Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies
We previously demonstrated, using the Piry virus model, that environmental enrichment promotes higher T-cell infiltration, fewer microglial changes, and faster central nervous system (CNS) virus clearance in adult mice. However, little is known about disease progression, behavioral changes, CNS cyto...
| Autor principal: | Freitas, Priscilla dos Santos Lieuthier |
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| Outros Autores: | Lima, Ana Vict?ria de Lima, Carvalho, Karina Glazianne Barbosa, Cabral, Tatyane da Silva, farias, Alexandre Maia de, Rodrigues, Ana Paula Drummond, Diniz, Daniel Guerreiro, Dinz, Cristovam Wanderley Pican?o, Diniz J?nior, Jos? Ant?nio Pican?o |
| Grau: | Artigo |
| Idioma: | eng |
| Publicado em: |
MDPI
2021
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| Assuntos: | |
| Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4250 |
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ir-iec-4250 |
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ir-iec-42502021-02-08T18:08:49Z Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies Freitas, Priscilla dos Santos Lieuthier Lima, Ana Vict?ria de Lima Carvalho, Karina Glazianne Barbosa Cabral, Tatyane da Silva farias, Alexandre Maia de Rodrigues, Ana Paula Drummond Diniz, Daniel Guerreiro Dinz, Cristovam Wanderley Pican?o Diniz J?nior, Jos? Ant?nio Pican?o Camundongos / anatomia & histologia Sistema Nervoso Central / patologia Encefalite L?mbica V?rus Cocal Ambiente Controlado Rela??o Dose-Resposta Imunol?gica We previously demonstrated, using the Piry virus model, that environmental enrichment promotes higher T-cell infiltration, fewer microglial changes, and faster central nervous system (CNS) virus clearance in adult mice. However, little is known about disease progression, behavioral changes, CNS cytokine concentration, and neuropathology in limbic encephalitis in experimental models. Using Cocal virus, we infected C57Bl6 adult mice and studied the neuroanatomical distribution of viral antigens in correlation with the microglial morphological response, measured the CNS cytokine concentration, and assessed behavioral changes. C57Bl6 adult mice were maintained in an impoverished environment (IE) or enriched environment (EE) for four months and then subjected to the open field test. Afterwards, an equal volume of normal or virus-infected brain homogenate was nasally instilled. The brains were processed to detect viral antigens and microglial morphological changes using selective immunolabeling. We demonstrated earlier significant weight loss and higher mortality in IE mice. Additionally, behavioral analysis revealed a significant influence of the environment on locomotor and exploratory activity that was associated with less neuroinvasion and a reduced microglial response. Thus, environmental enrichment was associated with a more effective immune response in a mouse model of limbic encephalitis, allowing faster viral clearance/decreased viral dissemination, reduced disease progression, and less CNS damage. This research was funded by Instituto Evandro Chagas/Minist?rio da Sa?de, Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES), grant number 1466298. 2021-02-08T17:38:48Z 2021-02-08T17:38:48Z 2021 Artigo FREITAS, Priscilla dos Santos Lieuthier et al. Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies. Viruses, v. 13, n. 1, p. 1-21, 2021. 1999-4915 http://patua.iec.gov.br//handle/iec/4250 10.3390/v13010048 eng Acesso Aberto application/pdf MDPI |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Camundongos / anatomia & histologia Sistema Nervoso Central / patologia Encefalite L?mbica V?rus Cocal Ambiente Controlado Rela??o Dose-Resposta Imunol?gica |
| spellingShingle |
Camundongos / anatomia & histologia Sistema Nervoso Central / patologia Encefalite L?mbica V?rus Cocal Ambiente Controlado Rela??o Dose-Resposta Imunol?gica Freitas, Priscilla dos Santos Lieuthier Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
| topic_facet |
Camundongos / anatomia & histologia Sistema Nervoso Central / patologia Encefalite L?mbica V?rus Cocal Ambiente Controlado Rela??o Dose-Resposta Imunol?gica |
| description |
We previously demonstrated, using the Piry virus model, that environmental enrichment promotes higher T-cell infiltration, fewer microglial changes, and faster central nervous system (CNS) virus clearance in adult mice. However, little is known about disease progression, behavioral changes, CNS cytokine concentration, and neuropathology in limbic encephalitis in experimental models. Using Cocal virus, we infected C57Bl6 adult mice and studied the neuroanatomical distribution of viral antigens in correlation with the microglial morphological response, measured the CNS cytokine concentration, and assessed behavioral changes. C57Bl6 adult mice were maintained in an impoverished environment (IE) or enriched environment (EE) for four months and then subjected to the open field test. Afterwards, an equal volume of normal or virus-infected brain homogenate was nasally instilled. The brains were processed to detect viral antigens and microglial morphological changes using selective immunolabeling. We demonstrated earlier significant weight loss and higher mortality in IE mice. Additionally, behavioral analysis revealed a significant influence of the environment on locomotor and exploratory activity that was associated with less neuroinvasion and a reduced microglial response. Thus, environmental enrichment was associated with a more effective immune response in a mouse model of limbic encephalitis, allowing faster viral clearance/decreased viral dissemination, reduced disease progression, and less CNS damage. |
| format |
Artigo |
| author |
Freitas, Priscilla dos Santos Lieuthier |
| author2 |
Lima, Ana Vict?ria de Lima Carvalho, Karina Glazianne Barbosa Cabral, Tatyane da Silva farias, Alexandre Maia de Rodrigues, Ana Paula Drummond Diniz, Daniel Guerreiro Dinz, Cristovam Wanderley Pican?o Diniz J?nior, Jos? Ant?nio Pican?o |
| author2Str |
Lima, Ana Vict?ria de Lima Carvalho, Karina Glazianne Barbosa Cabral, Tatyane da Silva farias, Alexandre Maia de Rodrigues, Ana Paula Drummond Diniz, Daniel Guerreiro Dinz, Cristovam Wanderley Pican?o Diniz J?nior, Jos? Ant?nio Pican?o |
| title |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
| title_short |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
| title_full |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
| title_fullStr |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
| title_full_unstemmed |
Limbic encephalitis brain damage induced by Cocal Virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
| title_sort |
limbic encephalitis brain damage induced by cocal virus in adult mice is reduced by environmental enrichment: neuropathological and behavioral studies |
| publisher |
MDPI |
| publishDate |
2021 |
| url |
http://patua.iec.gov.br//handle/iec/4250 |
| _version_ |
1717584456585314304 |
| score |
11.755432 |