Perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de Boa Vista - RR
One of the main concerns of humanity nowadays is water: its availability and its quality. An inadequate exploitation of the sources results in the contamination of both superficial and deep waters, which become a public health hazard. Bacteria are one of these biological sources of water contaminati...
| Autor principal: | Pessoa, Ilzo Costa |
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| Grau: | Dissertação |
| Idioma: | por |
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Universidade Federal de Roraima
2020
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http://repositorio.ufrr.br:8080/jspui/handle/prefix/314 |
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oai:repositorio.ufrr.br:prefix-3142024-01-04T15:06:27Z Perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de Boa Vista - RR Resistance to antimicrobians of isolated bacteria from water samples collected in state school of boa Vista - RR Pessoa, Ilzo Costa Vital, Marcos José Salgado http://lattes.cnpq.br/7855596789769104 Qualidade da água Bactérias heterotróficas Coliformes Antimicrobianos Water quality Heterotrophic bacteria Coliforms Antimicrobials CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL One of the main concerns of humanity nowadays is water: its availability and its quality. An inadequate exploitation of the sources results in the contamination of both superficial and deep waters, which become a public health hazard. Bacteria are one of these biological sources of water contamination, responsible for countless diseases which are treated with antimicrobials. The widely spread use of antimicrobials in human medicine along with animal production had resulted in a steady increase in the number of pathogenic and commensal bacteria resistant to antimicrobial agents, which in recent years are becoming one of the main problems of public health. This paper is a result of an interest in studying the quality of water consumed by the students of 20 state schools of the public school system of Boa Vista – RR, as well as trying to obtain a profile of antimicrobial resistance of the bacteria isolated in different water samples collected at these schools. Between October 2010 and April 2011, 40 water samples were collected from taps in lunchrooms, along with 40 samples from drinking wells, and all of them were submitted to microbiological examination. Using the multiple tubes technique, with 3 dilutions and a series of 5 tubes, we determined the total number of coliforms and thermotolerant coliforms. In agreement with the rules of the Standard Methods for the Examination of Water and Wastewater, the “pour plate” method was used at the CBio – UFRR to detect heterotrophic bacteria. Isolation and identification of microorganisms was done at LACEN – RR, using the biochemical series. In evaluating the resistance to antimicrobials, disk diffusion methods were used, in agreement with the established rules of the CLSI. In water samples that receive full treatment, 10% tested positive for total coliforms and, in chlorinated water samples, 20% tested positive for total coliforms and 2,5% for thermo-tolerant coliforms, which makes them inadequate for human consume. The counting of heterotrophic bacteria went from 1,4 x 10 to 3,6 x 10² CFU/mL of water, not surpassing what is established in the ordinance 518/2004/MS. The following Gram-negative bacteria (66,7%) were identified: Acinetobacter spp., C. violacea, Cocobacilos spp., E. aerogenes, E. coli, K. ozaenae, Leptothrix ssp. and Gram-negative glucose non fermenting bacillus. The Gram-positive bacteria identified were: B. subtilis, Micrococcus spp., S. aureus and Staphylococcus spp. The results of antibiotic sensibility tests revealed that most of the isolated bacteria were resistant to ampicilin (68,2%), 22,3% showed resistance to oxacilin and 22,7% were resistant to both eritromicin and meropenem. In Gram-positive bacteria, the growths of S. aureus isolated from water samples showed the greatest MAR index (0,61), while E. coli growths were the ones who showed the greatest MAR index in Gram-negative bacteria (also 6,1%). Results prove a great instability in the quality of water provided to the Boa Vista - RR population, being in its treatment, distribution or containment, as well as showing the presence of bacteria resistant to multiple antibiotics, which is a health hazard to the public. Atualmente, uma das maiores preocupações da humanidade é com a disponibilidade e qualidade da água. A exploração inadequada das fontes acarreta a contaminação das águas superficiais e subterrâneas que se tornam um risco para a saúde pública. Dentre as fontes biológicas de contaminação da água, incluem-se as bactérias, que são responsáveis por inúmeras doenças, sendo frequentemente tratadas com antimicrobianos. O amplo uso de antimicrobianos na medicina humana e na produção animal tem resultado no aumento do número de bactérias comensais e patogênicas resistentes a agentes antimicrobianos, fato que nos últimos anos vem se tornando um dos principais problemas de saúde pública. O presente trabalho resulta do interesse pelo estudo da qualidade da água consumida pelos alunos de 20 escolas estaduais da rede pública de Boa Vista – RR, bem como obter o perfil de resistência a antimicrobianos de bactérias isoladas a partir de diferentes amostras de águas, coletadas nessas escolas. No período de outubro de 2010 a abril de 2011, foram coletas 40 amostras de águas de torneiras das copas e 40 amostras de águas de bebedouros, as quais foram submetidas à avaliação microbiológica. A determinação dos coliformes totais e termotolerantes foi realizada por meio da técnica dos tubos múltiplos com 3 diluições e série de 5 tubos. Para a detecção de bactérias heterotróficas uso-se o método ”pour plate”, de acordo com as normas preconizadas no Standard Methods for the Examination of Water and Wastwater. O isolamento e a identificação dos isolados bacteriano foi realizada no LACEN-RR utilizando-se a série bioquímica. Para a avaliação da resistência aos antimicrobianos, utilizou-se a metodologia de difusão de disco, seguindo as normas estabelecidas pelo CLSI. Das amostras de águas que recebem tratamento completo, 10% apresentaram resultados positivos para coliformes totais e nas amostras de água clorada, 20% apresentaram resultados positivos para coliformes totais e 2,5% resultado positivo para coliformes termotolerantes, o que as tornam imprópria para o consumo humano. A contagem de bactérias heterotróficas variou de 1,4 x 10 a 3,6 x 102 UFC/mL de água, não ultrapassando o estabelecido pela Portaria 518/2004/MS. Foram identificadas as seguintes bactérias Gram-negativas (66,7%): Acinetobacter spp., C. violacea, Cocobacilos spp., E. aerogenes, E. coli, K. ozaenae, Leptothrix ssp. e Bacilo Gram-negativo não fermentador de glicose, as bactérias Gram-positivas (33,3%), identificadas foram: B. subtilis, Micrococcus spp., S. aureus e Staphylococcus spp. Os resultados dos testes de sensibilidade aos antibióticos revelaram que a maioria das bactérias isoladas foi resistente a ampicilina (68,2%), 27,3% apresentaram resistência a oxacilina e 22,7% foram resistentes a eritromicina e meropenem. As cepas de S. aureus isoladas das amostras de águas apresentaram maior índice MAR (0,61) entre as bactérias Gram-positivas, enquanto que nas bactérias Gram-negativas, as cepas que apresentaram maior índice MAR foram as cepas de E. coli, com índice 0,61. Os resultados comprovam a ocorrência de instabilidade no padrão de qualidade da água fornecida a população de Boa Vista – RR seja no tratamento, na distribuição ou no armazenamento da água. Além de constatar a presença de bactérias multirresistentes a antibióticos, o que representa risco à população. 2020-06-15T21:48:11Z 2020-06-15T21:48:11Z 2011 Dissertação PESSOA, Ilzo Costa. Perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de Boa Vista - RR. 2011. 98f. Dissertação (Mestrado em Ciências Ambientais) - Programa de Pós-Graduação em Recursos Naturais, Universidade Federal de Roraima, Boa Vista, 2011. http://repositorio.ufrr.br:8080/jspui/handle/prefix/314 por Acesso Aberto Universidade Federal de Roraima Brasil PRPPG - Pró-Reitoria de Pesquisa e Pós-Graduação PRONAT - Programa de Pós-Graduação em Recursos Naturais UFRR |
| institution |
Repositório Institucional - Universidade Federa de Roraima - UFRR |
| collection |
DSpace |
| language |
por |
| topic |
Qualidade da água Bactérias heterotróficas Coliformes Antimicrobianos Water quality Heterotrophic bacteria Coliforms Antimicrobials CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
| spellingShingle |
Qualidade da água Bactérias heterotróficas Coliformes Antimicrobianos Water quality Heterotrophic bacteria Coliforms Antimicrobials CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL Pessoa, Ilzo Costa Perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de Boa Vista - RR |
| topic_facet |
Qualidade da água Bactérias heterotróficas Coliformes Antimicrobianos Water quality Heterotrophic bacteria Coliforms Antimicrobials CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
| description |
One of the main concerns of humanity nowadays is water: its availability and its quality. An inadequate exploitation of the sources results in the contamination of both superficial and deep waters, which become a public health hazard. Bacteria are one of these biological sources of water contamination, responsible for countless diseases which are treated with antimicrobials. The widely spread use of antimicrobials in human medicine along with animal production had resulted in a steady increase in the number of pathogenic and commensal bacteria resistant to antimicrobial agents, which in recent years are becoming one of the main problems of public health. This paper is a result of an interest in studying the quality of water consumed by the students of 20 state schools of the public school system of Boa Vista – RR, as well as trying to obtain a profile of antimicrobial resistance of the bacteria isolated in different water samples collected at these schools. Between October 2010 and April 2011, 40 water samples were collected from taps in lunchrooms, along with 40 samples from drinking wells, and all of them were submitted to microbiological examination. Using the multiple tubes technique, with 3 dilutions and a series of 5 tubes, we determined the total number of coliforms and thermotolerant coliforms. In agreement with the rules of the Standard Methods for the Examination of Water and Wastewater, the “pour plate” method was used at the CBio – UFRR to detect heterotrophic bacteria. Isolation and identification of microorganisms was done at LACEN – RR, using the biochemical series. In evaluating the resistance to antimicrobials, disk diffusion methods were used, in agreement with the established rules of the CLSI. In water samples that receive full treatment, 10% tested positive for total coliforms and, in chlorinated water samples, 20% tested positive for total coliforms and 2,5% for thermo-tolerant coliforms, which makes them inadequate for human consume. The counting of heterotrophic bacteria went from 1,4 x 10 to 3,6 x 10² CFU/mL of water, not surpassing what is established in the ordinance 518/2004/MS. The following Gram-negative bacteria (66,7%) were identified: Acinetobacter spp., C. violacea, Cocobacilos spp., E. aerogenes, E. coli, K. ozaenae, Leptothrix ssp. and Gram-negative glucose non fermenting bacillus. The Gram-positive bacteria identified were: B. subtilis, Micrococcus spp., S. aureus and Staphylococcus spp. The results of antibiotic sensibility tests revealed that most of the isolated bacteria were resistant to ampicilin (68,2%), 22,3% showed resistance to oxacilin and 22,7% were resistant to both eritromicin and meropenem. In Gram-positive bacteria, the growths of S. aureus isolated from water samples showed the greatest MAR index (0,61), while E. coli growths were the ones who showed the greatest MAR index in Gram-negative bacteria (also 6,1%). Results prove a great instability in the quality of water provided to the Boa Vista - RR population, being in its treatment, distribution or containment, as well as showing the presence of bacteria resistant to multiple antibiotics, which is a health hazard to the public. |
| author_additional |
Vital, Marcos José Salgado |
| author_additionalStr |
Vital, Marcos José Salgado |
| format |
Dissertação |
| author |
Pessoa, Ilzo Costa |
| title |
Perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de Boa Vista - RR |
| title_short |
Perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de Boa Vista - RR |
| title_full |
Perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de Boa Vista - RR |
| title_fullStr |
Perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de Boa Vista - RR |
| title_full_unstemmed |
Perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de Boa Vista - RR |
| title_sort |
perfil de resistência a antimicrobianos de bactérias isoladas de amostras de águas das escolas estaduais de boa vista - rr |
| publisher |
Universidade Federal de Roraima |
| publishDate |
2020 |
| url |
http://repositorio.ufrr.br:8080/jspui/handle/prefix/314 |
| _version_ |
1787240123257061376 |
| score |
11.653393 |