Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)

Barbosa, André de Figueiredo; Oliveira, Sandra Maria P.; Bertho, Álvaro Luiz; Franco, A. M.; Rangel, Elizabeth Ferreira

Artigo

Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)

Lutzomyia longipalpis females received single and mixed infections with Endotrypanum and Leishmania. Two biological parameters were analyzed: the percentage of infected females and the distribution of flagellates in the gut of the females. The principal comparisons were performed between (1) two str...

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Autor principal:	Barbosa, André de Figueiredo
Outros Autores:	Oliveira, Sandra Maria P., Bertho, Álvaro Luiz, Franco, A. M., Rangel, Elizabeth Ferreira
Grau:	Artigo
Idioma:	English
Publicado em:	Memórias do Instituto Oswaldo Cruz 2020
Assuntos:	Diptera Endotrypanum Kinetoplastida Leishmania Guyanensis Lutzomyia Longipalpis Mastigophora (flagellates) Phlebotominae Psychodidae Trypanosoma Trypanosomatidae Viannia
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/14528

id	oai:repositorio:1-14528
recordtype	dspace
spelling	oai:repositorio:1-14528 Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae) Barbosa, André de Figueiredo Oliveira, Sandra Maria P. Bertho, Álvaro Luiz Franco, A. M. Rangel, Elizabeth Ferreira Diptera Endotrypanum Kinetoplastida Leishmania Guyanensis Lutzomyia Longipalpis Mastigophora (flagellates) Phlebotominae Psychodidae Trypanosoma Trypanosomatidae Viannia Lutzomyia longipalpis females received single and mixed infections with Endotrypanum and Leishmania. Two biological parameters were analyzed: the percentage of infected females and the distribution of flagellates in the gut of the females. The principal comparisons were performed between (1) two strains of Endotrypanum, (2) cloned versus primary sample of one strain of Endotrypanum, (3) Endotrypanum versus Leishmania guyanensis, and (4) the pattern of flagellates behaviour by optical microscopy in females with single or mixed infection versus the identification of parasites isolated from digestive tracts by isoenzyme electrophoresis. Flagellates of Endotrypanum showed distinct patterns of infection suggesting that there is variation between and within strains. The distribution of Endotrypanum and L. guyanensis differed significantly in relation to the colonization of the stomodeal valve. In coinfection with L. guyanensis, a large number of flagellates were seen to be plentifully infecting the stomodeal valve in significantly more specimens than in females infected by Endotrypanum only. However, the electrophoretic profiles of isoenzymes of parasites recovered from all co-infected specimens corresponded to Endotrypanum. This suggests that the mere correlation sand fly infection-biochemical analysis of isolates may induce parasitological incorrect consideration. 2020-04-24T16:49:28Z 2020-04-24T16:49:28Z 2006 Artigo https://repositorio.inpa.gov.br/handle/1/14528 10.1590/S0074-02762006000800005 en Volume 101, Número 8, Pags. 851-856 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Memórias do Instituto Oswaldo Cruz
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Diptera Endotrypanum Kinetoplastida Leishmania Guyanensis Lutzomyia Longipalpis Mastigophora (flagellates) Phlebotominae Psychodidae Trypanosoma Trypanosomatidae Viannia
spellingShingle	Diptera Endotrypanum Kinetoplastida Leishmania Guyanensis Lutzomyia Longipalpis Mastigophora (flagellates) Phlebotominae Psychodidae Trypanosoma Trypanosomatidae Viannia Barbosa, André de Figueiredo Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)
topic_facet	Diptera Endotrypanum Kinetoplastida Leishmania Guyanensis Lutzomyia Longipalpis Mastigophora (flagellates) Phlebotominae Psychodidae Trypanosoma Trypanosomatidae Viannia
description	Lutzomyia longipalpis females received single and mixed infections with Endotrypanum and Leishmania. Two biological parameters were analyzed: the percentage of infected females and the distribution of flagellates in the gut of the females. The principal comparisons were performed between (1) two strains of Endotrypanum, (2) cloned versus primary sample of one strain of Endotrypanum, (3) Endotrypanum versus Leishmania guyanensis, and (4) the pattern of flagellates behaviour by optical microscopy in females with single or mixed infection versus the identification of parasites isolated from digestive tracts by isoenzyme electrophoresis. Flagellates of Endotrypanum showed distinct patterns of infection suggesting that there is variation between and within strains. The distribution of Endotrypanum and L. guyanensis differed significantly in relation to the colonization of the stomodeal valve. In coinfection with L. guyanensis, a large number of flagellates were seen to be plentifully infecting the stomodeal valve in significantly more specimens than in females infected by Endotrypanum only. However, the electrophoretic profiles of isoenzymes of parasites recovered from all co-infected specimens corresponded to Endotrypanum. This suggests that the mere correlation sand fly infection-biochemical analysis of isolates may induce parasitological incorrect consideration.
format	Artigo
author	Barbosa, André de Figueiredo
author2	Oliveira, Sandra Maria P. Bertho, Álvaro Luiz Franco, A. M. Rangel, Elizabeth Ferreira
author2Str	Oliveira, Sandra Maria P. Bertho, Álvaro Luiz Franco, A. M. Rangel, Elizabeth Ferreira
title	Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)
title_short	Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)
title_full	Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)
title_fullStr	Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)
title_full_unstemmed	Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)
title_sort	single and concomitant experimental infections by endotrypanum spp. and leishmania (viannia) guyanensis (kinetoplastida: trypanosomatidae) in the neotropical sand fly lutzomyia longipalpis (diptera: psychodidae)
publisher	Memórias do Instituto Oswaldo Cruz
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/14528
_version_	1787142744364285952
score	11.755432

Single and concomitant experimental infections by Endotrypanum spp. and Leishmania (Viannia) guyanensis (Kinetoplastida: Trypanosomatidae) in the neotropical sand fly Lutzomyia longipalpis (Diptera: Psychodidae)

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