report on clinical and immunological features of human L. (L.) infantum-infection in Amapala municipality, Honduras. S?o Paulo Research Foundation #2016/50315-0 and Scientific Research Office of the UNAH (DICU-UNAH), Honduras #02-2015. CNPQ; CAPES 2020-07-20T14:05:12Z 2020-07-20T14:05:12Z 2020 Artigo SOSA-OCHOA, Wilfredo et al. Clinical and immunological features of human Leishmania (L.) infantum-Infection, novel insights Honduras, Central America. Pathogens, v. 9, n. 7, p. 1-15, 2020. 2076-0817 http://patua.iec.gov.br//handle/iec/4118 10.3390/pathogens9070554 eng Acesso Aberto application/pdf MDPI |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Leishmaniose Visceral / parasitologia Leishmania infantum / parasitologia Estudos Transversais Perfil de Sa?de Honduras (HON) |
| spellingShingle |
Leishmaniose Visceral / parasitologia Leishmania infantum / parasitologia Estudos Transversais Perfil de Sa?de Honduras (HON) Sosa-Ochoa, Wilfredo Clinical and immunological features of human Leishmania (L.) infantum-Infection, novel insights Honduras, Central America |
| topic_facet |
Leishmaniose Visceral / parasitologia Leishmania infantum / parasitologia Estudos Transversais Perfil de Sa?de Honduras (HON) |
| description |
Leishmania (Leishmania) infantum is the etiological agent of both American visceral
leishmaniasis (AVL) and non-ulcerated cutaneous leishmaniasis (NUCL) in Honduras. Although AVL
is the most severe clinical form of infection, recent studies have shown that human immune response
to parasite infection can result in a clinical-immunological spectrum. The overall prevalence rate of
infection and clinical-immunological profiles of the L. (L.) infantum infection in Amapala municipality,
South Honduras was determined. We examined 576 individuals with diagnosis based on combined
ELISA (IgG/IgM) and DTH assays. We also used genus-specific kDNA PCR and Hsp70 PCR-RFLP
for NUCL cases. Clinical evaluation found 82% asymptomatic and 18% symptomatic individuals.
All symptomatic cases (n = 104) showing NUCL were positive for parasites. We identified L. (L.)
infantum species in 100% of the skin lesion scrapings and in 90% of the blood samples from NUCL cases
studied. A total of 320 asymptomatic individuals were exposed (ELISA+ and/or DTH+), providing an
overall L. (L.) infantum prevalence of 73.6%. Clinical, parasitological, and immunological evaluations
suggest seven infection profiles, three asymptomatic and four symptomatic. This represents the
first report on clinical and immunological features of human L. (L.) infantum-infection in Amapala
municipality, Honduras. |
| format |
Artigo |
| author |
Sosa-Ochoa, Wilfredo |
| author2 |
Z?niga, Concepci?n Chaves, Luis Fernando Flores, Gabriela Venicia Ara?jo Pacheco, Carmen Maria Sandoval Matta, Vania L?cia Ribeiro da Corbett, Carlos Eduardo Pereira Silveira, Fernando Tobias Laurenti, Marcia Dalastra |
| author2Str |
Z?niga, Concepci?n Chaves, Luis Fernando Flores, Gabriela Venicia Ara?jo Pacheco, Carmen Maria Sandoval Matta, Vania L?cia Ribeiro da Corbett, Carlos Eduardo Pereira Silveira, Fernando Tobias Laurenti, Marcia Dalastra |
| title |
Clinical and immunological features of human Leishmania (L.) infantum-Infection, novel insights Honduras, Central America |
| title_short |
Clinical and immunological features of human Leishmania (L.) infantum-Infection, novel insights Honduras, Central America |
| title_full |
Clinical and immunological features of human Leishmania (L.) infantum-Infection, novel insights Honduras, Central America |
| title_fullStr |
Clinical and immunological features of human Leishmania (L.) infantum-Infection, novel insights Honduras, Central America |
| title_full_unstemmed |
Clinical and immunological features of human Leishmania (L.) infantum-Infection, novel insights Honduras, Central America |
| title_sort |
clinical and immunological features of human leishmania (l.) infantum-infection, novel insights honduras, central america |
| publisher |
MDPI |
| publishDate |
2020 |
| url |
http://patua.iec.gov.br//handle/iec/4118 |
| _version_ |
1717584454890815488 |
| score |
11.687526 |