In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood

Oliveira, Viviana Maria Araújo de; Carneiro, Ana Lúcia Basílio; Caúper, Gláucia Socorro de Barros; Pohlit, Adrian Martin

Artigo

In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood

In the present study, different aerial parts from twelve Amazonian plant species found in the National Institute for Amazon Research's (INPA's) Adolpho Ducke Forest Reserve (in Manaus, Amazonas, Brazil) were collected. Separate portions of dried, ground plant materials were extracted with water (by...

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Autor principal:	Oliveira, Viviana Maria Araújo de
Outros Autores:	Carneiro, Ana Lúcia Basílio, Caúper, Gláucia Socorro de Barros, Pohlit, Adrian Martin
Grau:	Artigo
Idioma:	English
Publicado em:	Acta Amazonica 2020
Assuntos:	Adolpho Ducke Reserve Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda Reserva Florestal Adolpho Ducke Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/13049

id	oai:repositorio:1-13049
recordtype	dspace
spelling	oai:repositorio:1-13049 In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood Testes in vitro de plantas Amazônicas para atividade hemolítica e inibição da agregação plaquetária em sangue humano Oliveira, Viviana Maria Araújo de Carneiro, Ana Lúcia Basílio Caúper, Gláucia Socorro de Barros Pohlit, Adrian Martin Adolpho Ducke Reserve Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda Reserva Florestal Adolpho Ducke Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda In the present study, different aerial parts from twelve Amazonian plant species found in the National Institute for Amazon Research's (INPA's) Adolpho Ducke Forest Reserve (in Manaus, Amazonas, Brazil) were collected. Separate portions of dried, ground plant materials were extracted with water (by infusion), methanol and chloroform (by continuous liquid-solid extraction) and solvents were removed first by rotary evaporation, and finally by freeze-drying which yielded a total of seventy-one freeze-dried extracts for evaluation. These extracts were evaluated initially at concentrations of 500 and 100 µg/mL for in vitro hemolytic activity and in vitro inhibition of platelet aggregation in human blood, respectively. Sixteen extracts (23 % of all extracts tested, 42 % of all plant species), representing the following plantsChaunochiton kappleri (Olacaceae), Diclinanona calycina (Annonaceae), Paypayrola grandiflora (Violaceae), Pleurisanthes parviflora (Icacinaceae), Sarcaulus brasiliensis (Sapotaceae), exhibited significant inhibitory activity towards human platelet aggregation. A group of extracts with antiplatelet aggregation activity having no in vitro hemolytic activity has therefore been identified. Three extracts (4 %), all derived from Elaeoluma nuda (Sapotaceae), exhibited hemolytic activity. None of the plant species in this study has known use in traditional medicine. So, these data serve as a baseline or minimum of antiplatelet and hemolytic activities (and potential usefulness) of non-medicinal plants from the Amazon forest. Finally, in general, these are the first data on hemolytic and inhibitory activity on platelet aggregation for the genera which these plant species represent. No presente estudo, partes aéreas obtidas de doze (12) espécies vegetais da Amazônia encontradas na Reserva Florestal Adolpho Ducke (localizada na cidade de Manaus, Estado do Amazonas, Brasil) do Instituto Nacional de Pesquisas da Amazônia foram coletadas, secadas e moídas. Porções dos materiais vegetais em pó foram extraídas com água (por infusão), metanol e clorofórmio (por extração líquido-sólido contínua) e os solventes foram removidos por evaporação rotatória e finalmente liofilização, forneceram um total de setenta e one (71) extratos liofilizados. Esses extratos foram avaliados inicialmente para atividade hemolítica in vitro e atividade inibitória da agregação plaquetária em sangue humano in vitro em concentrações de 500 e 100 µg/mL, respectivamente. Dezesseis (16) extratos (23 % dos extratos testados, 42 % das espécies vegetais) representando as seguintes plantas, apresentaram inibição significativa frente a agregação de plaquetas humanas in vitro: Chaunochiton kappleri (Olacaceae), Diclinanona calycina (Annonaceae), Paypayrola grandiflora (Violaceae), Pleurisanthes parviflora (Icacinaceae), Sarcaulus brasiliensis (Sapotaceae). Como principal resultado, um grupo de extratos apresentando atividade inibitória da agregação plaquetária e em que não há atividade hemolítica in vitro foi identificado. Três (3) extratos (4 % do total de extratos testados), todos obtidos a partir de Elaeoluma nuda (Sapotaceae), apresentaram atividade hemolítica. Nenhuma das espécies vegetais nesse estudo tem uso medicinal conhecido. Assim, esses dados servem de linha base ou mínimas das atividades antiplaquetária e hemolítica (e utilidade potencial) de plantas da floresta Amazônica. Finalmente, em geral, esses dados são os primeiros disponíveis sobre ação hemolítica e inibição da agregação plaquetária dos gêneros representados por essas espécies de plantas. 2020-04-24T15:13:50Z 2020-04-24T15:13:50Z 2009 Artigo https://repositorio.inpa.gov.br/handle/1/13049 10.1590/S0044-59672009000400026 en Volume 39, Número 4, Pags. 973-980 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Acta Amazonica
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Adolpho Ducke Reserve Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda Reserva Florestal Adolpho Ducke Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda
spellingShingle	Adolpho Ducke Reserve Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda Reserva Florestal Adolpho Ducke Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda Oliveira, Viviana Maria Araújo de In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood
topic_facet	Adolpho Ducke Reserve Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda Reserva Florestal Adolpho Ducke Chaunochiton Kappleri Diclinanona Calycina Elaeoluma Nuda
description	In the present study, different aerial parts from twelve Amazonian plant species found in the National Institute for Amazon Research's (INPA's) Adolpho Ducke Forest Reserve (in Manaus, Amazonas, Brazil) were collected. Separate portions of dried, ground plant materials were extracted with water (by infusion), methanol and chloroform (by continuous liquid-solid extraction) and solvents were removed first by rotary evaporation, and finally by freeze-drying which yielded a total of seventy-one freeze-dried extracts for evaluation. These extracts were evaluated initially at concentrations of 500 and 100 µg/mL for in vitro hemolytic activity and in vitro inhibition of platelet aggregation in human blood, respectively. Sixteen extracts (23 % of all extracts tested, 42 % of all plant species), representing the following plantsChaunochiton kappleri (Olacaceae), Diclinanona calycina (Annonaceae), Paypayrola grandiflora (Violaceae), Pleurisanthes parviflora (Icacinaceae), Sarcaulus brasiliensis (Sapotaceae), exhibited significant inhibitory activity towards human platelet aggregation. A group of extracts with antiplatelet aggregation activity having no in vitro hemolytic activity has therefore been identified. Three extracts (4 %), all derived from Elaeoluma nuda (Sapotaceae), exhibited hemolytic activity. None of the plant species in this study has known use in traditional medicine. So, these data serve as a baseline or minimum of antiplatelet and hemolytic activities (and potential usefulness) of non-medicinal plants from the Amazon forest. Finally, in general, these are the first data on hemolytic and inhibitory activity on platelet aggregation for the genera which these plant species represent.
format	Artigo
author	Oliveira, Viviana Maria Araújo de
author2	Carneiro, Ana Lúcia Basílio Caúper, Gláucia Socorro de Barros Pohlit, Adrian Martin
author2Str	Carneiro, Ana Lúcia Basílio Caúper, Gláucia Socorro de Barros Pohlit, Adrian Martin
title	In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood
title_short	In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood
title_full	In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood
title_fullStr	In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood
title_full_unstemmed	In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood
title_sort	in vitro screening of amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood
publisher	Acta Amazonica
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/13049
_version_	1787141746708185088
score	11.755432

In vitro screening of Amazonian plants for hemolytic activity and inhibition of platelet aggregation in human blood

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