The Amazon is the largest tropical rainforest in the world and stands out for having the largest
diversity of plants, and numerous species of microorganisms, including fungus and bacteria with
huge potential for the development of new products. However, in recent years, the difficulty and
high cost of developing new drugs, with different acting power, has become a major challenge for
the pharmaceutical industry and the emergence of resistance to drugs already used demonstrates
the enormous need and applicability of screening. of microorganisms for the discovery of new
classes of antimicrobials. Endophytic bacteria are important for the pharmaceutical and
biotechnology field due to the discovery of new substances for human therapeutic use, such as
antibiotics and anticancer drugs. The present study aimed to perform the chemical profile and
evaluation of the antimicrobial activity of bacteria isolated from Phanera splendens. A specimen
of P. splendens was collected at Embrapa Eastern Amazonia in seasonal season and 40
endophytic bacteria were cultured for 7 days in liquid BHI and the liquid medium was liquidliquid
partitioned with AcOEt, the AcOEt extracts had their chemical profiles obtained. HPLC
(High Performance Liquid Chromatography), in which the bacterium B1.5C1 was chosen for
studies, and cultivation was performed in macroscale in solid environment (rice) for 21 days. The
ethanolic solution was liquid-liquid partitioned with EtOAc and hexane. The metabolites were
isolated by chromatographic methods and determined by NMR. The following substances were
identified: fatty acid, triacylglycerol, stigmasterol and β-sitosterol. and identified substances were
evaluated by the MDA and MIC method against Bacillus subtilis (ATCC 6633), Escherichia coli
(ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC
25923) certified bacteria. MDA extracts showed antimicrobial activity and for MIC, fraction
A5C1-5 showed bactericidal activity.
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