Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil
An isoenzymatic comparative analysis of the variability and genetics differentiation among Anopheles species was done in populations of An. (Nys.) intermedius and An. (Ano.) mattogrossensis of the Anopheles subgenus, and of An. darlingi, An. albitarsis and An. triannulatus of the Nyssorhynchus subge...
| Autor principal: | Santos, Joselita Maria Mendes dos |
|---|---|
| Outros Autores: | Maia, Juracy de Freitas, Tadei, Wanderli Pedro, Rodriguez, Gloria Alicia Diaz |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Memórias do Instituto Oswaldo Cruz
2020
|
| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14534 |
| id |
oai:repositorio:1-14534 |
|---|---|
| recordtype |
dspace |
| spelling |
oai:repositorio:1-14534 Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil Santos, Joselita Maria Mendes dos Maia, Juracy de Freitas Tadei, Wanderli Pedro Rodriguez, Gloria Alicia Diaz Isoenzyme Allele Animals Experiment Anopheles Brasil Controlled Study Genetic Distance Genetic Variability Heterozygosity Morphology Nonhuman Phylogeny Polymorphic Locus Animals Classification Disease Carrier Enzymology Gene Genetic Variability Genetics Species Difference Albitarsis Animalsia Nyssorhynchus Alleles Animal Anopheles Brasil Genes, Insect Insect Vectors Isoenzymes Species Specificity Variation (genetics) An isoenzymatic comparative analysis of the variability and genetics differentiation among Anopheles species was done in populations of An. (Nys.) intermedius and An. (Ano.) mattogrossensis of the Anopheles subgenus, and of An. darlingi, An. albitarsis and An. triannulatus of the Nyssorhynchus subgenus, with the aim of detecting differences between both subgenera and of estimating the degree of genetic interespecific divergence. Samples from Macapá, State of Amapá and Janauari Lake, near Manaus, State of Amazonas, were analyzed for eight isoenzymatic loci. Analysis revealed differences in the average number of alleles per locus (1.6-2.3) and heterozygosity (0.060-0.284). However, the proportion of polymorphic loci was the same for An. (Nys.) darlingi, An. (Nys.) triannulatus and An. (Ano.) mattogrossensis (50%), but differed for An. (Nys.) albitarsis (62.5%) and An. (Ano.) intermedius (25%). Only the IDH1 (P > 0.5) locus in all species studied was in Hardy-Weinberg equilibrium. The fixation index demonstrated elevated genetic structuring among species, based on values of Fst = 0.644 and genetic distance (0.344-0.989). Genetic difference was higher between An. (Nys.) triannulatus and An. (Ano.) intermedius (0.989) and smaller between An. (Nys.) albitarsis sensu lato and An. (Nys.) darlingi (0.344). The data show interspecific genetic divergence which differs from the phylogenetic hypothesis based on morphological characters. 2020-04-24T16:49:32Z 2020-04-24T16:49:32Z 2003 Artigo https://repositorio.inpa.gov.br/handle/1/14534 10.1590/S0074-02762003000200014 en Volume 98, Número 2, Pags. 247-253 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Memórias do Instituto Oswaldo Cruz |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Isoenzyme Allele Animals Experiment Anopheles Brasil Controlled Study Genetic Distance Genetic Variability Heterozygosity Morphology Nonhuman Phylogeny Polymorphic Locus Animals Classification Disease Carrier Enzymology Gene Genetic Variability Genetics Species Difference Albitarsis Animalsia Nyssorhynchus Alleles Animal Anopheles Brasil Genes, Insect Insect Vectors Isoenzymes Species Specificity Variation (genetics) |
| spellingShingle |
Isoenzyme Allele Animals Experiment Anopheles Brasil Controlled Study Genetic Distance Genetic Variability Heterozygosity Morphology Nonhuman Phylogeny Polymorphic Locus Animals Classification Disease Carrier Enzymology Gene Genetic Variability Genetics Species Difference Albitarsis Animalsia Nyssorhynchus Alleles Animal Anopheles Brasil Genes, Insect Insect Vectors Isoenzymes Species Specificity Variation (genetics) Santos, Joselita Maria Mendes dos Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil |
| topic_facet |
Isoenzyme Allele Animals Experiment Anopheles Brasil Controlled Study Genetic Distance Genetic Variability Heterozygosity Morphology Nonhuman Phylogeny Polymorphic Locus Animals Classification Disease Carrier Enzymology Gene Genetic Variability Genetics Species Difference Albitarsis Animalsia Nyssorhynchus Alleles Animal Anopheles Brasil Genes, Insect Insect Vectors Isoenzymes Species Specificity Variation (genetics) |
| description |
An isoenzymatic comparative analysis of the variability and genetics differentiation among Anopheles species was done in populations of An. (Nys.) intermedius and An. (Ano.) mattogrossensis of the Anopheles subgenus, and of An. darlingi, An. albitarsis and An. triannulatus of the Nyssorhynchus subgenus, with the aim of detecting differences between both subgenera and of estimating the degree of genetic interespecific divergence. Samples from Macapá, State of Amapá and Janauari Lake, near Manaus, State of Amazonas, were analyzed for eight isoenzymatic loci. Analysis revealed differences in the average number of alleles per locus (1.6-2.3) and heterozygosity (0.060-0.284). However, the proportion of polymorphic loci was the same for An. (Nys.) darlingi, An. (Nys.) triannulatus and An. (Ano.) mattogrossensis (50%), but differed for An. (Nys.) albitarsis (62.5%) and An. (Ano.) intermedius (25%). Only the IDH1 (P > 0.5) locus in all species studied was in Hardy-Weinberg equilibrium. The fixation index demonstrated elevated genetic structuring among species, based on values of Fst = 0.644 and genetic distance (0.344-0.989). Genetic difference was higher between An. (Nys.) triannulatus and An. (Ano.) intermedius (0.989) and smaller between An. (Nys.) albitarsis sensu lato and An. (Nys.) darlingi (0.344). The data show interspecific genetic divergence which differs from the phylogenetic hypothesis based on morphological characters. |
| format |
Artigo |
| author |
Santos, Joselita Maria Mendes dos |
| author2 |
Maia, Juracy de Freitas Tadei, Wanderli Pedro Rodriguez, Gloria Alicia Diaz |
| author2Str |
Maia, Juracy de Freitas Tadei, Wanderli Pedro Rodriguez, Gloria Alicia Diaz |
| title |
Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil |
| title_short |
Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil |
| title_full |
Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil |
| title_fullStr |
Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil |
| title_full_unstemmed |
Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil |
| title_sort |
isoenzymatic variability among five anopheles species belonging to the nyssorhynchus and anopheles subgenera of the amazon region, brazil |
| publisher |
Memórias do Instituto Oswaldo Cruz |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14534 |
| _version_ |
1787143709782966272 |
| score |
11.755432 |