Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats

Salazar, Marielba de los ?ngeles Rodr?guez; Urbina, Glides Rafael Olivo; Cunha, V?nia Maria Borges; Bezerra, Fernanda Wariss Figueiredo; Dias, Michelle Nerissa Coelho; Santos, Ijair Rog?rio; Teixeira, Bruno Jos? Brito; Costa, Wanessa Almeida; Leal, Walace Gomes; Souza, Jesus Nazareno Silva e; Silva, Silvia Helena Marques; Carvalho Junior, Raul Nunes

Artigo

Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats

Cissus sicyoides L. is a plant that has great anti-inflammatory activity, due to the presence of phenolic compounds. The objective of this work was to identify such substances by HPLC and to evaluate the cytotoxic effect on human red blood cells, of the C. sicyoides extract obtained by supercritical...

ver descrição completa

Autor principal:	Salazar, Marielba de los ?ngeles Rodr?guez
Outros Autores:	Urbina, Glides Rafael Olivo, Cunha, V?nia Maria Borges, Bezerra, Fernanda Wariss Figueiredo, Dias, Michelle Nerissa Coelho, Santos, Ijair Rog?rio, Teixeira, Bruno Jos? Brito, Costa, Wanessa Almeida, Leal, Walace Gomes, Souza, Jesus Nazareno Silva e, Silva, Silvia Helena Marques, Carvalho Junior, Raul Nunes
Grau:	Artigo
Idioma:	eng
Publicado em:	Elsevier 2021
Assuntos:	Plantas Medicinais / qu?mica Cissus / an?lise Cissus / qu?mica Compostos Fen?licos / an?lise Extratos Vegetais / uso terap?utico Cromatografia L?quida de Alta Press?o / m?todos
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4238

id	ir-iec-4238
recordtype	dspace
spelling	ir-iec-42382021-02-03T17:50:57Z Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats Salazar, Marielba de los ?ngeles Rodr?guez Urbina, Glides Rafael Olivo Cunha, V?nia Maria Borges Bezerra, Fernanda Wariss Figueiredo Dias, Michelle Nerissa Coelho Santos, Ijair Rog?rio Teixeira, Bruno Jos? Brito Costa, Wanessa Almeida Leal, Walace Gomes Souza, Jesus Nazareno Silva e Silva, Silvia Helena Marques Carvalho Junior, Raul Nunes Plantas Medicinais / qu?mica Cissus / an?lise Cissus / qu?mica Compostos Fen?licos / an?lise Extratos Vegetais / uso terap?utico Cromatografia L?quida de Alta Press?o / m?todos Cissus sicyoides L. is a plant that has great anti-inflammatory activity, due to the presence of phenolic compounds. The objective of this work was to identify such substances by HPLC and to evaluate the cytotoxic effect on human red blood cells, of the C. sicyoides extract obtained by supercritical extraction, using CO2 and EtOH as co-solvent, in comparison to the extract obtained by conventional extraction (Soxhlet), as well as, to evaluate its anti-inflammatory effect on spinal cord injury (SCI) in adult rats. In HPLC analysis, the UV/Visible spectral data revealed compounds with absorption bands in wavelengths characteristic of glycosylated flavonoids. The supercritical extract did not show cytotoxic effect, as there was no rupture of red blood cell membranes. The in vivo test demonstrated reduction in cell concentration in the lesion surrounding area in treated animals, suggesting an anti-inflammatory effect of the supercritical extract on the SCI model. 2021-02-03T17:10:47Z 2021-02-03T17:10:47Z 2021 Artigo SALAZAR, Marielba de los ?ngeles Rodr?guez et al. Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats. The Journal of Supercritical Fluids, v. 169, n. xx, p. xx, Feb. 2021. 0896-8446 http://patua.iec.gov.br//handle/iec/4238 10.1016/j.supflu.2020.105105 eng Acesso Embargado application/pdf Elsevier
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Plantas Medicinais / qu?mica Cissus / an?lise Cissus / qu?mica Compostos Fen?licos / an?lise Extratos Vegetais / uso terap?utico Cromatografia L?quida de Alta Press?o / m?todos
spellingShingle	Plantas Medicinais / qu?mica Cissus / an?lise Cissus / qu?mica Compostos Fen?licos / an?lise Extratos Vegetais / uso terap?utico Cromatografia L?quida de Alta Press?o / m?todos Salazar, Marielba de los ?ngeles Rodr?guez Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
topic_facet	Plantas Medicinais / qu?mica Cissus / an?lise Cissus / qu?mica Compostos Fen?licos / an?lise Extratos Vegetais / uso terap?utico Cromatografia L?quida de Alta Press?o / m?todos
description	Cissus sicyoides L. is a plant that has great anti-inflammatory activity, due to the presence of phenolic compounds. The objective of this work was to identify such substances by HPLC and to evaluate the cytotoxic effect on human red blood cells, of the C. sicyoides extract obtained by supercritical extraction, using CO2 and EtOH as co-solvent, in comparison to the extract obtained by conventional extraction (Soxhlet), as well as, to evaluate its anti-inflammatory effect on spinal cord injury (SCI) in adult rats. In HPLC analysis, the UV/Visible spectral data revealed compounds with absorption bands in wavelengths characteristic of glycosylated flavonoids. The supercritical extract did not show cytotoxic effect, as there was no rupture of red blood cell membranes. The in vivo test demonstrated reduction in cell concentration in the lesion surrounding area in treated animals, suggesting an anti-inflammatory effect of the supercritical extract on the SCI model.
format	Artigo
author	Salazar, Marielba de los ?ngeles Rodr?guez
author2	Urbina, Glides Rafael Olivo Cunha, V?nia Maria Borges Bezerra, Fernanda Wariss Figueiredo Dias, Michelle Nerissa Coelho Santos, Ijair Rog?rio Teixeira, Bruno Jos? Brito Costa, Wanessa Almeida Leal, Walace Gomes Souza, Jesus Nazareno Silva e Silva, Silvia Helena Marques Carvalho Junior, Raul Nunes
author2Str	Urbina, Glides Rafael Olivo Cunha, V?nia Maria Borges Bezerra, Fernanda Wariss Figueiredo Dias, Michelle Nerissa Coelho Santos, Ijair Rog?rio Teixeira, Bruno Jos? Brito Costa, Wanessa Almeida Leal, Walace Gomes Souza, Jesus Nazareno Silva e Silva, Silvia Helena Marques Carvalho Junior, Raul Nunes
title	Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title_short	Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title_full	Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title_fullStr	Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title_full_unstemmed	Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
title_sort	cytotoxic effect of cip?-puc? (cissus sicyoides l.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats
publisher	Elsevier
publishDate	2021
url	http://patua.iec.gov.br//handle/iec/4238
_version_	1717584355233103872
score	11.755432

Cytotoxic effect of cip?-puc? (Cissus sicyoides L.) supercritical extract on human red blood cells and as anti-inflammatory in spinal cord injury in adult rats

Registros relacionados