Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818

SILVA, Alexandre Vaz da

Artigo

Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818

The present study aimed to isolate and select strains of bacteria with probiotic potential of the intestinal tract of Colossoma macropomum. Fifteen C. macropomum juveniles were reduced to isolate the bacteria, reduced for removal of the digestive tract, then the strains were subjected to in vitro as...

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Autor principal:	SILVA, Alexandre Vaz da
Grau:	Artigo
Publicado em:	2023
Assuntos:	Aquicultura espécies nativas alimento suplementar bactérias patogênicas probiótico Aquaculture native species supplementary food pathogenic bacteria probiotic CNPQ::CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA
Acesso em linha:	https://bdm.ufpa.br:8443/jspui/handle/prefix/5518

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spelling	oai:https:--bdm.ufpa.br:8443:prefix-55182023-04-13T03:05:19Z Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818 SILVA, Alexandre Vaz da CORDEIRO, Carlos Alberto Martins http://lattes.cnpq.br/5010139685215361 SOUSA, Natalino da Costa http://lattes.cnpq.br/3232148110386411 Aquicultura espécies nativas alimento suplementar bactérias patogênicas probiótico Aquaculture native species supplementary food pathogenic bacteria probiotic CNPQ::CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA The present study aimed to isolate and select strains of bacteria with probiotic potential of the intestinal tract of Colossoma macropomum. Fifteen C. macropomum juveniles were reduced to isolate the bacteria, reduced for removal of the digestive tract, then the strains were subjected to in vitro assays, pH and other tests. The experimental design was randomized with different pH variations (4, 5, 6, 8 and 9) and a tube containing 5% (w / v) bile salts. For antagonism tests, four discs (Ø 0.8 cm) were removed and inoculated in TSA culture medium with pathogenic bacteria. Subsequently, as strains with the best results were identified. No total was isolated from 31 bacterial strains of C. macropomum juveniles, with 8 strains selected for in vitro assays. Strain T12A and T4A had a lower reduction in pH 6, 8 and 9 and, for the others, strain T12A decreased 9%. Regarding antagonism, such as T3AR, T4AR, T12A and T2AR strains, they stand out for pointing greater antagonistic action against Gram positive and Gram negative bacteria. Therefore, strain T12A was identified molecularly as Bacillus cereus, demonstrating probiótic potential for Colossoma macropomum (Tambaqui). O presente estudo objetivou isolar e selecionar cepas de bactérias com potencial probiótico do trato intestinal de Colossoma macropomum. Para o isolamento das bactérias foram utilizados quinze juvenis de C. macropomum saudáveis para a retirada do trato digestório, em seguida as cepas foram submetidas a ensaios in vitro, de pH e sais biliares. O delineamento experimental foi inteiramente casualizado com distintas concentrações de pH (4, 5, 6, 8 e 9) e um tubo contendo 5% (p/v) de sais biliares. Para o teste de antagonismo, foram retiradas quatro discos (Ø 0,8 cm) e inoculadas em meio de cultura TSA bactérias patogênicas. Posteriormente, as cepas com os melhores resultados foram identificadas. No total foram isoladas 31 cepas bacterianas dos juvenis de C. macropomum, sendo 8 cepas selecionadas para os ensaios in vitro. A cepa T12A e T4A tiveram menor redução no pH 6, 8 e 9 e para os sais biliares a cepa T12A apresentou redução de 9%. Em relação ao antagonismo, as cepas T3AR, T4AR, T12A e T2AR se destacaram por apontarem maior ação antagônica frente a bactérias Gram positivas e Gram negativas. Portanto, cepa T12A identificada molecularmente como Bacillus cereus, demostrou potencial probiótico para Colossoma macropomum (Tambaqui). 2023-04-12T18:26:09Z 2023-04-12T18:26:09Z 2022-07-01 Trabalho de Conclusão de Curso - Graduação - Artigo SILVA, Alexandre Vaz da; et.al. Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818. In: CONGRESSO BRASILEIRO DE ENGENHARIA DE PESCA, n. 21, 2019, Manaus. Anais eletrônicos [...]. Manaus: CONBEP, 2019. 09 p. Disponível em: https://bdm.ufpa.br:8443/jspui/handle/prefix/5518. Acesso em: . https://bdm.ufpa.br:8443/jspui/handle/prefix/5518 Acesso Aberto
institution	Biblioteca Digital de Monografias - UFPA
collection	MonografiaUFPA
topic	Aquicultura espécies nativas alimento suplementar bactérias patogênicas probiótico Aquaculture native species supplementary food pathogenic bacteria probiotic CNPQ::CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA
spellingShingle	Aquicultura espécies nativas alimento suplementar bactérias patogênicas probiótico Aquaculture native species supplementary food pathogenic bacteria probiotic CNPQ::CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA SILVA, Alexandre Vaz da Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818
topic_facet	Aquicultura espécies nativas alimento suplementar bactérias patogênicas probiótico Aquaculture native species supplementary food pathogenic bacteria probiotic CNPQ::CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA
description	The present study aimed to isolate and select strains of bacteria with probiotic potential of the intestinal tract of Colossoma macropomum. Fifteen C. macropomum juveniles were reduced to isolate the bacteria, reduced for removal of the digestive tract, then the strains were subjected to in vitro assays, pH and other tests. The experimental design was randomized with different pH variations (4, 5, 6, 8 and 9) and a tube containing 5% (w / v) bile salts. For antagonism tests, four discs (Ø 0.8 cm) were removed and inoculated in TSA culture medium with pathogenic bacteria. Subsequently, as strains with the best results were identified. No total was isolated from 31 bacterial strains of C. macropomum juveniles, with 8 strains selected for in vitro assays. Strain T12A and T4A had a lower reduction in pH 6, 8 and 9 and, for the others, strain T12A decreased 9%. Regarding antagonism, such as T3AR, T4AR, T12A and T2AR strains, they stand out for pointing greater antagonistic action against Gram positive and Gram negative bacteria. Therefore, strain T12A was identified molecularly as Bacillus cereus, demonstrating probiótic potential for Colossoma macropomum (Tambaqui).
author_additional	CORDEIRO, Carlos Alberto Martins
author_additionalStr	CORDEIRO, Carlos Alberto Martins
format	Artigo
author	SILVA, Alexandre Vaz da
title	Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818
title_short	Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818
title_full	Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818
title_fullStr	Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818
title_full_unstemmed	Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818
title_sort	seleção e desafio in vitro de bactérias probióticas isoladas do intestino do tambaqui colossoma macropomum cuvier, 1818
publishDate	2023
url	https://bdm.ufpa.br:8443/jspui/handle/prefix/5518
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score	11.755432

Seleção e desafio in vitro de bactérias probióticas isoladas do intestino do Tambaqui Colossoma macropomum Cuvier, 1818

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