/img alt="Imagem da capa" class="recordcover" src="""/>
Artigo
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection
Background: Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response o...
Autor principal: | Sandoval, Carmen |
---|---|
Outros Autores: | Araujo, Gabriela, Sosa, Wilfredo, Avalos, Sara, Silveira, Fernando, Corbett, Carlos, Z?niga, Concepci?n, Laurenti, Marcia |
Grau: | Artigo |
Idioma: | eng |
Publicado em: |
BMC
2021
|
Assuntos: | |
Acesso em linha: |
http://patua.iec.gov.br//handle/iec/4272 |
id |
ir-iec-4272 |
---|---|
recordtype |
dspace |
spelling |
ir-iec-42722021-03-31T17:25:56Z In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection Sandoval, Carmen Araujo, Gabriela Sosa, Wilfredo Avalos, Sara Silveira, Fernando Corbett, Carlos Z?niga, Concepci?n Laurenti, Marcia Leishmaniose Cut?nea / patologia Leishmania infantum / parasitologia Desenluvamentos Cut?neos Imuno-Histoqu?mica Macr?fagos Honduras Background: Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL. Methods: Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma. Results: Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-?, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8+ T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS+, a response that could be mediated by IFN-?. Conclusion: Our study improves knowledge of the cellular immune response in non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8+ T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL. The present research was supported by the S?o Paulo Research Foundation (FAPESP) grants #2014/50315-0, #2015/01154-7, #2017/24834-9, #2018/04698-6, Direcci?n de Investigacion y Posgrados de la UNAH grant #02?2015. GA is the recipiente of a scholarship from CAPES (Social Demand) and Laboratorio de Patologia de Molestias Infecciosas (LIM50 HC-FMUSP). ML is the recipient of a research fellowship from the National Council for Scientific and Technological Development (CNPq), grant # 302174/2017-6. 2021-03-29T19:07:08Z 2021-03-29T19:07:08Z 2021 Artigo SANDOVAL, Carmen et al. In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 27, e. 20200149, Feb. 2021. 1678-9199 http://patua.iec.gov.br//handle/iec/4272 10.1590/1678-9199-jvatitd-2020-0149 eng Acesso Aberto application/pdf BMC |
institution |
Instituto Evandro Chagas (IEC) |
collection |
PATUA |
language |
eng |
topic |
Leishmaniose Cut?nea / patologia Leishmania infantum / parasitologia Desenluvamentos Cut?neos Imuno-Histoqu?mica Macr?fagos Honduras |
spellingShingle |
Leishmaniose Cut?nea / patologia Leishmania infantum / parasitologia Desenluvamentos Cut?neos Imuno-Histoqu?mica Macr?fagos Honduras Sandoval, Carmen In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
topic_facet |
Leishmaniose Cut?nea / patologia Leishmania infantum / parasitologia Desenluvamentos Cut?neos Imuno-Histoqu?mica Macr?fagos Honduras |
description |
Background: Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL.
Methods: Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma.
Results: Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-?, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8+ T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS+, a response that could be mediated by IFN-?.
Conclusion: Our study improves knowledge of the cellular immune response in non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8+ T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL. |
format |
Artigo |
author |
Sandoval, Carmen |
author2 |
Araujo, Gabriela Sosa, Wilfredo Avalos, Sara Silveira, Fernando Corbett, Carlos Z?niga, Concepci?n Laurenti, Marcia |
author2Str |
Araujo, Gabriela Sosa, Wilfredo Avalos, Sara Silveira, Fernando Corbett, Carlos Z?niga, Concepci?n Laurenti, Marcia |
title |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title_short |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title_full |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title_fullStr |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title_full_unstemmed |
In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection |
title_sort |
in situ cellular immune response in non-ulcerated skin lesions due to leishmania (l.) infantum chagasi infection |
publisher |
BMC |
publishDate |
2021 |
url |
http://patua.iec.gov.br//handle/iec/4272 |
_version_ |
1717584540336128000 |
score |
11.687526 |