Detecção de inibidores de tripsina e atividade hemaglutinante em sementes de leguminosas arbóreas da amazônia
Different classes of proteins are common in Leguminosae seeds, including trypsin inhibitors and hemagglutinin proteins, which act on proteolytic enzymes and cell-surface carbohydrates, respectively. The aim of this work was to quantify, to detect and characterize partially these proteins in seeds of...
| Autor principal: | Chevreuil, Larissa Ramos |
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| Outros Autores: | Gonçalves, José Francisco Carvalho de, Bariani, Adriana, Rordrigues, João Victor Figueiredo Cardoso, Pinto, Ana Cristina da Silva |
| Grau: | Artigo |
| Idioma: | pt_BR |
| Publicado em: |
Acta Amazonica
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/13568 |
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oai:repositorio:1-13568 |
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oai:repositorio:1-13568 Detecção de inibidores de tripsina e atividade hemaglutinante em sementes de leguminosas arbóreas da amazônia Detection of trypsin inhibitors and hemagglutinating activity in tree leguminous seeds of amazonian Chevreuil, Larissa Ramos Gonçalves, José Francisco Carvalho de Bariani, Adriana Rordrigues, João Victor Figueiredo Cardoso Pinto, Ana Cristina da Silva Serine Proteinases Seeds Lectins Erythrocytes Serinoproteinases Sementes Lectinas Eritrócitos Different classes of proteins are common in Leguminosae seeds, including trypsin inhibitors and hemagglutinin proteins, which act on proteolytic enzymes and cell-surface carbohydrates, respectively. The aim of this work was to quantify, to detect and characterize partially these proteins in seeds of Tachigali plumbea, Sesbania exasperata and Ormosia costulata var. trifoliata. Seeds of the three species were powdered and submitted to an extraction with a saline solution (NaCl 0.15M - 10%, p/v). The resulting total extracts were used to quantify proteins content, detect the residual trypsin activity, hemagglutinating activity (AHE) and the proteic profile. Residual trypsin activity was observed only for T. plumbea and S. exasperata, which values were 4 and 19% respectively. AHE was detected in extracts of all three species, total extracts of T. plumbea and S. exasperata hemagglutinated erythrocytes of rats, mice and hamsters, whereas O. costulata had this effect only on hamster erythrocytes. The proteic profile obtained by SDS-PAGE showed that T. plumbea and S. exasperata have a higher content of protein with an apparent molecular mass of 10 - 30 kDa, while O. costulata predominantly contains proteic bands with molecular masses varying between 20 to 25 kDa. It is concluded that total extracts of O. costulata and S. exasperata, species of the subfamily Papilionoideae, present less trypsin inhibitors than T. plumbea (Caesalpinioideae). AHE, both in form of minimum hemagglutinin concentration and the specified interaction with erythrocytes, differed even among species from the same subfamily. Diferentes classes de proteínas são comuns em sementes de leguminosas, incluindo inibidores de tripsina e proteínas hemaglutinantes, as quais atuam sobre enzimas proteolíticas e sobre carboidratos da superfície celular, respectivamente. O objetivo deste trabalho foi quantificar, detectar e caracterizar parcialmente a ocorrência dessas proteínas em sementes de Tachigali plumbea, Sesbania exasperata e Ormosia costulata var. trifoliolata. Sementes das três espécies foram moídas e submetidas à extração salina (NaCl 0,15M - 10 %, p/v). Os extratos totais obtidos foram utilizados para quantificar o conteúdo protéico, detectar a atividade residual da tripsina, a atividade hemaglutinante (AHE) e na obtenção do perfil protéico. A atividade residual da tripsina foi observada somente para T. plumbea e S. exasperata, cujos valores foram 4 e 19 %, respectivamente. A AHE foi detectada nos extratos das três espécies, sendo que os extratos totais de T. plumbea e S. exasperata, hemaglutinaram eritrócitos de rato, camundongo e hamster, enquanto que a espécie O. costulata hemaglutinou somente eritrócitos de hamster. O perfil protéico em SDS-PAGE revelou maior ocorrência de proteínas com massa molecular aparente de 10 a 30 kDa para T. plumbea e S. exasperata, enquanto que para O. costulata prevaleceram bandas protéicas com massa molecular variando entre 20-25 kDa. Conclui-se que os extratos totais de O. costulata e S. exasperata, pertencentes à subfamília Papilionoideae, apresentam menor conteúdo de inibidores de tripsina que T. plumbea (Caesalpinioideae) e, quanto à AHE, os resultados mostraram-se diferenciados, mesmo entre as espécies da mesma subfamília, tanto para a concentração mínima hemaglutinante quanto para a especificidade de interação com os eritrócitos. 2020-04-24T15:31:07Z 2020-04-24T15:31:07Z 2009 Artigo https://repositorio.inpa.gov.br/handle/1/13568 10.1590/S0044-59672009000100021 pt_BR Volume 39, Número 1, Pags. 199-205 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Acta Amazonica |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
pt_BR |
| topic |
Serine Proteinases Seeds Lectins Erythrocytes Serinoproteinases Sementes Lectinas Eritrócitos |
| spellingShingle |
Serine Proteinases Seeds Lectins Erythrocytes Serinoproteinases Sementes Lectinas Eritrócitos Chevreuil, Larissa Ramos Detecção de inibidores de tripsina e atividade hemaglutinante em sementes de leguminosas arbóreas da amazônia |
| topic_facet |
Serine Proteinases Seeds Lectins Erythrocytes Serinoproteinases Sementes Lectinas Eritrócitos |
| description |
Different classes of proteins are common in Leguminosae seeds, including trypsin inhibitors and hemagglutinin proteins, which act on proteolytic enzymes and cell-surface carbohydrates, respectively. The aim of this work was to quantify, to detect and characterize partially these proteins in seeds of Tachigali plumbea, Sesbania exasperata and Ormosia costulata var. trifoliata. Seeds of the three species were powdered and submitted to an extraction with a saline solution (NaCl 0.15M - 10%, p/v). The resulting total extracts were used to quantify proteins content, detect the residual trypsin activity, hemagglutinating activity (AHE) and the proteic profile. Residual trypsin activity was observed only for T. plumbea and S. exasperata, which values were 4 and 19% respectively. AHE was detected in extracts of all three species, total extracts of T. plumbea and S. exasperata hemagglutinated erythrocytes of rats, mice and hamsters, whereas O. costulata had this effect only on hamster erythrocytes. The proteic profile obtained by SDS-PAGE showed that T. plumbea and S. exasperata have a higher content of protein with an apparent molecular mass of 10 - 30 kDa, while O. costulata predominantly contains proteic bands with molecular masses varying between 20 to 25 kDa. It is concluded that total extracts of O. costulata and S. exasperata, species of the subfamily Papilionoideae, present less trypsin inhibitors than T. plumbea (Caesalpinioideae). AHE, both in form of minimum hemagglutinin concentration and the specified interaction with erythrocytes, differed even among species from the same subfamily. |
| format |
Artigo |
| author |
Chevreuil, Larissa Ramos |
| author2 |
Gonçalves, José Francisco Carvalho de Bariani, Adriana Rordrigues, João Victor Figueiredo Cardoso Pinto, Ana Cristina da Silva |
| author2Str |
Gonçalves, José Francisco Carvalho de Bariani, Adriana Rordrigues, João Victor Figueiredo Cardoso Pinto, Ana Cristina da Silva |
| title |
Detecção de inibidores de tripsina e atividade hemaglutinante em sementes de leguminosas arbóreas da amazônia |
| title_short |
Detecção de inibidores de tripsina e atividade hemaglutinante em sementes de leguminosas arbóreas da amazônia |
| title_full |
Detecção de inibidores de tripsina e atividade hemaglutinante em sementes de leguminosas arbóreas da amazônia |
| title_fullStr |
Detecção de inibidores de tripsina e atividade hemaglutinante em sementes de leguminosas arbóreas da amazônia |
| title_full_unstemmed |
Detecção de inibidores de tripsina e atividade hemaglutinante em sementes de leguminosas arbóreas da amazônia |
| title_sort |
detecção de inibidores de tripsina e atividade hemaglutinante em sementes de leguminosas arbóreas da amazônia |
| publisher |
Acta Amazonica |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/13568 |
| _version_ |
1787142244713627648 |
| score |
11.675608 |