Identificação e diferenciação da expressão de genes em Tambaqui (Colossoma macropomum CUVIER, 1818) alimentado com frutos e sementes da Amazônia
Fruits play an important role in the Central Amazonia floodplain ecosystem, especially when considering the relation fish-plants. More than 110 plants species have been identified which produc fruits and seeds consumed by tambaqui in its natural environment. The knowledge of the food habits of th...
| Autor principal: | Añez, Luciana Melhorança Moreira |
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| Grau: | Tese |
| Idioma: | por |
| Publicado em: |
Instituto Nacional de Pesquisas da Amazônia - INPA
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/12253 http://lattes.cnpq.br/3410008163216423 |
| Resumo: |
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Fruits play an important role in the Central Amazonia floodplain ecosystem, especially
when considering the relation fish-plants. More than 110 plants species have been
identified which produc fruits and seeds consumed by tambaqui in its natural
environment. The knowledge of the food habits of these animals in nature, made
possible the inclusion of fruits and seeds in the fish diet created when reared under
captivity, aiming to decrease production costs, and keeping a satisfactory fish growth
rate. However, the presence of antinutritional factors can limit the inclusion of certain
vegetable products in the diet of these fishes. This work aims to evaluate, at a molecular
level, the effect of the incorporation of the fruits ‘camu-camu’, ‘catoré’, ‘embaúba’,
‘jauari’ and ‘munguba’ seeds in tambaqui feeding, in the ratio of 1:1 with a commercial
ration. The physiological, biochemical and cytogenotoxicity parameters were also
evaluated for their effects in the fishes submitted to a period of 1, 15 and 30 days of
modified feeding. The results demonstrated that fruits and seeds did not affect
metabolic, enzymatic and cytogenotoxic alterations in the fishes during the tested
periods. However, it was possible to verify a significant difference in the feeding period
and food deprivation in subbimetted fishes. The gene differential expression showded
fragments that presented similarity with Adenylate Kinase, Superoxide Dismutase,
Apolipoprotein and Cytochrome P450 enziyme genes. Others seven expressed
transcripts did not demonstrated similarity with enzymes deposited in the GenBank, and
two fragments were characterized as products of proteins not identified thefore being
candidates for future studies in order to understand the animals process metabolism
when fed with supplemented rations with Amazonian fruits and seeds. |