Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region

Cruz, Ana Cec?lia Ribeiro; Nunes Neto, Joaquim Pinto; Silva, Sandro Patroca da; Silva, Eliana Vieira Pinto da; Pereira, Glennda Juscely Galv?o; Santos, Maissa Maia; Monteiro, Hamilton Ant?nio de Oliveira; Santos, Flavia Barreto dos; Guimar?es, Ricardo Jos? de Paula Souza e; Arag?o, Carine Fortes; Martins, L?via Car?cio

Artigo

Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region

Chikungunya virus (CHIKV) was first reported in Brazil in 2014 and, after it spread countrywide, an outbreak of febrile illness with reports of arthralgia happened in the municipality of Xinguara, Par?, Brazil in 2017, indicating the virus? circulation. Here, we aimed to investigate CHIKV in mosquit...

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Autor principal:	Cruz, Ana Cec?lia Ribeiro
Outros Autores:	Nunes Neto, Joaquim Pinto, Silva, Sandro Patroca da, Silva, Eliana Vieira Pinto da, Pereira, Glennda Juscely Galv?o, Santos, Maissa Maia, Monteiro, Hamilton Ant?nio de Oliveira, Santos, Flavia Barreto dos, Guimar?es, Ricardo Jos? de Paula Souza e, Arag?o, Carine Fortes, Martins, L?via Car?cio
Grau:	Artigo
Idioma:	eng
Publicado em:	MDPI 2020
Assuntos:	Aedes / anatomia & histologia Culex / virologia V?rus Chikungunya / patogenicidade Mosquitos Vetores / virologia Genoma Viral Gen?tipo da ?frica do Centro-Leste-Sul-Africano Gen?tipo Vigil?ncia Entomol?gica Xinguara (PA)
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4144

id	ir-iec-4144
recordtype	dspace
spelling	ir-iec-41442020-08-17T12:51:30Z Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region Cruz, Ana Cec?lia Ribeiro Nunes Neto, Joaquim Pinto Silva, Sandro Patroca da Silva, Eliana Vieira Pinto da Pereira, Glennda Juscely Galv?o Santos, Maissa Maia Monteiro, Hamilton Ant?nio de Oliveira Santos, Flavia Barreto dos Guimar?es, Ricardo Jos? de Paula Souza e Arag?o, Carine Fortes Martins, L?via Car?cio Aedes / anatomia & histologia Culex / virologia V?rus Chikungunya / patogenicidade Mosquitos Vetores / virologia Genoma Viral Gen?tipo da ?frica do Centro-Leste-Sul-Africano Gen?tipo Vigil?ncia Entomol?gica Xinguara (PA) Chikungunya virus (CHIKV) was first reported in Brazil in 2014 and, after it spread countrywide, an outbreak of febrile illness with reports of arthralgia happened in the municipality of Xinguara, Par?, Brazil in 2017, indicating the virus? circulation. Here, we aimed to investigate CHIKV in mosquito vectors collected during an active surveillance of virus isolation in cell culture by using molecular detection and viral genome sequencing. A total of 492 Aedes, Culex and Mansonia mosquitoes were collected and separated in 36 pools according to the species and sex, and 22.2% (8/36) were positive. CHIKV was indentified in pools of Ae. aegypti females (n = 5), an Ae. aegypti male (n = 1) and in Culex quinquefasciatus females (n = 2). However, as the mosquitoes? whole bodies were macerated and used for detection, one cannot suggest the role of the latter in the viral transmission. Despite this, vector competence studies must be carried out in the different species to investigate long-term adaptations. Viral genome sequencing has characterized the East-Central-South-African (ECSA) genotype in all positive pools analyzed, corroborating previous reports for the Amazon region. Instituto Evandro Chagas/Minist?rio da Sa?de and to Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) grant number 302462/2018-0 2020-08-17T12:20:34Z 2020-08-17T12:20:34Z 2020 Artigo CRUZ, Ana Cec?lia Ribeiro et al. Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region. Viruses, v. 12, n. 8, p. 1- 11, Aug. 2020. 1999-4915 http://patua.iec.gov.br//handle/iec/4144 10.3390/v12080853 eng Acesso Aberto application/pdf MDPI
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Aedes / anatomia & histologia Culex / virologia V?rus Chikungunya / patogenicidade Mosquitos Vetores / virologia Genoma Viral Gen?tipo da ?frica do Centro-Leste-Sul-Africano Gen?tipo Vigil?ncia Entomol?gica Xinguara (PA)
spellingShingle	Aedes / anatomia & histologia Culex / virologia V?rus Chikungunya / patogenicidade Mosquitos Vetores / virologia Genoma Viral Gen?tipo da ?frica do Centro-Leste-Sul-Africano Gen?tipo Vigil?ncia Entomol?gica Xinguara (PA) Cruz, Ana Cec?lia Ribeiro Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region
topic_facet	Aedes / anatomia & histologia Culex / virologia V?rus Chikungunya / patogenicidade Mosquitos Vetores / virologia Genoma Viral Gen?tipo da ?frica do Centro-Leste-Sul-Africano Gen?tipo Vigil?ncia Entomol?gica Xinguara (PA)
description	Chikungunya virus (CHIKV) was first reported in Brazil in 2014 and, after it spread countrywide, an outbreak of febrile illness with reports of arthralgia happened in the municipality of Xinguara, Par?, Brazil in 2017, indicating the virus? circulation. Here, we aimed to investigate CHIKV in mosquito vectors collected during an active surveillance of virus isolation in cell culture by using molecular detection and viral genome sequencing. A total of 492 Aedes, Culex and Mansonia mosquitoes were collected and separated in 36 pools according to the species and sex, and 22.2% (8/36) were positive. CHIKV was indentified in pools of Ae. aegypti females (n = 5), an Ae. aegypti male (n = 1) and in Culex quinquefasciatus females (n = 2). However, as the mosquitoes? whole bodies were macerated and used for detection, one cannot suggest the role of the latter in the viral transmission. Despite this, vector competence studies must be carried out in the different species to investigate long-term adaptations. Viral genome sequencing has characterized the East-Central-South-African (ECSA) genotype in all positive pools analyzed, corroborating previous reports for the Amazon region.
format	Artigo
author	Cruz, Ana Cec?lia Ribeiro
author2	Nunes Neto, Joaquim Pinto Silva, Sandro Patroca da Silva, Eliana Vieira Pinto da Pereira, Glennda Juscely Galv?o Santos, Maissa Maia Monteiro, Hamilton Ant?nio de Oliveira Santos, Flavia Barreto dos Guimar?es, Ricardo Jos? de Paula Souza e Arag?o, Carine Fortes Martins, L?via Car?cio
author2Str	Nunes Neto, Joaquim Pinto Silva, Sandro Patroca da Silva, Eliana Vieira Pinto da Pereira, Glennda Juscely Galv?o Santos, Maissa Maia Monteiro, Hamilton Ant?nio de Oliveira Santos, Flavia Barreto dos Guimar?es, Ricardo Jos? de Paula Souza e Arag?o, Carine Fortes Martins, L?via Car?cio
title	Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region
title_short	Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region
title_full	Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region
title_fullStr	Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region
title_full_unstemmed	Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region
title_sort	chikungunya virus detection in aedes aegypti and culex quinquefasciatus during an outbreak in the amazon region
publisher	MDPI
publishDate	2020
url	http://patua.iec.gov.br//handle/iec/4144
_version_	1717584429081165824
score	11.755432

Chikungunya virus detection in Aedes aegypti and Culex quinquefasciatus during an outbreak in the Amazon Region

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