Human being has the need to mix water with insoluble substances in their daily and industrial
activities. Surfactants derived from petroleum have been used for this purpose, however, they
have problems such as their toxicity and biodegradability. Biosurfactants from microbial
origin are an alternative to this, on the other hand, its production cost is high due to the
substrates that are used in the bioprocesses. In this context, the aim of this study was to
investigate the use of wastes as a substrate for the production of biosurfactants by filamentous
fungi isolated from soil samples in Amazon region. Bioprocess were conducted with five
known fungi isolates producers of biosurfactants in order to investigate which of them would
produce in a shorter time. Then, we evaluated the possibility using wastes of peel from
tucumã, peach palm, banana, cupuacu and also the post-fry oil as substrate for the bioprocess
with the selected isolated. Factors that influence the production of biosurfactant were
evaluated by factorial design. As a result, the fungus Fusarium oxysporum 87-2LIVI
produced high levels of biosurfactants in three days of bioprocess. Moreover, this specie also
showed highlight due to high biosurfactant production using peach palm peel as substract.
The pH and yeast extract were the most important factors related to production of
biosurfactant by this isolated. Thus, the present study showed isolated of F. oxysporum specie
from Amazonian soil is potential producer of surfactants and also Amazon waste, particularly,
peel of peach palm has the potential to be used as a bioprocess substrate for production of
biosurfactant.
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