Efeito da temperatura no desenvolvimento de imaturos de Paralucilia fulvinota (Bigot, 1877) (Diptera: Calliphoridae) de interesse forense
Forensic entomology uses information based on the biology of some insects, mainly Diptera Calliphoridae, which are associated to corpses in forensic investigations. Temperature has direct effect on the metabolism rate. Higher temperatures reduce the development time and has a direct effect on the...
| Autor principal: | Sales, Tohnson |
|---|---|
| Grau: | Dissertação |
| Idioma: | por |
| Publicado em: |
Instituto Nacional de Pesquisas da Amazônia - INPA
2020
|
| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/12489 http://lattes.cnpq.br/8599621096681727 |
| id |
oai:repositorio:1-12489 |
|---|---|
| recordtype |
dspace |
| spelling |
oai:repositorio:1-12489 Efeito da temperatura no desenvolvimento de imaturos de Paralucilia fulvinota (Bigot, 1877) (Diptera: Calliphoridae) de interesse forense Sales, Tohnson Keppler, Ruth Leila Ferreira Martins, Renato Tavares Paralucilia fulvinota Entomologia forense Decomposição Forensic entomology uses information based on the biology of some insects, mainly Diptera Calliphoridae, which are associated to corpses in forensic investigations. Temperature has direct effect on the metabolism rate. Higher temperatures reduce the development time and has a direct effect on the body mass and length. This research aims at studying the effects of ambient temperature (average temp. =24,5 ± 1,3ºC; average humidity 84 ± 3,9%), in 5 different rates of controlled temperature (20, 23, 26, 29, 32 and 35 ºC), on the development of P. fulvinota, thus generating data to help estimate the PMI (Postmortem Interval) of 5 corpses found in this area. The research was conducted at Reserva Florestal Ducke (Manaus-Amazonas). Five pregnant female mosquitoes were collected when feeding upon a forty-kilo swine (Sus scrofa). After being laid, immatures were collected from stock colonies. In ambient temperature, the individuals development took 13 days. Gender ratio was 0,86 (7 ♂ and 46 ♀). There was a constant mass gain through each instar phase in relation to the larvae length. The data in degree day (190.38) and degree hour (4569.24) of the total development time from larvae (L1, L2, L3) to adult reveal the elapsed time to reach the total period. Development time under controlled temperature was longer in 20º C (~25 days) with a gender ratio of 0,3 (7 ♂ and 3 ♀), whereas the shorter was in 29º C (~11 days). Gender ratio: 0,6 (18 ♂ and 27 ♀). At 23º C, 19 days, gender ratio: 0,45 (13 ♂ e 11 ♀) and 26º C, 13 days, gender ratio: 0, 5 (21 ♂ e 21 ♀). At 23º and 26º C there was a consistent length growth, unlikely the other temperatures where the growth rate was decreased. Regarding body mass there was gain in all temperatures apart from 29º C. At 32º and 35º C incomplete development occurred. Under controlled temperatures the optimal temperatures ranged between 23 and 26º C and in fluctuating temperatures, average 24, 5º C. In order to obtain an accurate result using PMI calculation, results from experiments under controlled and ambient temperatures were used and complemented each other, confirming the temperature influence on the development time, body mass and length. This study represents an important tool to forensic entomology and the PMI estimation in corpses found in this region. A Entomologia Forense utiliza informações sobre a biologia dos insetos, principalmente os Diptera Calliphoridae, associados a cadáveres em investigações científicas. A temperatura possui influência na taxa metabólica dos insetos. Geralmente as mais elevadas diminuem o tempo de desenvolvimento e afetam o comprimento e massa corpórea. Este trabalho buscou estudar o efeito da temperatura ambiente (média =24,5 ± 1,3ºC; e umidade média de 84 ± 3,9%) e em cinco faixas de temperatura controlada (20, 23, 26, 29, 32 e 35 ºC) no desenvolvimento de P. fulvinota, fornecendo dados para auxiliar na estimativa do IPM (Intervalo pós-morte) de cadáveres encontrados nesta região. O estudo foi realizado de outubro a dezembro de 2014 na Reserva Florestal Ducke (Manaus-Amazonas). Fêmeas grávidas foram coletadas em um suíno (Sus scrofa) de 40 Kg e a partir das posturas foram obtidos os imaturos provenientes de colônias de estoque. O tempo de desenvolvimento em temperatura ambiente foi de 13,13 dias com razão sexual de 0,86 (7 ♂ e 46 ♀). Houve aumento constante do ganho de massa ao longo de cada instar em relaçao ao comprimento larval. Dados de Grausdias (190.38) e Graus-horas (4569.24) do tempo total de larva (L1, L2,L3) a adulto, mostram o tempo transcorrido para atingir o período total. O tempo desenvolvimento em temperatura controlada foi maior a 20º C (~25 dias) com razão sexual de 0,3 (7 ♂ e 3 ♀). O menor a 29º C (~11dias) com razão sexual de 0,6 (18 ♂ e 27 ♀). A 23º C foi de 19 dias com razão sexual de 0,45 (13 ♂ e 11 ♀ ) e a 26º C foi de 13 dias com razão sexual de 0, 5 (21 ♂ e 21 ♀). Nas temperaturas de 23º e 26º C houve ganho constante de comprimento enquanto nas demais esse ganho diminuiu. Em relação a massa houve ganho constante, com execessão da temperatura de 29º C. Nas temperaturas de 32 e 35º C não houve desenvolvimento completo. Em temperaturas controladas o limiar ótimo esteve entre 23 e 26º C e em temperatura flutuante em média de 24, 5º C. Para que se possa obter um resultado mais preciso para o calculo do IPM, ambos resultados (temperatura controlada e ambiente) se complementam, confirmando a influencia da temperatura sobre o tempo de desenvolvimento, massa e comprimento. Essa análise se constitui importante ferramenta para entomologia forense e determinação do Intervalo pós-morte (IPM). em cadáveres encontrados na região. 2020-02-17T20:21:53Z 2020-02-17T20:21:53Z 2015-04-30 Dissertação https://repositorio.inpa.gov.br/handle/1/12489 http://lattes.cnpq.br/8599621096681727 por Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Instituto Nacional de Pesquisas da Amazônia - INPA Entomologia |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
por |
| topic |
Paralucilia fulvinota Entomologia forense Decomposição |
| spellingShingle |
Paralucilia fulvinota Entomologia forense Decomposição Sales, Tohnson Efeito da temperatura no desenvolvimento de imaturos de Paralucilia fulvinota (Bigot, 1877) (Diptera: Calliphoridae) de interesse forense |
| topic_facet |
Paralucilia fulvinota Entomologia forense Decomposição |
| description |
Forensic entomology uses information based on the biology of some insects, mainly
Diptera Calliphoridae, which are associated to corpses in forensic investigations.
Temperature has direct effect on the metabolism rate. Higher temperatures reduce the
development time and has a direct effect on the body mass and length. This research
aims at studying the effects of ambient temperature (average temp. =24,5 ± 1,3ºC;
average humidity 84 ± 3,9%), in 5 different rates of controlled temperature (20, 23, 26,
29, 32 and 35 ºC), on the development of P. fulvinota, thus generating data to help
estimate the PMI (Postmortem Interval) of 5 corpses found in this area. The research
was conducted at Reserva Florestal Ducke (Manaus-Amazonas). Five pregnant female
mosquitoes were collected when feeding upon a forty-kilo swine (Sus scrofa). After
being laid, immatures were collected from stock colonies. In ambient temperature, the
individuals development took 13 days. Gender ratio was 0,86 (7 ♂ and 46 ♀). There
was a constant mass gain through each instar phase in relation to the larvae length. The
data in degree day (190.38) and degree hour (4569.24) of the total development time
from larvae (L1, L2, L3) to adult reveal the elapsed time to reach the total period.
Development time under controlled temperature was longer in 20º C (~25 days) with a
gender ratio of 0,3 (7 ♂ and 3 ♀), whereas the shorter was in 29º C (~11 days). Gender
ratio: 0,6 (18 ♂ and 27 ♀). At 23º C, 19 days, gender ratio: 0,45 (13 ♂ e 11 ♀) and 26º
C, 13 days, gender ratio: 0, 5 (21 ♂ e 21 ♀). At 23º and 26º C there was a consistent
length growth, unlikely the other temperatures where the growth rate was decreased.
Regarding body mass there was gain in all temperatures apart from 29º C. At 32º and
35º C incomplete development occurred. Under controlled temperatures the optimal
temperatures ranged between 23 and 26º C and in fluctuating temperatures, average 24,
5º C. In order to obtain an accurate result using PMI calculation, results from
experiments under controlled and ambient temperatures were used and complemented
each other, confirming the temperature influence on the development time, body mass
and length. This study represents an important tool to forensic entomology and the PMI
estimation in corpses found in this region. |
| author_additional |
Keppler, Ruth Leila Ferreira |
| author_additionalStr |
Keppler, Ruth Leila Ferreira |
| format |
Dissertação |
| author |
Sales, Tohnson |
| title |
Efeito da temperatura no desenvolvimento de imaturos de Paralucilia fulvinota (Bigot, 1877) (Diptera: Calliphoridae) de interesse forense |
| title_short |
Efeito da temperatura no desenvolvimento de imaturos de Paralucilia fulvinota (Bigot, 1877) (Diptera: Calliphoridae) de interesse forense |
| title_full |
Efeito da temperatura no desenvolvimento de imaturos de Paralucilia fulvinota (Bigot, 1877) (Diptera: Calliphoridae) de interesse forense |
| title_fullStr |
Efeito da temperatura no desenvolvimento de imaturos de Paralucilia fulvinota (Bigot, 1877) (Diptera: Calliphoridae) de interesse forense |
| title_full_unstemmed |
Efeito da temperatura no desenvolvimento de imaturos de Paralucilia fulvinota (Bigot, 1877) (Diptera: Calliphoridae) de interesse forense |
| title_sort |
efeito da temperatura no desenvolvimento de imaturos de paralucilia fulvinota (bigot, 1877) (diptera: calliphoridae) de interesse forense |
| publisher |
Instituto Nacional de Pesquisas da Amazônia - INPA |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/12489 http://lattes.cnpq.br/8599621096681727 |
| _version_ |
1787143299520266240 |
| score |
11.755432 |