Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory
Zika and Dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. Due to Brazil?s wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by National Ref...
| Autor principal: | Henriques, Daniele Freitas |
|---|---|
| Outros Autores: | Nunes, Juliana A. L, Anjos, Maura V, Melo, Juliana M, Ros?rio, Wallace O, Azevedo, Raimunda do Socorro da Silva, Chiang, Jannifer Oliveira, Martins, Livia Car?cio, Santos, Flavia B. dos, Casseb, Livia Medeiros Neves, Vasconcelos, Pedro Fernando da Costa, Rodrigues, Sueli Guerreiro |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Elsevier
2020
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4182 |
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ir-iec-4182 |
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| recordtype |
dspace |
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ir-iec-41822020-10-13T18:02:34Z Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory Henriques, Daniele Freitas Nunes, Juliana A. L Anjos, Maura V Melo, Juliana M Ros?rio, Wallace O Azevedo, Raimunda do Socorro da Silva Chiang, Jannifer Oliveira Martins, Livia Car?cio Santos, Flavia B. dos Casseb, Livia Medeiros Neves Vasconcelos, Pedro Fernando da Costa Rodrigues, Sueli Guerreiro Flavivirus / isolamento & purifica??o Infec??es por Flavivirus Dengue Zika Virus Febre Amarela Ensaio de Imunoadsor??o Enzim?tica Imunoglobulina M Testes Laboratoriais Vigil?ncia de Produtos Comercializados Zika and Dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. Due to Brazil?s wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by National Reference Laboratory for Arboviruses in Brazil and evaluate commercial tests available. We evaluated in house IgM ELISAs for the individual detection of anti-ZIKV, -DENV, and ?YFV IgM, against a panel of samples positive for dengue, zika, yellow fever, Rocio, Ilheus, Saint Louis encephalitis, West Nile and chikungunya. We also evaluated two commercial kits for dengue and zika IgM detection recommended by the Brazilian Ministry of Health in 2015. The sensitivity and specificity for the in house ZIKV IgM ELISA was 60.0 % and 88.6 % and for the in house DENV IgM ELISA was 100 % and 82.2 %, respectively. The in house YFV IgM ELISA presented 100 % for both sensitivity and specificity. The Novagnost Zika Virus IgM test presented a sensitivity of 47.3 % and specificity of 85.3 % and the Serion ELISA classic Dengue Virus IgM, 92.8 % and 58.9 %, respectively. Overall, both in house ELISAs for ZIKV and DENV adapted and evaluated here, presented better performances than the commercial kits tested. Evandro Chagas Institute/Ministry of Health (IEC/MS) from the Brazilian Government. 2020-10-13T17:44:22Z 2020-10-13T17:44:22Z 2020 Artigo HENRIQUES, Daniele Freitas et al. Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory. Journal of Virological Methods, v. 286, n. 113976, p. xx, Dec. 2020. 0166-0934 http://patua.iec.gov.br//handle/iec/4182 10.1016/j.jviromet.2020.113976 eng Acesso Aberto application/pdf Elsevier |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Flavivirus / isolamento & purifica??o Infec??es por Flavivirus Dengue Zika Virus Febre Amarela Ensaio de Imunoadsor??o Enzim?tica Imunoglobulina M Testes Laboratoriais Vigil?ncia de Produtos Comercializados |
| spellingShingle |
Flavivirus / isolamento & purifica??o Infec??es por Flavivirus Dengue Zika Virus Febre Amarela Ensaio de Imunoadsor??o Enzim?tica Imunoglobulina M Testes Laboratoriais Vigil?ncia de Produtos Comercializados Henriques, Daniele Freitas Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| topic_facet |
Flavivirus / isolamento & purifica??o Infec??es por Flavivirus Dengue Zika Virus Febre Amarela Ensaio de Imunoadsor??o Enzim?tica Imunoglobulina M Testes Laboratoriais Vigil?ncia de Produtos Comercializados |
| description |
Zika and Dengue viruses present considerable immunological cross-reactivity, resulting in a troublesome serodiagnosis due to occurrence of false positive results. Due to Brazil?s wide variety of circulating flaviviruses we aimed to access the use of in house serological tests adapted by National Reference Laboratory for Arboviruses in Brazil and evaluate commercial tests available. We evaluated in house IgM ELISAs for the individual detection of anti-ZIKV, -DENV, and ?YFV IgM, against a panel of samples positive for dengue, zika, yellow fever, Rocio, Ilheus, Saint Louis encephalitis, West Nile and chikungunya. We also evaluated two commercial kits for dengue and zika IgM detection recommended by the Brazilian Ministry of Health in 2015. The sensitivity and specificity for the in house ZIKV IgM ELISA was 60.0 % and 88.6 % and for the in house DENV IgM ELISA was 100 % and 82.2 %, respectively. The in house YFV IgM ELISA presented 100 % for both sensitivity and specificity. The Novagnost Zika Virus IgM test presented a sensitivity of 47.3 % and specificity of 85.3 % and the Serion ELISA classic Dengue Virus IgM, 92.8 % and 58.9 %, respectively. Overall, both in house ELISAs for ZIKV and DENV adapted and evaluated here, presented better performances than the commercial kits tested. |
| format |
Artigo |
| author |
Henriques, Daniele Freitas |
| author2 |
Nunes, Juliana A. L Anjos, Maura V Melo, Juliana M Ros?rio, Wallace O Azevedo, Raimunda do Socorro da Silva Chiang, Jannifer Oliveira Martins, Livia Car?cio Santos, Flavia B. dos Casseb, Livia Medeiros Neves Vasconcelos, Pedro Fernando da Costa Rodrigues, Sueli Guerreiro |
| author2Str |
Nunes, Juliana A. L Anjos, Maura V Melo, Juliana M Ros?rio, Wallace O Azevedo, Raimunda do Socorro da Silva Chiang, Jannifer Oliveira Martins, Livia Car?cio Santos, Flavia B. dos Casseb, Livia Medeiros Neves Vasconcelos, Pedro Fernando da Costa Rodrigues, Sueli Guerreiro |
| title |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title_short |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title_full |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title_fullStr |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title_full_unstemmed |
Evaluation of immunoglobulin M-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a National Reference Laboratory |
| title_sort |
evaluation of immunoglobulin m-specific capture enzyme-linked immunosorbent assays and commercial tests for flaviviruses diagnosis by a national reference laboratory |
| publisher |
Elsevier |
| publishDate |
2020 |
| url |
http://patua.iec.gov.br//handle/iec/4182 |
| _version_ |
1717584429753303040 |
| score |
11.755432 |