Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)

Silva, F?bio Silva da; Cruz, Ana Cec?lia Ribeiro; Medeiros, Daniele Barbosa de Almeida; Silva, Sandro Patroca da; Nunes, M?rcio Roberto Teixeira; Martins, L?via Car?cio; Chiang, Jannifer Oliveira; Lemos, Poliana da Silva; Cunha, Gabriel Muricy; Araujo, Renato Freitas de; Monteiro, Hamilton Ant?nio de Oliveira; Nunes Neto, Joaquim Pinto

Artigo

Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)

The genus Haemagogus (Diptera: Culicidae) comprises species of great epidemiological relevance, involved in transmission cycles of the Yellow fever virus and other arboviruses in South America. So far, only Haemagogus janthinomys has complete mitochondrial sequences available. Given the unavailabili...

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Autor principal:	Silva, F?bio Silva da
Outros Autores:	Cruz, Ana Cec?lia Ribeiro, Medeiros, Daniele Barbosa de Almeida, Silva, Sandro Patroca da, Nunes, M?rcio Roberto Teixeira, Martins, L?via Car?cio, Chiang, Jannifer Oliveira, Lemos, Poliana da Silva, Cunha, Gabriel Muricy, Araujo, Renato Freitas de, Monteiro, Hamilton Ant?nio de Oliveira, Nunes Neto, Joaquim Pinto
Grau:	Artigo
Idioma:	eng
Publicado em:	Nature Research 2020
Assuntos:	D?pteros / classifica??o Culicidae / classifica??o Mitoc?ndrias / gen?tica Filogenia Sequenciamento Completo do Genoma
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4189

id	ir-iec-4189
recordtype	dspace
spelling	ir-iec-41892020-10-26T14:26:29Z Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae) Silva, F?bio Silva da Cruz, Ana Cec?lia Ribeiro Medeiros, Daniele Barbosa de Almeida Silva, Sandro Patroca da Nunes, M?rcio Roberto Teixeira Martins, L?via Car?cio Chiang, Jannifer Oliveira Lemos, Poliana da Silva Cunha, Gabriel Muricy Araujo, Renato Freitas de Monteiro, Hamilton Ant?nio de Oliveira Nunes Neto, Joaquim Pinto D?pteros / classifica??o Culicidae / classifica??o Mitoc?ndrias / gen?tica Filogenia Sequenciamento Completo do Genoma The genus Haemagogus (Diptera: Culicidae) comprises species of great epidemiological relevance, involved in transmission cycles of the Yellow fever virus and other arboviruses in South America. So far, only Haemagogus janthinomys has complete mitochondrial sequences available. Given the unavailability of information related to aspects of the evolutionary biology and molecular taxonomy of this genus, we report here, the first sequencing of the mitogenomes of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis. The mitogenomes showed an average length of 15,038 bp, average AT content of 79.3%, positive AT-skews, negative GC-skews, and comprised 37 functional subunits (13 PCGs, 22 tRNA, and 02 rRNA). The PCGs showed ATN as start codon, TAA as stop codon, and signs of purifying selection. The tRNAs had the typical leaf clover structure, except tRNASer1. Phylogenetic analyzes of Bayesian inference and Maximum Likelihood, based on concatenated sequences from all 13 PCGs, produced identical topologies and strongly supported the monophyletic relationship between the Haemagogus and Conopostegus subgenera, and corroborated with the known taxonomic classification of the evaluated taxa, based on external morphological aspects. The information produced on the mitogenomes of the Haemagogus species evaluated here may be useful in carrying out future taxonomic and evolutionary studies of the genus. Evandro Chagas Institute (IEC/MS/SVS); Coordination for the Improvement of Higher Education Personnel (CAPES/1743623). 2020-10-26T14:07:57Z 2020-10-26T14:07:57Z 2020 Artigo SILVA, F?bio Silva da et al. Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae). Scientific Reports, v. 10, n. 1, p. 1-13, Dec. 2020. 2045-2322 http://patua.iec.gov.br//handle/iec/4189 10.1038/s41598-020-73790-x eng Acesso Aberto application/pdf Nature Research
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	D?pteros / classifica??o Culicidae / classifica??o Mitoc?ndrias / gen?tica Filogenia Sequenciamento Completo do Genoma
spellingShingle	D?pteros / classifica??o Culicidae / classifica??o Mitoc?ndrias / gen?tica Filogenia Sequenciamento Completo do Genoma Silva, F?bio Silva da Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)
topic_facet	D?pteros / classifica??o Culicidae / classifica??o Mitoc?ndrias / gen?tica Filogenia Sequenciamento Completo do Genoma
description	The genus Haemagogus (Diptera: Culicidae) comprises species of great epidemiological relevance, involved in transmission cycles of the Yellow fever virus and other arboviruses in South America. So far, only Haemagogus janthinomys has complete mitochondrial sequences available. Given the unavailability of information related to aspects of the evolutionary biology and molecular taxonomy of this genus, we report here, the first sequencing of the mitogenomes of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis. The mitogenomes showed an average length of 15,038 bp, average AT content of 79.3%, positive AT-skews, negative GC-skews, and comprised 37 functional subunits (13 PCGs, 22 tRNA, and 02 rRNA). The PCGs showed ATN as start codon, TAA as stop codon, and signs of purifying selection. The tRNAs had the typical leaf clover structure, except tRNASer1. Phylogenetic analyzes of Bayesian inference and Maximum Likelihood, based on concatenated sequences from all 13 PCGs, produced identical topologies and strongly supported the monophyletic relationship between the Haemagogus and Conopostegus subgenera, and corroborated with the known taxonomic classification of the evaluated taxa, based on external morphological aspects. The information produced on the mitogenomes of the Haemagogus species evaluated here may be useful in carrying out future taxonomic and evolutionary studies of the genus.
format	Artigo
author	Silva, F?bio Silva da
author2	Cruz, Ana Cec?lia Ribeiro Medeiros, Daniele Barbosa de Almeida Silva, Sandro Patroca da Nunes, M?rcio Roberto Teixeira Martins, L?via Car?cio Chiang, Jannifer Oliveira Lemos, Poliana da Silva Cunha, Gabriel Muricy Araujo, Renato Freitas de Monteiro, Hamilton Ant?nio de Oliveira Nunes Neto, Joaquim Pinto
author2Str	Cruz, Ana Cec?lia Ribeiro Medeiros, Daniele Barbosa de Almeida Silva, Sandro Patroca da Nunes, M?rcio Roberto Teixeira Martins, L?via Car?cio Chiang, Jannifer Oliveira Lemos, Poliana da Silva Cunha, Gabriel Muricy Araujo, Renato Freitas de Monteiro, Hamilton Ant?nio de Oliveira Nunes Neto, Joaquim Pinto
title	Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)
title_short	Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)
title_full	Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)
title_fullStr	Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)
title_full_unstemmed	Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)
title_sort	mitochondrial genome sequencing and phylogeny of haemagogus albomaculatus, haemagogus leucocelaenus, haemagogus spegazzinii, and haemagogus tropicalis (diptera: culicidae)
publisher	Nature Research
publishDate	2020
url	http://patua.iec.gov.br//handle/iec/4189
_version_	1717584538772701184
score	11.755432

Mitochondrial genome sequencing and phylogeny of Haemagogus albomaculatus, Haemagogus leucocelaenus, Haemagogus spegazzinii, and Haemagogus tropicalis (Diptera: Culicidae)

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