Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy
BACKGROUND Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of transmission cycles in endemic areas. OBJECTIVE To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and blood, brain, liver, ki...
| Autor principal: | Ferreira, Milene Silveira |
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| Outros Autores: | Bezerra Junior, Pedro Soares, Cerqueira, Val?ria Duarte, Rivero, Gabriela Riet Correa, Oliveira Junior, Carlos Alberto, Castro, Paulo Henrique Gomes, Silva, Gilmara Abreu da, Silva, Wellington Bandeira da, Imbeloni, Aline Amaral, Sousa, Jorge Rodrigues, Ara?jo, Ana Paula Sousa, Silva, Franko de Arruda e, Tesh, Robert B, Quaresma, Juarez Ant?nio Sim?es, Vasconcelos, Pedro Fernando da Costa |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
Instituto Oswaldo Cruz
2020
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| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4202 |
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ir-iec-4202 |
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ir-iec-42022020-11-23T17:34:15Z Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy Ferreira, Milene Silveira Bezerra Junior, Pedro Soares Cerqueira, Val?ria Duarte Rivero, Gabriela Riet Correa Oliveira Junior, Carlos Alberto Castro, Paulo Henrique Gomes Silva, Gilmara Abreu da Silva, Wellington Bandeira da Imbeloni, Aline Amaral Sousa, Jorge Rodrigues Ara?jo, Ana Paula Sousa Silva, Franko de Arruda e Tesh, Robert B Quaresma, Juarez Ant?nio Sim?es Vasconcelos, Pedro Fernando da Costa Febre Amarela / veterin?ria V?rus da Febre Amarela / patogenicidade Saimiri / anatomia & histologia BACKGROUND Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of transmission cycles in endemic areas. OBJECTIVE To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and blood, brain, liver, kidney, spleen, heart, lung, lymph node and stomach were collected at 1-7, 10, 20 and 30 days post-infection (dpi). METHODS Histopathological analysis and detection of the genome and viral antigens and neutralising antibodies were performed by RT-PCR, immunohistochemistry and neutralisation test, respectively. FINDINGS Only one animal died from the experimental infection. The genome and viral antigens were detected in all investigated organs (1-30 dpi) and the neutralising antibodies from seven to 30 dpi. The brain contained perivascular haemorrhage (6 dpi); in the liver, midzonal haemorrhage and lytic necrosis (6 dpi) were observed. The kidney had bleeding in the Bowman?s capsule and tubular necrosis (6 dpi). Pyknotic lymphocytes were observed in the spleen (1-20 dpi), the lung had haemorrhage (2-6 dpi), in the endocardium it contained nuclear pyknosis and necrosis (2-3 dpi) and the stomach contained blood in the lumen (6 dpi). MAIN FINDINGS Squirrel monkeys reliably reproduced the responses observed in human cases of yellow fever and, therefore, constitute an excellent experimental model for studies on the pathophysiology of the disease. 2020-11-23T17:18:18Z 2020-11-23T17:18:18Z 2020 Artigo FERREIRA, Milene Silveira et al. Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy. Mem?rias do Instituto Oswaldo Cruz, v. 115, e190501, Nov. 2020. 1678-8060 http://patua.iec.gov.br//handle/iec/4202 10.1590/0074-02760190501 eng Acesso Aberto application/pdf Instituto Oswaldo Cruz |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Febre Amarela / veterin?ria V?rus da Febre Amarela / patogenicidade Saimiri / anatomia & histologia |
| spellingShingle |
Febre Amarela / veterin?ria V?rus da Febre Amarela / patogenicidade Saimiri / anatomia & histologia Ferreira, Milene Silveira Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy |
| topic_facet |
Febre Amarela / veterin?ria V?rus da Febre Amarela / patogenicidade Saimiri / anatomia & histologia |
| description |
BACKGROUND Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of
transmission cycles in endemic areas.
OBJECTIVE To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and
blood, brain, liver, kidney, spleen, heart, lung, lymph node and stomach were collected at 1-7, 10, 20 and 30 days post-infection (dpi).
METHODS Histopathological analysis and detection of the genome and viral antigens and neutralising antibodies were performed
by RT-PCR, immunohistochemistry and neutralisation test, respectively.
FINDINGS Only one animal died from the experimental infection. The genome and viral antigens were detected in all investigated
organs (1-30 dpi) and the neutralising antibodies from seven to 30 dpi. The brain contained perivascular haemorrhage (6 dpi);
in the liver, midzonal haemorrhage and lytic necrosis (6 dpi) were observed. The kidney had bleeding in the Bowman?s capsule
and tubular necrosis (6 dpi). Pyknotic lymphocytes were observed in the spleen (1-20 dpi), the lung had haemorrhage (2-6 dpi),
in the endocardium it contained nuclear pyknosis and necrosis (2-3 dpi) and the stomach contained blood in the lumen (6 dpi).
MAIN FINDINGS Squirrel monkeys reliably reproduced the responses observed in human cases of yellow fever and, therefore,
constitute an excellent experimental model for studies on the pathophysiology of the disease. |
| format |
Artigo |
| author |
Ferreira, Milene Silveira |
| author2 |
Bezerra Junior, Pedro Soares Cerqueira, Val?ria Duarte Rivero, Gabriela Riet Correa Oliveira Junior, Carlos Alberto Castro, Paulo Henrique Gomes Silva, Gilmara Abreu da Silva, Wellington Bandeira da Imbeloni, Aline Amaral Sousa, Jorge Rodrigues Ara?jo, Ana Paula Sousa Silva, Franko de Arruda e Tesh, Robert B Quaresma, Juarez Ant?nio Sim?es Vasconcelos, Pedro Fernando da Costa |
| author2Str |
Bezerra Junior, Pedro Soares Cerqueira, Val?ria Duarte Rivero, Gabriela Riet Correa Oliveira Junior, Carlos Alberto Castro, Paulo Henrique Gomes Silva, Gilmara Abreu da Silva, Wellington Bandeira da Imbeloni, Aline Amaral Sousa, Jorge Rodrigues Ara?jo, Ana Paula Sousa Silva, Franko de Arruda e Tesh, Robert B Quaresma, Juarez Ant?nio Sim?es Vasconcelos, Pedro Fernando da Costa |
| title |
Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy |
| title_short |
Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy |
| title_full |
Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy |
| title_fullStr |
Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy |
| title_full_unstemmed |
Experimental yellow fever virus infection in the squirrel monkey (Saimiri spp.) I: gross anatomical and histopathological findings in organs at necropsy |
| title_sort |
experimental yellow fever virus infection in the squirrel monkey (saimiri spp.) i: gross anatomical and histopathological findings in organs at necropsy |
| publisher |
Instituto Oswaldo Cruz |
| publishDate |
2020 |
| url |
http://patua.iec.gov.br//handle/iec/4202 |
| _version_ |
1717584539218345984 |
| score |
11.755432 |