Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci

Conway, David J; Machado, Ricardo Luiz Dantas; Singh, Balbir; Dessert, Patricia; Mikes, Zsuzsanna S; P?voa, Marinete Marins; Oduola, Ayoade M. J; Roper, Cally

Artigo

Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci

Comparing patterns of genetic variation at multiple loci in the genome of a species can potentially identify loci which are under selection. The large number of polymorphic microsatellites in the malaria parasite Plasmodium falciparum are available markers to screen for selectively important loci....

ver descrição completa

Autor principal:	Conway, David J
Outros Autores:	Machado, Ricardo Luiz Dantas, Singh, Balbir, Dessert, Patricia, Mikes, Zsuzsanna S, P?voa, Marinete Marins, Oduola, Ayoade M. J, Roper, Cally
Grau:	Artigo
Idioma:	eng
Publicado em:	Elsevier 2021
Assuntos:	Mal?ria Plasmodium falciparum / parasitologia Genoma / gen?tica Marcadores Gen?ticos Repeti??es de Microssat?lites
Acesso em linha:	http://patua.iec.gov.br//handle/iec/4362

id	ir-iec-4362
recordtype	dspace
spelling	ir-iec-43622021-07-19T22:58:14Z Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci Conway, David J Machado, Ricardo Luiz Dantas Singh, Balbir Dessert, Patricia Mikes, Zsuzsanna S P?voa, Marinete Marins Oduola, Ayoade M. J Roper, Cally Mal?ria Plasmodium falciparum / parasitologia Genoma / gen?tica Marcadores Gen?ticos Repeti??es de Microssat?lites Comparing patterns of genetic variation at multiple loci in the genome of a species can potentially identify loci which are under selection. The large number of polymorphic microsatellites in the malaria parasite Plasmodium falciparum are available markers to screen for selectively important loci. The Pfs48/45 gene on Chromosome 13 encodes an antigenic protein located on the surface of parasite gametes, which is a candidate for a transmission blocking vaccine. Here, genotypic data from 255 P. falciparum isolates are presented, which show that alleles and haplotypes of five single nucleotide polymorphisms (SNPs) in the Pfs48/45 gene are exceptionally skewed in frequency among different P. falciparum populations, compared with alleles at 11 microsatellite loci sampled widely from the parasite genome. Fixation indices measuring inter-population variance in allele frequencies (FST) were in the order of four to seven times higher for Pfs48/45 than for the microsatellites, whether considered (i) among populations within Africa, or (ii) among different continents. Differing mutational processes at microsatellite and SNP loci could generally affect the population structure at these different types of loci, to an unknown extent which deserves further investigation. The highly contrasting population structure may also suggest divergent selection on the amino acid sequence of Pfs48/45 in different populations, which plausibly indicates a role for the protein in determining gamete recognition and compatibility. ? 2001 Elsevier Science B.V. Wellcome Trust (Grant Ref. 055487/Z/98/Z) and the Medical Research Council (Grant Ref. G9803180). 2021-07-19T22:28:43Z 2021-07-19T22:28:43Z 2001 Artigo CONWAY, David J. et al. Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci. Molecular and Biochemical Parasitology, v. 115, n. 2, p. 145-156, July 2001. 0166-6851 http://patua.iec.gov.br//handle/iec/4362 10.1016/s0166-6851(01)00278-x. eng Acesso Aberto application/pdf Elsevier
institution	Instituto Evandro Chagas (IEC)
collection	PATUA
language	eng
topic	Mal?ria Plasmodium falciparum / parasitologia Genoma / gen?tica Marcadores Gen?ticos Repeti??es de Microssat?lites
spellingShingle	Mal?ria Plasmodium falciparum / parasitologia Genoma / gen?tica Marcadores Gen?ticos Repeti??es de Microssat?lites Conway, David J Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci
topic_facet	Mal?ria Plasmodium falciparum / parasitologia Genoma / gen?tica Marcadores Gen?ticos Repeti??es de Microssat?lites
description	Comparing patterns of genetic variation at multiple loci in the genome of a species can potentially identify loci which are under selection. The large number of polymorphic microsatellites in the malaria parasite Plasmodium falciparum are available markers to screen for selectively important loci. The Pfs48/45 gene on Chromosome 13 encodes an antigenic protein located on the surface of parasite gametes, which is a candidate for a transmission blocking vaccine. Here, genotypic data from 255 P. falciparum isolates are presented, which show that alleles and haplotypes of five single nucleotide polymorphisms (SNPs) in the Pfs48/45 gene are exceptionally skewed in frequency among different P. falciparum populations, compared with alleles at 11 microsatellite loci sampled widely from the parasite genome. Fixation indices measuring inter-population variance in allele frequencies (FST) were in the order of four to seven times higher for Pfs48/45 than for the microsatellites, whether considered (i) among populations within Africa, or (ii) among different continents. Differing mutational processes at microsatellite and SNP loci could generally affect the population structure at these different types of loci, to an unknown extent which deserves further investigation. The highly contrasting population structure may also suggest divergent selection on the amino acid sequence of Pfs48/45 in different populations, which plausibly indicates a role for the protein in determining gamete recognition and compatibility. ? 2001 Elsevier Science B.V.
format	Artigo
author	Conway, David J
author2	Machado, Ricardo Luiz Dantas Singh, Balbir Dessert, Patricia Mikes, Zsuzsanna S P?voa, Marinete Marins Oduola, Ayoade M. J Roper, Cally
author2Str	Machado, Ricardo Luiz Dantas Singh, Balbir Dessert, Patricia Mikes, Zsuzsanna S P?voa, Marinete Marins Oduola, Ayoade M. J Roper, Cally
title	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci
title_short	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci
title_full	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci
title_fullStr	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci
title_full_unstemmed	Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci
title_sort	extreme geographical fixation of variation in the plasmodium falciparum gamete surface protein gene pfs48/45 compared with microsatellite loci
publisher	Elsevier
publishDate	2021
url	http://patua.iec.gov.br//handle/iec/4362
_version_	1717584407025418240
score	11.755432

Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci

Registros relacionados