Artigo

Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure

Background Fluoride (F) is a naturally exists in nature but several studies have indicated it as an environmental toxicant to all leaving beings. Human F exposure has increased over the years since this ion has been used by industry on foods, beverages, toothpastes and on water supply. Although F is...

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Autor principal: Puty, Bruna
Outros Autores: Bittencourt, Leonardo Oliveira, Nogueira, Iago Cesar, Buzalaf, Mar?lia Afonso Rabelo, Oliveira, Edivaldo Herculano Corr?a de, Lima, Rafael Rodrigues
Grau: Artigo
Idioma: eng
Publicado em: Public Library of Science 2021
Assuntos:
Fl?or / an?lise
Fl?or / toxicidade
Sistema Nervoso Central / citologia
Neuroglia / efeitos dos f?rmacos
Acesso em linha: http://patua.iec.gov.br//handle/iec/4334
id ir-iec-4334
recordtype dspace
spelling ir-iec-43342021-06-28T14:12:21Z Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure Puty, Bruna Bittencourt, Leonardo Oliveira Nogueira, Iago Cesar Buzalaf, Mar?lia Afonso Rabelo Oliveira, Edivaldo Herculano Corr?a de Lima, Rafael Rodrigues Fl?or / an?lise Fl?or / toxicidade Sistema Nervoso Central / citologia Neuroglia / efeitos dos f?rmacos Background Fluoride (F) is a naturally exists in nature but several studies have indicated it as an environmental toxicant to all leaving beings. Human F exposure has increased over the years since this ion has been used by industry on foods, beverages, toothpastes and on water supply. Although F is safe at optimal concentrations in water supply, human exposure to high levels could trigger neurofunctional deficits. Materials and methods In this study, human glial-like (U87) and neuronal-like (IMR-32) cells lineages were used to access F toxicity and CNS cell sensibility on both cell facing the same protocol. Cells were exposed to F over 3, 5 and 10 days on two different F concentrations. Fluoride exposed cells were evaluated by standard toxicity assays to cell viability, apoptosis, necrosis and general cell metabolism. Oxidative stress parameters were evaluated by ATP and ROS levels, lipid peroxidation, GSH/GSSG ratio and comet assay. Results No changes were observed in IMR-32 at any given time while after 10 days of exposure to 0.22?g/mL, U87 glial-like cells showed signs of toxicity such as decreased cell viability by necrosis while general cell metabolism was increased. Oxidative stress parameters were next evaluated only on U87 glial-like cells after 10 days of exposure. F induced a decrease on ATP levels while no changes were observed on reactive oxygen species and lipid peroxidation. GSH/GSSG ratio was decreased followed by DNA damage both on 0.22?g/mL F. Conclusions Our results suggest an important differential behavior of the distinct types of cells exposed to the different fluoride concentrations, pointing that the U87 glial-like cells as more susceptible to damage triggered by this ion. ? 2021 Puty et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. This study was supported by the Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico ? Brasil (CNPq) (grant no. 435093/ 2018-5) and the Coordination of Personal Improvement of Higher Education ? (CAPES) Brasil (Finance Code 001). 2021-06-28T13:38:45Z 2021-06-28T13:38:45Z 2021 Artigo PUTY, Bruna et al. Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure. PLoS ONE, v. 16, n. 6, p. 1-16, June 2021. 1932-6203 http://patua.iec.gov.br//handle/iec/4334 10.1371/journal.pone.0251200 eng Acesso Aberto application/pdf Public Library of Science
institution Instituto Evandro Chagas (IEC)
collection PATUA
language eng
topic Fl?or / an?lise
Fl?or / toxicidade
Sistema Nervoso Central / citologia
Neuroglia / efeitos dos f?rmacos
spellingShingle Fl?or / an?lise
Fl?or / toxicidade
Sistema Nervoso Central / citologia
Neuroglia / efeitos dos f?rmacos
Puty, Bruna
Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure
topic_facet Fl?or / an?lise
Fl?or / toxicidade
Sistema Nervoso Central / citologia
Neuroglia / efeitos dos f?rmacos
description Background Fluoride (F) is a naturally exists in nature but several studies have indicated it as an environmental toxicant to all leaving beings. Human F exposure has increased over the years since this ion has been used by industry on foods, beverages, toothpastes and on water supply. Although F is safe at optimal concentrations in water supply, human exposure to high levels could trigger neurofunctional deficits. Materials and methods In this study, human glial-like (U87) and neuronal-like (IMR-32) cells lineages were used to access F toxicity and CNS cell sensibility on both cell facing the same protocol. Cells were exposed to F over 3, 5 and 10 days on two different F concentrations. Fluoride exposed cells were evaluated by standard toxicity assays to cell viability, apoptosis, necrosis and general cell metabolism. Oxidative stress parameters were evaluated by ATP and ROS levels, lipid peroxidation, GSH/GSSG ratio and comet assay. Results No changes were observed in IMR-32 at any given time while after 10 days of exposure to 0.22?g/mL, U87 glial-like cells showed signs of toxicity such as decreased cell viability by necrosis while general cell metabolism was increased. Oxidative stress parameters were next evaluated only on U87 glial-like cells after 10 days of exposure. F induced a decrease on ATP levels while no changes were observed on reactive oxygen species and lipid peroxidation. GSH/GSSG ratio was decreased followed by DNA damage both on 0.22?g/mL F. Conclusions Our results suggest an important differential behavior of the distinct types of cells exposed to the different fluoride concentrations, pointing that the U87 glial-like cells as more susceptible to damage triggered by this ion. ? 2021 Puty et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
format Artigo
author Puty, Bruna
author2 Bittencourt, Leonardo Oliveira
Nogueira, Iago Cesar
Buzalaf, Mar?lia Afonso Rabelo
Oliveira, Edivaldo Herculano Corr?a de
Lima, Rafael Rodrigues
author2Str Bittencourt, Leonardo Oliveira
Nogueira, Iago Cesar
Buzalaf, Mar?lia Afonso Rabelo
Oliveira, Edivaldo Herculano Corr?a de
Lima, Rafael Rodrigues
title Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure
title_short Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure
title_full Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure
title_fullStr Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure
title_full_unstemmed Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure
title_sort human cultured imr-32 neuronal-like and u87 glial-like cells have different patterns of toxicity under fluoride exposure
publisher Public Library of Science
publishDate 2021
url http://patua.iec.gov.br//handle/iec/4334
_version_ 1717584356465180672
score 11.675608

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