Piper aduncum L. (Pimenta de Macaco) and Piper hispidinervium C. DC. (Pimenta Longa), are aromatic plants native to the Amazon region belonging to the Piperaceae family and are characterized by producing essential oils. P. aduncum produces essential oil rich in Dilapiol, this oil has been tested as insecticide, molluscicide, fungicide, acaricide and bactericide all with positive results. P. hispidinervium, has an essential oil rich in Safrol, which is used in the chemical industry as a fixative for fragrances and as a synergistic agent for the manufacture of insecticides. The present work aimed to define and adapt such a methodology that makes artificial cross-pollination between the species possible. To perform the experiment, 2 inflorescences per plant were chosen, using 2 plants per species, totaling 8 pollinations. The spikes were isolated with wrappings and artificial cross-pollination was performed in a 1 cm-area located in the apical portion of the spike, between days 2r and 300 after the beginning of the experiment. The ears were removed from the plants when the fruits showed signs of ripeness and were ready to release the seeds. The seeds were put to germinate in vegetal soil at room temperature. They are currently being transplanted to the field to follow their development and obtain the respective analyses of the essential oils. The present work indicates that the germination index, phenological development and survival of seedlings are the same for both species, however, from the analysis of the oils it will be possible to conclude the variation of Safrole and Dilapiol between plants from forced pollination.
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