Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon

Ramos, Patr?cia Karla Santos; Gon?alves, Lucas Pantoja; Alves, Ana Camila Oliveira; Casseb, Samir Mansour Moraes; Lima, Luciana Vieira do R?go; Campos, Marliane Batista; Santos, Thiago Vasconcelos dos; Silveira, Fernando Tobias

Artigo

Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon

The clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon has been defined using DTH/IFAT-IgG immune assays and the clinical statuses of infected individuals, revealing five profiles: three asymptomatic [Asymptomatic Infection (AI), Subclinical...

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Autor principal:	Ramos, Patr?cia Karla Santos
Outros Autores:	Gon?alves, Lucas Pantoja, Alves, Ana Camila Oliveira, Casseb, Samir Mansour Moraes, Lima, Luciana Vieira do R?go, Campos, Marliane Batista, Santos, Thiago Vasconcelos dos, Silveira, Fernando Tobias
Grau:	Artigo
Idioma:	eng
Publicado em:	Elsevier 2021
Assuntos:	Leishmania infantum / isolamento & purifica??o Urina / parasitologia Rea??o em Cadeia da Polimerase / M?todos qPCR
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4237

id	ir-iec-4237
recordtype	dspace
spelling	ir-iec-42372021-02-03T16:49:48Z Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon Ramos, Patr?cia Karla Santos Gon?alves, Lucas Pantoja Alves, Ana Camila Oliveira Casseb, Samir Mansour Moraes Lima, Luciana Vieira do R?go Campos, Marliane Batista Santos, Thiago Vasconcelos dos Silveira, Fernando Tobias Leishmania infantum / isolamento & purifica??o Urina / parasitologia Rea??o em Cadeia da Polimerase / M?todos qPCR The clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon has been defined using DTH/IFAT-IgG immune assays and the clinical statuses of infected individuals, revealing five profiles: three asymptomatic [Asymptomatic Infection (AI), Subclinical Resistant Infection (SRI), and Indeterminate Initial Infection (III)], and two symptomatic profiles [Subclinical Oligosymptomatic Infection (SOI) and Symptomatic Infection (SI = American visceral leishmaniasis/AVL)]. We evaluated the diagnostic potential of urine qPCR over the entire spectrum of infection. Resine Instagene Matrix? was used for DNA extraction from urinary sediment, with amplification carried out using SYBR? Green Taq with the RV1 and RV2 primers. We examined urine samples from 151 individuals from an endemic area of AVL in Par? State in the Brazilian Amazon, including: 91 (60.3%) with diagnoses of previous infections [13 (14.3%) sharing the AI profile, 13 (14.3%) with the SRI profile, 43 (47.2%) with III, 12 (13.2%) with SI (treated AVL), and 10 (11%) with SI (untreated AVL)]; sixty (39.7%) were DTH(-)/IFAT-IgG(-) (the uninfected group). The urine qPCR was positive in 61.5% of both the AI and SRI profiles, 65% of the III profile, 50% of treated AVL, 100% of untreated AVL, and 6.7% of the uninfected group. Those results confirmed the urine qPCR diagnosis in 100% of untreated AVL cases as well as in more than 60% of the cases with asymptomatic AI, SRI, and III profiles - indicating it as a promising tool for monitoring the evolution of human L. (L.) infantum chagasi-infections in endemic areas. 2021-02-03T14:10:47Z 2021-02-03T14:10:47Z 2021 Artigo RAMOS, Patr?cia Karla Santos et al. Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon. Parasitology International, v. 81, n. xx, p. xx, Apr. 2021. 1383-5769 http://patua.iec.gov.br//handle/iec/4237 10.1016/j.parint.2020.102273 eng Acesso Embargado application/pdf Elsevier
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Leishmania infantum / isolamento & purifica??o Urina / parasitologia Rea??o em Cadeia da Polimerase / M?todos qPCR
spellingShingle	Leishmania infantum / isolamento & purifica??o Urina / parasitologia Rea??o em Cadeia da Polimerase / M?todos qPCR Ramos, Patr?cia Karla Santos Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon
topic_facet	Leishmania infantum / isolamento & purifica??o Urina / parasitologia Rea??o em Cadeia da Polimerase / M?todos qPCR
description	The clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon has been defined using DTH/IFAT-IgG immune assays and the clinical statuses of infected individuals, revealing five profiles: three asymptomatic [Asymptomatic Infection (AI), Subclinical Resistant Infection (SRI), and Indeterminate Initial Infection (III)], and two symptomatic profiles [Subclinical Oligosymptomatic Infection (SOI) and Symptomatic Infection (SI = American visceral leishmaniasis/AVL)]. We evaluated the diagnostic potential of urine qPCR over the entire spectrum of infection. Resine Instagene Matrix? was used for DNA extraction from urinary sediment, with amplification carried out using SYBR? Green Taq with the RV1 and RV2 primers. We examined urine samples from 151 individuals from an endemic area of AVL in Par? State in the Brazilian Amazon, including: 91 (60.3%) with diagnoses of previous infections [13 (14.3%) sharing the AI profile, 13 (14.3%) with the SRI profile, 43 (47.2%) with III, 12 (13.2%) with SI (treated AVL), and 10 (11%) with SI (untreated AVL)]; sixty (39.7%) were DTH(-)/IFAT-IgG(-) (the uninfected group). The urine qPCR was positive in 61.5% of both the AI and SRI profiles, 65% of the III profile, 50% of treated AVL, 100% of untreated AVL, and 6.7% of the uninfected group. Those results confirmed the urine qPCR diagnosis in 100% of untreated AVL cases as well as in more than 60% of the cases with asymptomatic AI, SRI, and III profiles - indicating it as a promising tool for monitoring the evolution of human L. (L.) infantum chagasi-infections in endemic areas.
format	Artigo
author	Ramos, Patr?cia Karla Santos
author2	Gon?alves, Lucas Pantoja Alves, Ana Camila Oliveira Casseb, Samir Mansour Moraes Lima, Luciana Vieira do R?go Campos, Marliane Batista Santos, Thiago Vasconcelos dos Silveira, Fernando Tobias
author2Str	Gon?alves, Lucas Pantoja Alves, Ana Camila Oliveira Casseb, Samir Mansour Moraes Lima, Luciana Vieira do R?go Campos, Marliane Batista Santos, Thiago Vasconcelos dos Silveira, Fernando Tobias
title	Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon
title_short	Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon
title_full	Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon
title_fullStr	Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon
title_full_unstemmed	Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon
title_sort	urine qpcr diagnoses over the entire clinical-immunological spectrum of human leishmania (l.) infantum chagasi-infections in the brazilian amazon
publisher	Elsevier
publishDate	2021
url	http://patua.iec.gov.br//handle/iec/4237
_version_	1717584456244527104
score	11.687526

Urine qPCR diagnoses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi-infections in the Brazilian Amazon

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