Perfil fenotípico e genotípico de uma cepa de Escherichia coli multirresistente a antibióticos, isolada do lago Água Preta, Belém, Pará.
The present work aimed to describe the phenotypic and genotypic profile of a bacterium strain resistant to last resort antibiotic, isolated from the Água Preta lake, water supply source in Belém, Pará. The phenotypic characterization methods were Gram staining, direct microscopy and in vitro sens...
| Autor principal: | LIMA, Alyne Cristina Sodré |
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| Outros Autores: | CASTRO, Ícaro Rainyer Rodrigues de, ALVES, Jorianne Thyeska Castro, MOURA, Vitória Almeida Gonçalves de, PAIXÃO, Carla Thais Moreira, COSTA, Wana Lailan Oliveira da, ALCÂNTARA, Adriedson Jameson Chaves de, ARAÚJO, Carlos Leonardo de Aragão, DIAS, Larissa Maranhão, SILVA, Artur Luiz da Costa da, FOLADOR, Adriana Ribeiro Carneiro |
| Grau: | Capítulo de Livro |
| Idioma: | por |
| Publicado em: |
Instituto Federal do Amapá
2021
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| Assuntos: | |
| Acesso em linha: |
http://repositorio.ifap.edu.br:8080/jspui/handle/prefix/410 |
| Resumo: |
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The present work aimed to describe the phenotypic and genotypic profile
of a bacterium strain resistant to last resort antibiotic, isolated from the Água Preta
lake, water supply source in Belém, Pará. The phenotypic characterization methods
were Gram staining, direct microscopy and in vitro sensitivity test. For genotypic
characterization purposes, genomic DNA extraction, species differentiation through
Quadruplex PCR and PCR for identification of resistance genes were performed.
The C40A strain was identified as an Escherichia coli belonging to filogroup D. The
bacterium was resistant to 87.5% of the clinically available antibiotics for the treatment
of bacterial infections and susceptible to only 12.5% (Imipenem and Kanamycin). The
presence of the blaCTX gene and the mobile elements Intl1 and Intl2 were identified.
These genetic components were markedly associated with the expression of antibiotic
resistance profiles. |