Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus

Oliveira, Sarah Ragonha de; Souza, Rondon Tatsuta Yamane Baptista de; Brasil, Elenice Martins; Andrade, Jaqueline Inês Alves de; Nunes, Érica da Silva Santiago; Ono, Eduardo Akifumi; Affonso, Elizabeth Gusmão

Artigo

Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus

In order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutionsT1 - Control (0.9% NaCl saline solution); T2 (4 x 10(...

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Autor principal:	Oliveira, Sarah Ragonha de
Outros Autores:	Souza, Rondon Tatsuta Yamane Baptista de, Brasil, Elenice Martins, Andrade, Jaqueline Inês Alves de, Nunes, Érica da Silva Santiago, Ono, Eduardo Akifumi, Affonso, Elizabeth Gusmão
Grau:	Artigo
Idioma:	English
Publicado em:	Acta Amazonica 2020
Assuntos:	Aeromonas Hydrophila 96-h Ld50 Matrinxã Tropical Fishes Aeromonas Hydrophila Dl50 96-h Matrinxã Peixes Tropicais
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/13050

id	oai:repositorio:1-13050
recordtype	dspace
spelling	oai:repositorio:1-13050 Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus DL5o da bactéria Aeromonas hydrophila para o matrinxã, Brycon amazonicus Oliveira, Sarah Ragonha de Souza, Rondon Tatsuta Yamane Baptista de Brasil, Elenice Martins Andrade, Jaqueline Inês Alves de Nunes, Érica da Silva Santiago Ono, Eduardo Akifumi Affonso, Elizabeth Gusmão Aeromonas Hydrophila 96-h Ld50 Matrinxã Tropical Fishes Aeromonas Hydrophila Dl50 96-h Matrinxã Peixes Tropicais In order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutionsT1 - Control (0.9% NaCl saline solution); T2 (4 x 10(11) cells/ mL); T3 (5 x 10(11) cells/ mL); T4 (1.36 x 10(12) cells/ mL) and T5 (3.06 x 10(12) cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L-1), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T1 = 0%; T2 = 16.66%; T3 = 44.44%; T4 = 72.22% and T5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD50 value of A. hydrophila to matrinxã is 6.66 x 10(11) cells/ mL. Para determinar a dose letal (DL50 96-h) da bactéria Aeromonas hydrophila para o matrinxã, Brycon amazonicus, com aplicabilidade para testes de desafio, foram utilizados 90 peixes (63,23 ± 6,39 g), divididos em cinco tratamentos, com diferentes soluções bacterianas: T1 - Controle (solução salina 0,9% NaCl); T2 (4 x 10(11) células/ mL); T3 (5 x 10(11) células/ mL-1); T4 (1,36 x 10(12) células/mL-1) e T5 (3,06 x 10(12) células/ mL-1). Os peixes foram previamente anestesiados com benzocaína (60 mg L-1), inoculados na cavidade peritoneal com as suspensões bacterianas e distribuídos em 15 aquários de vidro de 80 L de capacidade, com aeração constante. O experimento teve duração de 96 h, no qual foram monitoradas a mortalidade e a qualidade da água. O delineamento experimental foi inteiramente casualisado com três réplicas e a DL50 96-h foi estimada de acordo com o método Spearman-Karber. Durante o experimento os parâmetros físico-químicos da água permaneceram dentro das condições consideradas adequadas para o desenvolvimento e saúde dos organismos aquáticos. A mortalidade dos peixes aumentou nas concentrações crescentes de A. hydrophila (T1 = 0%; T2 = 16,66%; T3 = 44,44%; T4 = 72,22% e T5 = 100%), contudo, as primeiras mortalidades ocorreram em 57 h após a inoculação das concentrações bacterianas, sendo observados os primeiros sinais de infecção em 24 h após a inoculação. Os resultados indicam que o valor da DL50 96-h da bactéria A. hydrophila para o matrinxã foi igual a 6,66 x 10(11) células/mL de solução salina. 2020-04-24T15:13:51Z 2020-04-24T15:13:51Z 2011 Artigo https://repositorio.inpa.gov.br/handle/1/13050 10.1590/S0044-59672011000200019 en Volume 41, Número 2, Pags. 321-326 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Acta Amazonica
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Aeromonas Hydrophila 96-h Ld50 Matrinxã Tropical Fishes Aeromonas Hydrophila Dl50 96-h Matrinxã Peixes Tropicais
spellingShingle	Aeromonas Hydrophila 96-h Ld50 Matrinxã Tropical Fishes Aeromonas Hydrophila Dl50 96-h Matrinxã Peixes Tropicais Oliveira, Sarah Ragonha de Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus
topic_facet	Aeromonas Hydrophila 96-h Ld50 Matrinxã Tropical Fishes Aeromonas Hydrophila Dl50 96-h Matrinxã Peixes Tropicais
description	In order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutionsT1 - Control (0.9% NaCl saline solution); T2 (4 x 10(11) cells/ mL); T3 (5 x 10(11) cells/ mL); T4 (1.36 x 10(12) cells/ mL) and T5 (3.06 x 10(12) cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L-1), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T1 = 0%; T2 = 16.66%; T3 = 44.44%; T4 = 72.22% and T5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD50 value of A. hydrophila to matrinxã is 6.66 x 10(11) cells/ mL.
format	Artigo
author	Oliveira, Sarah Ragonha de
author2	Souza, Rondon Tatsuta Yamane Baptista de Brasil, Elenice Martins Andrade, Jaqueline Inês Alves de Nunes, Érica da Silva Santiago Ono, Eduardo Akifumi Affonso, Elizabeth Gusmão
author2Str	Souza, Rondon Tatsuta Yamane Baptista de Brasil, Elenice Martins Andrade, Jaqueline Inês Alves de Nunes, Érica da Silva Santiago Ono, Eduardo Akifumi Affonso, Elizabeth Gusmão
title	Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus
title_short	Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus
title_full	Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus
title_fullStr	Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus
title_full_unstemmed	Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus
title_sort	ld5o of the bacteria aeromonas hydrophila to matrinxã, brycon amazonicus
publisher	Acta Amazonica
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/13050
_version_	1787141287243153408
score	11.675608

Ld5O Of The Bacteria Aeromonas Hydrophila To Matrinxã, Brycon Amazonicus

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