Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT
Arrabidaea Chica (Bigonnnace) is commonly used in The Amazon by folks due to its anti-inflammatory properties, properties that have been confirmed in different experimental models of disease, but the molecular processes that govern this effect were unclear. The immune system mediates inflammation...
| Autor principal: | Ubiera, Rosmery Duran |
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| Outros Autores: | http://lattes.cnpq.br/6310867371281249, https://orcid.org/0000-0002-6764-245X |
| Grau: | Tese |
| Idioma: | eng |
| Publicado em: |
Universidade Federal do Amazonas
2021
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| Acesso em linha: |
https://tede.ufam.edu.br/handle/tede/8399 |
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oai:https:--tede.ufam.edu.br-handle-:tede-8399 |
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oai:https:--tede.ufam.edu.br-handle-:tede-83992021-08-18T05:03:34Z Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT Efeitos anti-inflamatórios dependentes de adenosina de Arrabidaea Chica VERLOT Ubiera, Rosmery Duran Alves Filho, José Carlos Farias http://lattes.cnpq.br/6310867371281249 http://lattes.cnpq.br/1316491658346452 Sadahiro, Aya http://lattes.cnpq.br/8658798733544812 Corrêa, José Wilson do Nascimento http://lattes.cnpq.br/9130747433263550 Silva, Rafael Brito da http://lattes.cnpq.br/5279568988466735 https://orcid.org/0000-0002-6764-245X Propriedades anti-inflamatórias Citocina IL-1β Macrófagos derivados da medula óssea Adenosina Desfosforilação de ATP CIÊNCIAS BIOLÓGICAS Arrabidaea Chica VERLOT Inflammation Anti-inflammatory Adenosine ATP IL-10 IL-1β Arrabidaea Chica (Bigonnnace) is commonly used in The Amazon by folks due to its anti-inflammatory properties, properties that have been confirmed in different experimental models of disease, but the molecular processes that govern this effect were unclear. The immune system mediates inflammation through a variety of mechanisms, including the production of pro-inflammatory and anti-inflammatory cytokines, which are directed by different signaling pathways, among them the adenosinergic pathway. The study of new antiinflammatory molecules provides a long-term solution to the global problem that represents inflammation when uncontrolled and the Amazon is rich in natural products that need to be studied. The objective of this study was to elucidate whether or not the anti-inflammatory effect of Arrabidaea Chica VERLOT in bone marrow-derived macrophages (BMDM) is mediated by adenosine. The production of pro-inflammatory (IL-1β and TNFα) and anti-inflammatory (IL-10) cytokines, was assessed by ELISA in the supernatant of BMDM preincubated with Arrabidaea ChicaVERLOT aqueous extract and stimulated with LPS. Arrabidaea Chica demonstrated to decrease the production of IL-1β and increase the production of IL-10. The increased production of IL-10 was associated with elevated production of adenosine and this effect was not observed when the different steps of the adenosinergic pathway were pharmacologically inhibited or genetically suppressed. The participation of the metabolism of the cells was also confirmed as the direct source of ATP for the generation of adenosine. We conclude that Arrabidaea Chica VERLOT mediates its anti-inflammatory effect via the adenosinergic pathway, increasing the metabolic rate of the cells to generate the substrate for this pathway. Arrabidaea Chica (Bigonnnace) is commonly used in The Amazon by folks due to its anti-inflammatory properties, properties that have been confirmed in different experimental models of disease, but the molecular processes that govern this effect were unclear. The immune system mediates inflammation through a variety of mechanisms, including the production of pro-inflammatory and anti-inflammatory cytokines, which are directed by different signaling pathways, among them the adenosinergic pathway. The study of new antiinflammatory molecules provides a long-term solution to the global problem that represents inflammation when uncontrolled and the Amazon is rich in natural products that need to be studied. The objective of this study was to elucidate whether or not the anti-inflammatory effect of Arrabidaea Chica VERLOT in bone marrow-derived macrophages (BMDM) is mediated by adenosine. The production of pro-inflammatory (IL-1β and TNFα) and anti-inflammatory (IL-10) cytokines, was assessed by ELISA in the supernatant of BMDM preincubated with Arrabidaea ChicaVERLOT aqueous extract and stimulated with LPS. Arrabidaea Chica demonstrated to decrease the production of IL-1β and increase the production of IL-10. The increased production of IL-10 was associated with elevated production of adenosine and this effect was not observed when the different steps of the adenosinergic pathway were pharmacologically inhibited or genetically suppressed. The participation of the metabolism of the cells was also confirmed as the direct source of ATP for the generation of adenosine. We conclude that Arrabidaea Chica VERLOT mediates its anti-inflammatory effect via the adenosinergic pathway, increasing the metabolic rate of the cells to generate the substrate for this pathway. 2021-08-17T16:02:09Z 2021-04-14 Tese UBIERA, Rosmery Duran. Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT. 2021. 80 f. Tese (Doutorado em Imunologia Básica e Aplicada) - Universidade Federal do Amazonas, Manaus, 2021. https://tede.ufam.edu.br/handle/tede/8399 eng Acesso Aberto http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Universidade Federal do Amazonas Instituto de Ciências Biológicas Brasil UFAM Programa de Pós-graduação em Imunologia Básica e Aplicada |
| institution |
TEDE - Universidade Federal do Amazonas |
| collection |
TEDE-UFAM |
| language |
eng |
| topic |
Propriedades anti-inflamatórias Citocina IL-1β Macrófagos derivados da medula óssea Adenosina Desfosforilação de ATP CIÊNCIAS BIOLÓGICAS Arrabidaea Chica VERLOT Inflammation Anti-inflammatory Adenosine ATP IL-10 IL-1β |
| spellingShingle |
Propriedades anti-inflamatórias Citocina IL-1β Macrófagos derivados da medula óssea Adenosina Desfosforilação de ATP CIÊNCIAS BIOLÓGICAS Arrabidaea Chica VERLOT Inflammation Anti-inflammatory Adenosine ATP IL-10 IL-1β Ubiera, Rosmery Duran Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
| topic_facet |
Propriedades anti-inflamatórias Citocina IL-1β Macrófagos derivados da medula óssea Adenosina Desfosforilação de ATP CIÊNCIAS BIOLÓGICAS Arrabidaea Chica VERLOT Inflammation Anti-inflammatory Adenosine ATP IL-10 IL-1β |
| description |
Arrabidaea Chica (Bigonnnace) is commonly used in The Amazon by folks due to its anti-inflammatory properties, properties that have been confirmed in different experimental
models of disease, but the molecular processes that govern this effect were unclear. The
immune system mediates inflammation through a variety of mechanisms, including the
production of pro-inflammatory and anti-inflammatory cytokines, which are directed by
different signaling pathways, among them the adenosinergic pathway. The study of new antiinflammatory molecules provides a long-term solution to the global problem that represents
inflammation when uncontrolled and the Amazon is rich in natural products that need to be
studied. The objective of this study was to elucidate whether or not the anti-inflammatory
effect of Arrabidaea Chica VERLOT in bone marrow-derived macrophages (BMDM) is
mediated by adenosine. The production of pro-inflammatory (IL-1β and TNFα) and anti-inflammatory (IL-10) cytokines, was assessed by ELISA in the supernatant of BMDM preincubated with Arrabidaea ChicaVERLOT aqueous extract and stimulated with LPS.
Arrabidaea Chica demonstrated to decrease the production of IL-1β and increase the
production of IL-10. The increased production of IL-10 was associated with elevated
production of adenosine and this effect was not observed when the different steps of the
adenosinergic pathway were pharmacologically inhibited or genetically suppressed. The
participation of the metabolism of the cells was also confirmed as the direct source of ATP
for the generation of adenosine. We conclude that Arrabidaea Chica VERLOT mediates its
anti-inflammatory effect via the adenosinergic pathway, increasing the metabolic rate of the
cells to generate the substrate for this pathway. |
| author_additional |
Alves Filho, José Carlos Farias |
| author_additionalStr |
Alves Filho, José Carlos Farias |
| format |
Tese |
| author |
Ubiera, Rosmery Duran |
| author2 |
http://lattes.cnpq.br/6310867371281249 https://orcid.org/0000-0002-6764-245X |
| author2Str |
http://lattes.cnpq.br/6310867371281249 https://orcid.org/0000-0002-6764-245X |
| title |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
| title_short |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
| title_full |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
| title_fullStr |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
| title_full_unstemmed |
Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT |
| title_sort |
adenosine dependent anti-inflammatory effects of arrabidaea chica verlot |
| publisher |
Universidade Federal do Amazonas |
| publishDate |
2021 |
| url |
https://tede.ufam.edu.br/handle/tede/8399 |
| _version_ |
1831969977776734208 |
| score |
11.755432 |