Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil
Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequen...
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ir-iec-4299 |
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ir-iec-42992021-05-03T15:14:12Z Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil Adelino, Talita ?mile Ribeiro Giovanetti, Marta Fonseca, Vagner Xavier, Joilson Abreu, ?lvaro Salgado de Nascimento, Valdinete Alves do Demarchi, Luiz Henrique Ferraz Oliveira, Marluce Aparecida Assun??o Silva, Vin?cius Lemos da Mello, Arabela Leal e Silva de Cunha, Gabriel Muricy Santos, Roselene Hans Oliveira, Elaine Cristina de Chamon J?nior, Jorge Ant?nio Iani, Felipe Campos de Melo Filippis, Ana Maria Bispo de Abreu, Andr? Luiz de Jesus, Ronaldo de Albuquerque, Carlos Frederico Campelo de Rico, Jairo Mendez Said, Rodrigo Fabiano do Carmo Silva, Josc?lio Aguiar Moura, Noely Fabiana Oliveira de Leite, Priscila Frutuoso, L?via Carla Vinhal Haddad, Simone Kashima Mart?nez, Alexander Barreto, Fernanda Khouri Vazquez, Cynthia Carolina Cunha, Rivaldo Ven?ncio da Ara?jo, Emerson Luiz Lima Tosta, Stephane Fraga de Oliveira Fabri, Allison de Ara?jo Chalhoub, Fl?via Lowen Levy Lemos, Poliana da Silva Bruycker-Nogueira, Fernanda de Lichs, Gislene Garcia de Castro Zardin, Marina Castilho Souza Umaki Segovia, F?tima Mar?a Cardozo Gon?alves, Crhistinne Cavalheiro Maymone Grillo, Zoraida Del Carmen Fernandez Slavov, Svetoslav Nanev Pereira, Luiz Augusto Mendon?a, Ana Fl?via Pereira, Felicidade Mota Magalh?es, Jurandy J?nior Ferraz de Santos J?nior, Agenor de Castro Moreira dos Lima, Maric?lia Maia de Nogueira, Rita Maria Ribeiro G?es-Neto, Arist?teles Azevedo, Vasco Ariston de Carvalho Ramalho, Dario Brock Oliveira, Wanderson Kleber Macario, Eduardo Marques Medeiros, Arnaldo Correia de Pimentel, Victor Holmes, Edward C Oliveira, Tulio de Louren?o, Jos? Alcantara, Luiz Carlos Junior Cerqueira, Erenilde Marques de Graf, Tiago Ramalho, Walter Navegantes, Wildo Reis, Renato Barbosa Duarte, Clara Guerra Pereira, Maria Alves Silva, Paulo Eduardo de Souza da Souza, Raoni Almeida de Pauvolid-Corr?a, Alex Paiva, Anne Aline Pereira de Fritsch, Hegger Machado Mares-Guia, Maria Ang?lica Torres, Maria Celeste Lima, Maur?cio Teixeira Sequeira, Patr?cia Marques, William de Almeida Jesus, Jorlan Fernandes de Naveca, Felipe Gomes Silva, Alessandra Lima Pinto, Anne Cybelle Jaiswal, Arun Kumar Lopes, ?lisson Nogueira Costa, Francielly Morais Rodrigues da Quintanilha-Peixoto, Gabriel Soares, Gilson Carlos Fonseca, Paula Luize Camargos Souza, Renan Pedra de Kato, Rodrigo Bentes Santos, Rodrigo Profeta Silveira Tiwari, Sandeep Nogueira, Wylerson Guimar?es Santos, Beatriz Senra ?lvares da Silva Bueno, Bruna Lopes Siqueira, Isadora Cristina de Valle, Lourdes Farre Borba, Melina Mosquera Navarro Mazzetto, Alix Sandra Aguiar, Francisco de Assis Ara?jo Gomes, Irenio da Silva Abrantes, Jayra Juliana Paiva Alves Watanabe, Luiz Takao Rego, Marta Ferreira da Silva Nardy, Vanessa Brand?o Aguiar, Shirlei Ferreira de Santos, Fabiana Cristina Pereira dos Queiroz, Alice Louize Nunes Nunes, Bruno Tardelli Diniz Martins, L?via Car?cio Nunes, Marcio Roberto Teixeira Salles, Fl?via Cristina da Silva Claro, Ingra Morales Jesus, Jaqueline Goes de C?ndido, Darlan da Silva Fabbri, Cintia Marcela Gonz?lez, Claudia Sa?z, Lisseth Chen-Germ?n, Mar?a Barrera, Jaime Lagos Ram?rez-Gonz?lez, Jos? Ernesto Campos, Josefina Faller, Noelia Morel Villalobos, Marta Eugenia V?quez Kaslin, Roberto Cisneros, Silvia Paola Salgado Aburjaile, Fl?via Figueira Amaral, Carolina Dourado Freire, Danielle Bandeira Costa de Sousa Cruz, Laura Nogueira Mattos, Daniel Landeira, Leandro Ferreira Lopes Menezes, Mariane Talon de Orioli, Ieda Maria Ferraz, Ariane Coelho Oliveira, Daiane Teixeira de Reis, Alexandre Barbosa Cota, Renata Guerra de S? Bezerra, Rafael dos Santos Falc?o, Melissa Barreto Carvalho, Rodrigo Dias de Oliveira Dengue / virologia Dengue / transmiss?o Varia??o Estrutural do Genoma Sequenciamento por Nanoporos V?rus da Dengue / patogenicidade Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015?2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses. This work was supported by Decit, SCTIE, Brazilian Ministry of Health, Conselho Nacional de Desenvolvimento Cient?fico - CNPq (440685/ 2016-8, 440856/2016-7 and 421598/2018-2), Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES - (88887.130716/2016-00), European Union?s Horizon 2020 Research and Innovation Programme under ZIKAlliance Grant Agreement (734548), STARBIOS (709517), Funda??o de Amparo ? Pesquisa do Estado do Rio de Janeiro ? FAPERJ (E-26/2002.930/2016), International Development Research Centre (IDRC) Canada (108411-001), European Union?s Horizon 2020 under grant agreements ZIKACTION (734857) and ZIKAPLAN (734548). 2021-05-03T13:09:32Z 2021-05-03T13:09:32Z 2021 Artigo ADELINO, Talita ?mile Ribeiro et al. Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil. Nature Communications, v. 12, n. 1. p. 1-12, Dec. 2021. 2041-1723 http://patua.iec.gov.br//handle/iec/4299 10.1038/s41467-021-22607-0 eng Acesso Aberto application/pdf Nature Research |
| institution |
Instituto Evandro Chagas (IEC) |
| collection |
PATUA |
| language |
eng |
| topic |
Dengue / virologia Dengue / transmiss?o Varia??o Estrutural do Genoma Sequenciamento por Nanoporos V?rus da Dengue / patogenicidade |
| spellingShingle |
Dengue / virologia Dengue / transmiss?o Varia??o Estrutural do Genoma Sequenciamento por Nanoporos V?rus da Dengue / patogenicidade Adelino, Talita ?mile Ribeiro Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| topic_facet |
Dengue / virologia Dengue / transmiss?o Varia??o Estrutural do Genoma Sequenciamento por Nanoporos V?rus da Dengue / patogenicidade |
| description |
Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015?2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses. |
| format |
Artigo |
| author |
Adelino, Talita ?mile Ribeiro |
| author2 |
Giovanetti, Marta Fonseca, Vagner Xavier, Joilson Abreu, ?lvaro Salgado de Nascimento, Valdinete Alves do Demarchi, Luiz Henrique Ferraz Oliveira, Marluce Aparecida Assun??o Silva, Vin?cius Lemos da Mello, Arabela Leal e Silva de Cunha, Gabriel Muricy Santos, Roselene Hans Oliveira, Elaine Cristina de Chamon J?nior, Jorge Ant?nio Iani, Felipe Campos de Melo Filippis, Ana Maria Bispo de Abreu, Andr? Luiz de Jesus, Ronaldo de Albuquerque, Carlos Frederico Campelo de Rico, Jairo Mendez Said, Rodrigo Fabiano do Carmo Silva, Josc?lio Aguiar Moura, Noely Fabiana Oliveira de Leite, Priscila Frutuoso, L?via Carla Vinhal Haddad, Simone Kashima Mart?nez, Alexander Barreto, Fernanda Khouri Vazquez, Cynthia Carolina Cunha, Rivaldo Ven?ncio da Ara?jo, Emerson Luiz Lima Tosta, Stephane Fraga de Oliveira Fabri, Allison de Ara?jo Chalhoub, Fl?via Lowen Levy Lemos, Poliana da Silva Bruycker-Nogueira, Fernanda de Lichs, Gislene Garcia de Castro Zardin, Marina Castilho Souza Umaki Segovia, F?tima Mar?a Cardozo Gon?alves, Crhistinne Cavalheiro Maymone Grillo, Zoraida Del Carmen Fernandez Slavov, Svetoslav Nanev Pereira, Luiz Augusto Mendon?a, Ana Fl?via Pereira, Felicidade Mota Magalh?es, Jurandy J?nior Ferraz de Santos J?nior, Agenor de Castro Moreira dos Lima, Maric?lia Maia de Nogueira, Rita Maria Ribeiro G?es-Neto, Arist?teles Azevedo, Vasco Ariston de Carvalho Ramalho, Dario Brock Oliveira, Wanderson Kleber Macario, Eduardo Marques Medeiros, Arnaldo Correia de Pimentel, Victor Holmes, Edward C Oliveira, Tulio de Louren?o, Jos? Alcantara, Luiz Carlos Junior Cerqueira, Erenilde Marques de Graf, Tiago Ramalho, Walter Navegantes, Wildo Reis, Renato Barbosa Duarte, Clara Guerra Pereira, Maria Alves Silva, Paulo Eduardo de Souza da Souza, Raoni Almeida de Pauvolid-Corr?a, Alex Paiva, Anne Aline Pereira de Fritsch, Hegger Machado Mares-Guia, Maria Ang?lica Torres, Maria Celeste Lima, Maur?cio Teixeira Sequeira, Patr?cia Marques, William de Almeida Jesus, Jorlan Fernandes de Naveca, Felipe Gomes Silva, Alessandra Lima Pinto, Anne Cybelle Jaiswal, Arun Kumar Lopes, ?lisson Nogueira Costa, Francielly Morais Rodrigues da Quintanilha-Peixoto, Gabriel Soares, Gilson Carlos Fonseca, Paula Luize Camargos Souza, Renan Pedra de Kato, Rodrigo Bentes Santos, Rodrigo Profeta Silveira Tiwari, Sandeep Nogueira, Wylerson Guimar?es Santos, Beatriz Senra ?lvares da Silva Bueno, Bruna Lopes Siqueira, Isadora Cristina de Valle, Lourdes Farre Borba, Melina Mosquera Navarro Mazzetto, Alix Sandra Aguiar, Francisco de Assis Ara?jo Gomes, Irenio da Silva Abrantes, Jayra Juliana Paiva Alves Watanabe, Luiz Takao Rego, Marta Ferreira da Silva Nardy, Vanessa Brand?o Aguiar, Shirlei Ferreira de Santos, Fabiana Cristina Pereira dos Queiroz, Alice Louize Nunes Nunes, Bruno Tardelli Diniz Martins, L?via Car?cio Nunes, Marcio Roberto Teixeira Salles, Fl?via Cristina da Silva Claro, Ingra Morales Jesus, Jaqueline Goes de C?ndido, Darlan da Silva Fabbri, Cintia Marcela Gonz?lez, Claudia Sa?z, Lisseth Chen-Germ?n, Mar?a Barrera, Jaime Lagos Ram?rez-Gonz?lez, Jos? Ernesto Campos, Josefina Faller, Noelia Morel Villalobos, Marta Eugenia V?quez Kaslin, Roberto Cisneros, Silvia Paola Salgado Aburjaile, Fl?via Figueira Amaral, Carolina Dourado Freire, Danielle Bandeira Costa de Sousa Cruz, Laura Nogueira Mattos, Daniel Landeira, Leandro Ferreira Lopes Menezes, Mariane Talon de Orioli, Ieda Maria Ferraz, Ariane Coelho Oliveira, Daiane Teixeira de Reis, Alexandre Barbosa Cota, Renata Guerra de S? Bezerra, Rafael dos Santos Falc?o, Melissa Barreto Carvalho, Rodrigo Dias de Oliveira |
| author2Str |
Giovanetti, Marta Fonseca, Vagner Xavier, Joilson Abreu, ?lvaro Salgado de Nascimento, Valdinete Alves do Demarchi, Luiz Henrique Ferraz Oliveira, Marluce Aparecida Assun??o Silva, Vin?cius Lemos da Mello, Arabela Leal e Silva de Cunha, Gabriel Muricy Santos, Roselene Hans Oliveira, Elaine Cristina de Chamon J?nior, Jorge Ant?nio Iani, Felipe Campos de Melo Filippis, Ana Maria Bispo de Abreu, Andr? Luiz de Jesus, Ronaldo de Albuquerque, Carlos Frederico Campelo de Rico, Jairo Mendez Said, Rodrigo Fabiano do Carmo Silva, Josc?lio Aguiar Moura, Noely Fabiana Oliveira de Leite, Priscila Frutuoso, L?via Carla Vinhal Haddad, Simone Kashima Mart?nez, Alexander Barreto, Fernanda Khouri Vazquez, Cynthia Carolina Cunha, Rivaldo Ven?ncio da Ara?jo, Emerson Luiz Lima Tosta, Stephane Fraga de Oliveira Fabri, Allison de Ara?jo Chalhoub, Fl?via Lowen Levy Lemos, Poliana da Silva Bruycker-Nogueira, Fernanda de Lichs, Gislene Garcia de Castro Zardin, Marina Castilho Souza Umaki Segovia, F?tima Mar?a Cardozo Gon?alves, Crhistinne Cavalheiro Maymone Grillo, Zoraida Del Carmen Fernandez Slavov, Svetoslav Nanev Pereira, Luiz Augusto Mendon?a, Ana Fl?via Pereira, Felicidade Mota Magalh?es, Jurandy J?nior Ferraz de Santos J?nior, Agenor de Castro Moreira dos Lima, Maric?lia Maia de Nogueira, Rita Maria Ribeiro G?es-Neto, Arist?teles Azevedo, Vasco Ariston de Carvalho Ramalho, Dario Brock Oliveira, Wanderson Kleber Macario, Eduardo Marques Medeiros, Arnaldo Correia de Pimentel, Victor Holmes, Edward C Oliveira, Tulio de Louren?o, Jos? Alcantara, Luiz Carlos Junior Cerqueira, Erenilde Marques de Graf, Tiago Ramalho, Walter Navegantes, Wildo Reis, Renato Barbosa Duarte, Clara Guerra Pereira, Maria Alves Silva, Paulo Eduardo de Souza da Souza, Raoni Almeida de Pauvolid-Corr?a, Alex Paiva, Anne Aline Pereira de Fritsch, Hegger Machado Mares-Guia, Maria Ang?lica Torres, Maria Celeste Lima, Maur?cio Teixeira Sequeira, Patr?cia Marques, William de Almeida Jesus, Jorlan Fernandes de Naveca, Felipe Gomes Silva, Alessandra Lima Pinto, Anne Cybelle Jaiswal, Arun Kumar Lopes, ?lisson Nogueira Costa, Francielly Morais Rodrigues da Quintanilha-Peixoto, Gabriel Soares, Gilson Carlos Fonseca, Paula Luize Camargos Souza, Renan Pedra de Kato, Rodrigo Bentes Santos, Rodrigo Profeta Silveira Tiwari, Sandeep Nogueira, Wylerson Guimar?es Santos, Beatriz Senra ?lvares da Silva Bueno, Bruna Lopes Siqueira, Isadora Cristina de Valle, Lourdes Farre Borba, Melina Mosquera Navarro Mazzetto, Alix Sandra Aguiar, Francisco de Assis Ara?jo Gomes, Irenio da Silva Abrantes, Jayra Juliana Paiva Alves Watanabe, Luiz Takao Rego, Marta Ferreira da Silva Nardy, Vanessa Brand?o Aguiar, Shirlei Ferreira de Santos, Fabiana Cristina Pereira dos Queiroz, Alice Louize Nunes Nunes, Bruno Tardelli Diniz Martins, L?via Car?cio Nunes, Marcio Roberto Teixeira Salles, Fl?via Cristina da Silva Claro, Ingra Morales Jesus, Jaqueline Goes de C?ndido, Darlan da Silva Fabbri, Cintia Marcela Gonz?lez, Claudia Sa?z, Lisseth Chen-Germ?n, Mar?a Barrera, Jaime Lagos Ram?rez-Gonz?lez, Jos? Ernesto Campos, Josefina Faller, Noelia Morel Villalobos, Marta Eugenia V?quez Kaslin, Roberto Cisneros, Silvia Paola Salgado Aburjaile, Fl?via Figueira Amaral, Carolina Dourado Freire, Danielle Bandeira Costa de Sousa Cruz, Laura Nogueira Mattos, Daniel Landeira, Leandro Ferreira Lopes Menezes, Mariane Talon de Orioli, Ieda Maria Ferraz, Ariane Coelho Oliveira, Daiane Teixeira de Reis, Alexandre Barbosa Cota, Renata Guerra de S? Bezerra, Rafael dos Santos Falc?o, Melissa Barreto Carvalho, Rodrigo Dias de Oliveira |
| title |
Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_short |
Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_full |
Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_fullStr |
Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_full_unstemmed |
Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil |
| title_sort |
field and classroom initiatives for portable sequence-based monitoring of dengue virus in brazil |
| publisher |
Nature Research |
| publishDate |
2021 |
| url |
http://patua.iec.gov.br//handle/iec/4299 |
| _version_ |
1717584406234791936 |
| score |
11.755432 |