First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon

Santos, Francisco J. A; Nascimento, Luciana de C?ssia Silva do; Silva, Wellington Bandeira da; Oliveira, Luciana P; Santos, Walter Souza; Aguiar, D?lia Cristina Figueira; Santos, Lourdes Maria Garcez dos

Artigo

First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon

Abstract: The American cutaneous (CL) and visceral leishmaniasis (VL) are zooanthroponoses transmitted by sand flies. Brazil records thousands of human leishmaniasis cases annually. Dogs are reservoirs of Leishmania infantum, which causes VL, but their role in the transmission cycle of CL is debatab...

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Autor principal:	Santos, Francisco J. A
Outros Autores:	Nascimento, Luciana de C?ssia Silva do, Silva, Wellington Bandeira da, Oliveira, Luciana P, Santos, Walter Souza, Aguiar, D?lia Cristina Figueira, Santos, Lourdes Maria Garcez dos
Grau:	Artigo
Idioma:	eng
Publicado em:	MDPI 2020
Assuntos:	Leishmania guyanensis / parasitologia Leishmaniose / etiologia Reservat?rios de Doen?as / veterin?ria C?es / parasitologia Tom?-A?u (PA)
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4203

id	ir-iec-4203
recordtype	dspace
spelling	ir-iec-42032021-07-20T12:12:34Z First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon Santos, Francisco J. A Nascimento, Luciana de C?ssia Silva do Silva, Wellington Bandeira da Oliveira, Luciana P Santos, Walter Souza Aguiar, D?lia Cristina Figueira Santos, Lourdes Maria Garcez dos Leishmania guyanensis / parasitologia Leishmaniose / etiologia Reservat?rios de Doen?as / veterin?ria C?es / parasitologia Tom?-A?u (PA) Abstract: The American cutaneous (CL) and visceral leishmaniasis (VL) are zooanthroponoses transmitted by sand flies. Brazil records thousands of human leishmaniasis cases annually. Dogs are reservoirs of Leishmania infantum, which causes VL, but their role in the transmission cycle of CL is debatable. Wild mammals are considered reservoirs of the aetiological agents of CL (Leishmania spp.). Objective: To describe the aetiology of leishmaniasis in dogs in an endemic area for CL and VL inthe Amazon, Brazil. Methods: Clinical evaluation and blood collection of 40 dogs from the villages Ubim (20) and Socorro (20), city of Tom?-A?u, state of Par?, were carried out. The DNA extracted from the blood was used for PCR with Leishmania-specific primers targeting the hsp70-234 gene sequence. Products were sequenced (ABI3500XL), and the sequences were aligned, edited (BioEdit), and analyzed (Blastn). Results: Of the 34 amplified samples, 21 were sequenced, namely Leishmania infantum (12), L. guyanensis (5), L. braziliensis (3), and Leishmania sp. (01). Conclusion: Given the diversity of circulating pathogens, elucidation of the role of the dog in the Leishmania spp. cycle in Amazonian villages is imperative to the surveillance of CL in the region. We present the first report in Brazil, confirmed by sequencing, of canine infection by L. guyanensis, a species highly resistant to treatment in humans, with the drug of first choice (Glucantime?) Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior?Brasil (CAPES) / Programa de P?s-gradua??o em Epidemiologia e Vigil?ncia em Sa?de (PPGEVS/IEC/SVS/MS) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq), w Instituto Evandro Chagas/SVS/MS 2020-11-23T17:46:11Z 2020-11-23T17:46:11Z 2020 Artigo SANTOS, Francisco J. A. et al. First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon. International Journal of Environmental Research and Public Health, v. 17, n. 22, p. 1-9, Nov. 2020. 1660-4601 http://patua.iec.gov.br//handle/iec/4203 10.3390/ijerph17228488 eng Acesso Aberto application/pdf MDPI
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Leishmania guyanensis / parasitologia Leishmaniose / etiologia Reservat?rios de Doen?as / veterin?ria C?es / parasitologia Tom?-A?u (PA)
spellingShingle	Leishmania guyanensis / parasitologia Leishmaniose / etiologia Reservat?rios de Doen?as / veterin?ria C?es / parasitologia Tom?-A?u (PA) Santos, Francisco J. A First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon
topic_facet	Leishmania guyanensis / parasitologia Leishmaniose / etiologia Reservat?rios de Doen?as / veterin?ria C?es / parasitologia Tom?-A?u (PA)
description	Abstract: The American cutaneous (CL) and visceral leishmaniasis (VL) are zooanthroponoses transmitted by sand flies. Brazil records thousands of human leishmaniasis cases annually. Dogs are reservoirs of Leishmania infantum, which causes VL, but their role in the transmission cycle of CL is debatable. Wild mammals are considered reservoirs of the aetiological agents of CL (Leishmania spp.). Objective: To describe the aetiology of leishmaniasis in dogs in an endemic area for CL and VL inthe Amazon, Brazil. Methods: Clinical evaluation and blood collection of 40 dogs from the villages Ubim (20) and Socorro (20), city of Tom?-A?u, state of Par?, were carried out. The DNA extracted from the blood was used for PCR with Leishmania-specific primers targeting the hsp70-234 gene sequence. Products were sequenced (ABI3500XL), and the sequences were aligned, edited (BioEdit), and analyzed (Blastn). Results: Of the 34 amplified samples, 21 were sequenced, namely Leishmania infantum (12), L. guyanensis (5), L. braziliensis (3), and Leishmania sp. (01). Conclusion: Given the diversity of circulating pathogens, elucidation of the role of the dog in the Leishmania spp. cycle in Amazonian villages is imperative to the surveillance of CL in the region. We present the first report in Brazil, confirmed by sequencing, of canine infection by L. guyanensis, a species highly resistant to treatment in humans, with the drug of first choice (Glucantime?)
format	Artigo
author	Santos, Francisco J. A
author2	Nascimento, Luciana de C?ssia Silva do Silva, Wellington Bandeira da Oliveira, Luciana P Santos, Walter Souza Aguiar, D?lia Cristina Figueira Santos, Lourdes Maria Garcez dos
author2Str	Nascimento, Luciana de C?ssia Silva do Silva, Wellington Bandeira da Oliveira, Luciana P Santos, Walter Souza Aguiar, D?lia Cristina Figueira Santos, Lourdes Maria Garcez dos
title	First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon
title_short	First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon
title_full	First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon
title_fullStr	First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon
title_full_unstemmed	First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon
title_sort	first report of canine infection by leishmania (viannia) guyanensis in the brazilian amazon
publisher	MDPI
publishDate	2020
url	http://patua.iec.gov.br//handle/iec/4203
_version_	1717584429977698304
score	11.755432

First report of canine infection by leishmania (Viannia) guyanensis in the brazilian amazon

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