In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae)

Silva, Luiz Francisco Rocha e; Lima, Emerson Silva; Vasconcellos, Marne Carvalho de; Aranha, Ellen Suzany Pereira; Costa, David Siqueira; Mustafa, Elba Vieira; Morais, Sabrina Kelly Reis de; Alecrim, Maria das Graças Costa; Nunomura, Sergio Massayoshi; Struwe, Lena; Andrade Neto, Valter Ferreira de; Pohlit, Adrian Martin

Artigo

In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae)

Tachia sp. are used as antimalarials in the Amazon Region and in vivo antimalarial activity of a Tachia sp. has been previously reported. Tachia grandiflora Maguire and Weaver is an Amazonian antimalarial plant and herein its cytotoxicity and antimalarial activity were investigated. Spectral analysi...

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Autor principal:	Silva, Luiz Francisco Rocha e
Outros Autores:	Lima, Emerson Silva, Vasconcellos, Marne Carvalho de, Aranha, Ellen Suzany Pereira, Costa, David Siqueira, Mustafa, Elba Vieira, Morais, Sabrina Kelly Reis de, Alecrim, Maria das Graças Costa, Nunomura, Sergio Massayoshi, Struwe, Lena, Andrade Neto, Valter Ferreira de, Pohlit, Adrian Martin
Grau:	Artigo
Idioma:	English
Publicado em:	Memórias do Instituto Oswaldo Cruz 2020
Assuntos:	Antimalarial Agent Plant Extract Animals Chemistry Drug Effect Fibroblast Gentianaceae Human Ic 50 Isolation And Purification Mouse Plasmodium Falciparum Animal Antimalarials Fibroblasts Gentianaceae Humans Inhibitory Concentration 50 Mice Plant Extracts Plasmodium Falciparum Gentianaceae Mus Plasmodium Berghei Plasmodium Falciparum Tachia Tachia Grandiflora
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/14571

id	oai:repositorio:1-14571
recordtype	dspace
spelling	oai:repositorio:1-14571 In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae) Silva, Luiz Francisco Rocha e Lima, Emerson Silva Vasconcellos, Marne Carvalho de Aranha, Ellen Suzany Pereira Costa, David Siqueira Mustafa, Elba Vieira Morais, Sabrina Kelly Reis de Alecrim, Maria das Graças Costa Nunomura, Sergio Massayoshi Struwe, Lena Andrade Neto, Valter Ferreira de Pohlit, Adrian Martin Antimalarial Agent Plant Extract Animals Chemistry Drug Effect Fibroblast Gentianaceae Human Ic 50 Isolation And Purification Mouse Plasmodium Falciparum Animal Antimalarials Fibroblasts Gentianaceae Humans Inhibitory Concentration 50 Mice Plant Extracts Plasmodium Falciparum Gentianaceae Mus Plasmodium Berghei Plasmodium Falciparum Tachia Tachia Grandiflora Tachia sp. are used as antimalarials in the Amazon Region and in vivo antimalarial activity of a Tachia sp. has been previously reported. Tachia grandiflora Maguire and Weaver is an Amazonian antimalarial plant and herein its cytotoxicity and antimalarial activity were investigated. Spectral analysis of the tetraoxygenated xanthone decussatin and the iridoid aglyone amplexine isolated, respectively, from the chloroform fractions of root methanol and leaf ethanol extracts was performed. In vitro inhibition of the growth of Plasmodium falciparum Welch was evaluated using optical microscopy on blood smears. Crude extracts of leaves and roots were inactive in vitro. However, chloroform fractions of the root and leaf extracts [half-maximal inhibitory concentration (IC50) = 10.5 and 35.8 μg/mL, respectively] and amplexine (IC50 = 7.1 μg/mL) were active in vitro. Extracts and fractions were not toxic to type MRC-5 human fibroblasts (IC50 > 50 μg/mL). Water extracts of the roots of T. grandiflora administered by mouth were the most active extracts in the Peters 4-day suppression test in Plasmodium berghei-infected mice. At 500 mg/kg/day, these extracts exhibited 45-59% inhibition five to seven days after infection. T. grandiflora infusions, fractions and isolated substance have potential as antimalarials. 2020-04-24T16:49:57Z 2020-04-24T16:49:57Z 2013 Artigo https://repositorio.inpa.gov.br/handle/1/14571 10.1590/0074-0276108042013017 en Volume 108, Número 4, Pags. 501-507 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Memórias do Instituto Oswaldo Cruz
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Antimalarial Agent Plant Extract Animals Chemistry Drug Effect Fibroblast Gentianaceae Human Ic 50 Isolation And Purification Mouse Plasmodium Falciparum Animal Antimalarials Fibroblasts Gentianaceae Humans Inhibitory Concentration 50 Mice Plant Extracts Plasmodium Falciparum Gentianaceae Mus Plasmodium Berghei Plasmodium Falciparum Tachia Tachia Grandiflora
spellingShingle	Antimalarial Agent Plant Extract Animals Chemistry Drug Effect Fibroblast Gentianaceae Human Ic 50 Isolation And Purification Mouse Plasmodium Falciparum Animal Antimalarials Fibroblasts Gentianaceae Humans Inhibitory Concentration 50 Mice Plant Extracts Plasmodium Falciparum Gentianaceae Mus Plasmodium Berghei Plasmodium Falciparum Tachia Tachia Grandiflora Silva, Luiz Francisco Rocha e In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae)
topic_facet	Antimalarial Agent Plant Extract Animals Chemistry Drug Effect Fibroblast Gentianaceae Human Ic 50 Isolation And Purification Mouse Plasmodium Falciparum Animal Antimalarials Fibroblasts Gentianaceae Humans Inhibitory Concentration 50 Mice Plant Extracts Plasmodium Falciparum Gentianaceae Mus Plasmodium Berghei Plasmodium Falciparum Tachia Tachia Grandiflora
description	Tachia sp. are used as antimalarials in the Amazon Region and in vivo antimalarial activity of a Tachia sp. has been previously reported. Tachia grandiflora Maguire and Weaver is an Amazonian antimalarial plant and herein its cytotoxicity and antimalarial activity were investigated. Spectral analysis of the tetraoxygenated xanthone decussatin and the iridoid aglyone amplexine isolated, respectively, from the chloroform fractions of root methanol and leaf ethanol extracts was performed. In vitro inhibition of the growth of Plasmodium falciparum Welch was evaluated using optical microscopy on blood smears. Crude extracts of leaves and roots were inactive in vitro. However, chloroform fractions of the root and leaf extracts [half-maximal inhibitory concentration (IC50) = 10.5 and 35.8 μg/mL, respectively] and amplexine (IC50 = 7.1 μg/mL) were active in vitro. Extracts and fractions were not toxic to type MRC-5 human fibroblasts (IC50 > 50 μg/mL). Water extracts of the roots of T. grandiflora administered by mouth were the most active extracts in the Peters 4-day suppression test in Plasmodium berghei-infected mice. At 500 mg/kg/day, these extracts exhibited 45-59% inhibition five to seven days after infection. T. grandiflora infusions, fractions and isolated substance have potential as antimalarials.
format	Artigo
author	Silva, Luiz Francisco Rocha e
author2	Lima, Emerson Silva Vasconcellos, Marne Carvalho de Aranha, Ellen Suzany Pereira Costa, David Siqueira Mustafa, Elba Vieira Morais, Sabrina Kelly Reis de Alecrim, Maria das Graças Costa Nunomura, Sergio Massayoshi Struwe, Lena Andrade Neto, Valter Ferreira de Pohlit, Adrian Martin
author2Str	Lima, Emerson Silva Vasconcellos, Marne Carvalho de Aranha, Ellen Suzany Pereira Costa, David Siqueira Mustafa, Elba Vieira Morais, Sabrina Kelly Reis de Alecrim, Maria das Graças Costa Nunomura, Sergio Massayoshi Struwe, Lena Andrade Neto, Valter Ferreira de Pohlit, Adrian Martin
title	In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae)
title_short	In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae)
title_full	In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae)
title_fullStr	In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae)
title_full_unstemmed	In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae)
title_sort	in vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (gentianaceae)
publisher	Memórias do Instituto Oswaldo Cruz
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/14571
_version_	1787143324039118848
score	11.755432

In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the amazonian plant tachia grandiflora (Gentianaceae)

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