Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil

Silva, Aniel de Sarom Negr?o; Lima, Clayton Pereira Silva de; Barata, Rafael Ribeiro; Silva, Pedro Victor Reis da; Monteiro, Patricia Danin Jord?o; Lamar?o, Let?cia; Burbano, Rommel M?rio Rodr?gues; Nunes, M?rcio Roberto Teixeira; Lima, Danielle Lima de

Artigo

Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil

Human pegivirus (HPgV)?formerly known as GBV-C?is a member of the Flaviviridae family and belongs to the species Pegivirus C. It is a non-pathogenic virus and is transmitted among humans mainly through the exposure to contaminated blood and is often associated with human immunodeficiency virus (HIV)...

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Autor principal:	Silva, Aniel de Sarom Negr?o
Outros Autores:	Lima, Clayton Pereira Silva de, Barata, Rafael Ribeiro, Silva, Pedro Victor Reis da, Monteiro, Patricia Danin Jord?o, Lamar?o, Let?cia, Burbano, Rommel M?rio Rodr?gues, Nunes, M?rcio Roberto Teixeira, Lima, Danielle Lima de
Grau:	Artigo
Idioma:	eng
Publicado em:	BMC 2020
Assuntos:	Sangue / virologia HIV / gen?tica Infec??es por V?rus de RNA / sangue Coinfec??o / sangue Coinfec??o / virologia Carga Viral Preval?ncia Doadores de Sangue
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4188

id	ir-iec-4188
recordtype	dspace
spelling	ir-iec-41882020-10-26T13:53:19Z Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil Silva, Aniel de Sarom Negr?o Lima, Clayton Pereira Silva de Barata, Rafael Ribeiro Silva, Pedro Victor Reis da Monteiro, Patricia Danin Jord?o Lamar?o, Let?cia Burbano, Rommel M?rio Rodr?gues Nunes, M?rcio Roberto Teixeira Lima, Danielle Lima de Sangue / virologia HIV / gen?tica Infec??es por V?rus de RNA / sangue Coinfec??o / sangue Coinfec??o / virologia Carga Viral Preval?ncia Doadores de Sangue Human pegivirus (HPgV)?formerly known as GBV-C?is a member of the Flaviviridae family and belongs to the species Pegivirus C. It is a non-pathogenic virus and is transmitted among humans mainly through the exposure to contaminated blood and is often associated with human immunodeficiency virus (HIV) infection, among other viruses. This study aimed to determine the prevalence of HPgV viremia, its association with HIV and clinical epidemiological factors, as well as the full-length sequencing and genome characterization of HPgV recovered from blood donors of the HEMOPA Foundation in Bel?m-PA-Brazil. Methods: Plasma samples were obtained from 459 donors, tested for the presence of HPgV RNA by the RT-qPCR. From these, a total of 26 RT-qPCR positive samples were submitted to the NGS sequencing approach in order to obtain the full genome. Genome characterization and phylogenetic analysis were conducted. Results: The prevalence of HPgV was 12.42%. We observed the highest prevalences among donors aged between 18 and 30 years old (16.5%), with brown skin color (13.2%) and men (15.8%). The newly diagnosed HIV-1 prevalence was 26.67%. The HPgV genotype 2 (2a and 2b) was identified. No data on viral load value was found to corroborate the protective effect of HPgV on HIV evolution. Conclusions: This study provided information regarding the HPgV infection among blood donors from HEMOPA Foundation. Furthermore, we genetically characterized the HPgV circulating strains and described by the first time nearly complete genomes of genotype 2 in Brazilian Amazon. HEMOPA foundation, Evandro Chagas Institute, Foundation for Scientific and Technological Development in Health (FIOTEC; Project PRES-012-FIO-16) and funded in part by the research productivity project CNPq (302584/2015?3) 2020-10-26T13:01:14Z 2020-10-26T13:01:14Z 2020 Artigo SILVA, Aniel de Sarom Negr?o et al. Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil. Virology Journal, v. 17, n. 1 p. 1-8, Dec. 2020. 1743-422X http://patua.iec.gov.br//handle/iec/4188 10.1186/s12985-020-01427-6 eng Acesso Aberto application/pdf BMC
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Sangue / virologia HIV / gen?tica Infec??es por V?rus de RNA / sangue Coinfec??o / sangue Coinfec??o / virologia Carga Viral Preval?ncia Doadores de Sangue
spellingShingle	Sangue / virologia HIV / gen?tica Infec??es por V?rus de RNA / sangue Coinfec??o / sangue Coinfec??o / virologia Carga Viral Preval?ncia Doadores de Sangue Silva, Aniel de Sarom Negr?o Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil
topic_facet	Sangue / virologia HIV / gen?tica Infec??es por V?rus de RNA / sangue Coinfec??o / sangue Coinfec??o / virologia Carga Viral Preval?ncia Doadores de Sangue
description	Human pegivirus (HPgV)?formerly known as GBV-C?is a member of the Flaviviridae family and belongs to the species Pegivirus C. It is a non-pathogenic virus and is transmitted among humans mainly through the exposure to contaminated blood and is often associated with human immunodeficiency virus (HIV) infection, among other viruses. This study aimed to determine the prevalence of HPgV viremia, its association with HIV and clinical epidemiological factors, as well as the full-length sequencing and genome characterization of HPgV recovered from blood donors of the HEMOPA Foundation in Bel?m-PA-Brazil. Methods: Plasma samples were obtained from 459 donors, tested for the presence of HPgV RNA by the RT-qPCR. From these, a total of 26 RT-qPCR positive samples were submitted to the NGS sequencing approach in order to obtain the full genome. Genome characterization and phylogenetic analysis were conducted. Results: The prevalence of HPgV was 12.42%. We observed the highest prevalences among donors aged between 18 and 30 years old (16.5%), with brown skin color (13.2%) and men (15.8%). The newly diagnosed HIV-1 prevalence was 26.67%. The HPgV genotype 2 (2a and 2b) was identified. No data on viral load value was found to corroborate the protective effect of HPgV on HIV evolution. Conclusions: This study provided information regarding the HPgV infection among blood donors from HEMOPA Foundation. Furthermore, we genetically characterized the HPgV circulating strains and described by the first time nearly complete genomes of genotype 2 in Brazilian Amazon.
format	Artigo
author	Silva, Aniel de Sarom Negr?o
author2	Lima, Clayton Pereira Silva de Barata, Rafael Ribeiro Silva, Pedro Victor Reis da Monteiro, Patricia Danin Jord?o Lamar?o, Let?cia Burbano, Rommel M?rio Rodr?gues Nunes, M?rcio Roberto Teixeira Lima, Danielle Lima de
author2Str	Lima, Clayton Pereira Silva de Barata, Rafael Ribeiro Silva, Pedro Victor Reis da Monteiro, Patricia Danin Jord?o Lamar?o, Let?cia Burbano, Rommel M?rio Rodr?gues Nunes, M?rcio Roberto Teixeira Lima, Danielle Lima de
title	Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil
title_short	Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil
title_full	Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil
title_fullStr	Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil
title_full_unstemmed	Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil
title_sort	human pegivirus (hpgv, gbv-c) rna in volunteer blood donors from a public hemotherapy service in northern brazil
publisher	BMC
publishDate	2020
url	http://patua.iec.gov.br//handle/iec/4188
_version_	1717584381486301184
score	11.653393

Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil

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