Introduction: The incorporation of natural products into oral hygiene articles have benefits, such as antimicrobial activity, as well as the reduction of side effects. However, before these products reach the market, it is necessary to perform laboratory tests that analyze possible changes that may cause adverse reactions in individuals. Objective: To evaluate comparatively the in vitro cytotoxic potential of mouthwashes available on the market and experimental rinses with Amazonian vegetable oils. Material and method: For the test, human periodontal ligament fibroblasts, seeded at a concentration of 1x104 cells / well, were exposed for 1 minute to test solutions containing 200μL of mouthwashes formulated with the association of vegetable oils of Astrocaryum vulgare and Bactris gasipae concentrations of 5% and 10% and commercial mouthwashes. As a control, culture medium was used. Cytotoxicity was assessed by the methyltetrazolium test and the count performed on the Elisa absorbance reader. The test was performed in triplicate and the data analyzed with the ANOVA test followed by Tukey (α = 0.05). Results: Commercial mouthwashes showed statistical similarity (p> 0.05) and high cytotoxicity (32-38% of cell viability). Experimental mouthwashes presented 60-75% of viable fibroblasts, with no difference between the concentrations evaluated and the control (p> 0.05). Conclusion: The experimental products developed with vegetable oils were less harmful to cells when compared to commercial products, and could be an alternative for the control of dental biofilm, with a lower risk of adverse effects.
|
