Single nucleotide polymorphisms of the genes IL-2, IL-2RB, and JAK3 in patients with cutaneous leishmaniasis caused by Leishmania (V.) guyanensis in Manaus, Amazonas, Brazil
Leishmaniasis is a disease caused by intracellular protozoan parasites of the genus Leishmania. In endemic areas, only a portion of exposed subjects develops cutaneous leishmaniasis (CL), suggesting that the genetic inheritance of the host plays a vital role in both resistance and susceptibility to...
| Autor principal: | Araújo Santos, Felipe Jules de |
|---|---|
| Outros Autores: | Silva, Lener Santos da, Santo, José do Espírito, Mesquita, Tirza Gabrielle Ramos de, Souza, Mara Lúcia Gomes de, Andrade, Moacir Couto de, Talhari, Sine?io, Ramasawmy, Rajendranath |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
PLoS ONE
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14651 |
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oai:repositorio:1-14651 |
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oai:repositorio:1-14651 Single nucleotide polymorphisms of the genes IL-2, IL-2RB, and JAK3 in patients with cutaneous leishmaniasis caused by Leishmania (V.) guyanensis in Manaus, Amazonas, Brazil Araújo Santos, Felipe Jules de Silva, Lener Santos da Santo, José do Espírito Mesquita, Tirza Gabrielle Ramos de Souza, Mara Lúcia Gomes de Andrade, Moacir Couto de Talhari, Sine?io Ramasawmy, Rajendranath Interleukin-2 Interleukin 2 Receptor Beta Janus Kinase 3 Il2 Protein, Human Il2rb Protein, Human Interleukin-2 Interleukin 2 Receptor Beta Jak3 Protein, Mouse Janus Kinase 3 Adult Amazonas Controlled Study Endemic Disease Female Gene Expression Gene Frequency Genetic Association Genotype Human Il 2 Gene Il 2rb Gene Intron Jak3 Gene Leishmania Guyanensis Major Clinical Study Male Polymerase Chain Reaction Promoter Region Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Skin Leishmaniasis American Indian Brasil Clinical Trial Genetic Predisposition Genetics Middle Aged Skin Leishmaniasis Adult Brasil Female Gene Frequency Genetic Predisposition To Disease Genotype Humans Indians, South American Interleukin-2 Interleukin-2 Receptor Beta Subunit Introns Janus Kinase 3 Leishmania Guyanensis Leishmaniasis, Mucocutaneous Male Middle Aged Polymorphism, Single Nucleotide Promoter Regions, Genetic Leishmaniasis is a disease caused by intracellular protozoan parasites of the genus Leishmania. In endemic areas, only a portion of exposed subjects develops cutaneous leishmaniasis (CL), suggesting that the genetic inheritance of the host plays a vital role in both resistance and susceptibility to the disease. Interleukin-2 (IL-2) is a cytokine that plays a central role in the regulation of the immune response in infection through the axis IL-2/IL-2R (receptor) complex, triggering a series of intracellular events, among which the signaling of Janus kinase/signal transducers and activators of transcription (JAK-STAT). The present study aimed at verifying the possible relationship between single nucleotide polymorphism (s) (SNP s) in the genes IL-2, IL-2RB, and JAK3 in subjects with CL caused by Leishmania guyanensis in the city of Manaus, state of Amazonas, Brazil. 820 patients with CL and 850 healthy subjects (control group) coming from the same endemic areas as the patients were examined. The SNPs -2425G/A (rs4833248) and -330 T/G (rs2069762), located in the IL-2 gene promoter region, seem to influence the expression of the gene and the SNP +10558G/A (rs1003694) and +13295T/C (rs3212760) located in the 3rd intron of the IL-2RB gene and the 13th intron of the JAK3 gene, respectively, were studied by PCR-RFLP. Genotypes and alleles frequencies were obtained by direct counting. For the comparison between the two groups, the χ2 test with OR (odds ratio) and the 95% confidence interval (CI) were used. Similar genotypes and alleles frequencies for the different SNPs were observed in both patients with CL and healthy controls. Comparison of genotypic and allelic frequency between patients with CL and healthy subjects did not show any difference. These polymorphisms do not predict susceptibility to, or protection against the development of CL caused by L. guyanensis in the Amazonas. © 2019 de Araújo Santos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2020-04-24T16:59:56Z 2020-04-24T16:59:56Z 2019 Artigo https://repositorio.inpa.gov.br/handle/1/14651 10.1371/journal.pone.0220572 en Volume 14, Número 8 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf PLoS ONE |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Interleukin-2 Interleukin 2 Receptor Beta Janus Kinase 3 Il2 Protein, Human Il2rb Protein, Human Interleukin-2 Interleukin 2 Receptor Beta Jak3 Protein, Mouse Janus Kinase 3 Adult Amazonas Controlled Study Endemic Disease Female Gene Expression Gene Frequency Genetic Association Genotype Human Il 2 Gene Il 2rb Gene Intron Jak3 Gene Leishmania Guyanensis Major Clinical Study Male Polymerase Chain Reaction Promoter Region Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Skin Leishmaniasis American Indian Brasil Clinical Trial Genetic Predisposition Genetics Middle Aged Skin Leishmaniasis Adult Brasil Female Gene Frequency Genetic Predisposition To Disease Genotype Humans Indians, South American Interleukin-2 Interleukin-2 Receptor Beta Subunit Introns Janus Kinase 3 Leishmania Guyanensis Leishmaniasis, Mucocutaneous Male Middle Aged Polymorphism, Single Nucleotide Promoter Regions, Genetic |
| spellingShingle |
Interleukin-2 Interleukin 2 Receptor Beta Janus Kinase 3 Il2 Protein, Human Il2rb Protein, Human Interleukin-2 Interleukin 2 Receptor Beta Jak3 Protein, Mouse Janus Kinase 3 Adult Amazonas Controlled Study Endemic Disease Female Gene Expression Gene Frequency Genetic Association Genotype Human Il 2 Gene Il 2rb Gene Intron Jak3 Gene Leishmania Guyanensis Major Clinical Study Male Polymerase Chain Reaction Promoter Region Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Skin Leishmaniasis American Indian Brasil Clinical Trial Genetic Predisposition Genetics Middle Aged Skin Leishmaniasis Adult Brasil Female Gene Frequency Genetic Predisposition To Disease Genotype Humans Indians, South American Interleukin-2 Interleukin-2 Receptor Beta Subunit Introns Janus Kinase 3 Leishmania Guyanensis Leishmaniasis, Mucocutaneous Male Middle Aged Polymorphism, Single Nucleotide Promoter Regions, Genetic Araújo Santos, Felipe Jules de Single nucleotide polymorphisms of the genes IL-2, IL-2RB, and JAK3 in patients with cutaneous leishmaniasis caused by Leishmania (V.) guyanensis in Manaus, Amazonas, Brazil |
| topic_facet |
Interleukin-2 Interleukin 2 Receptor Beta Janus Kinase 3 Il2 Protein, Human Il2rb Protein, Human Interleukin-2 Interleukin 2 Receptor Beta Jak3 Protein, Mouse Janus Kinase 3 Adult Amazonas Controlled Study Endemic Disease Female Gene Expression Gene Frequency Genetic Association Genotype Human Il 2 Gene Il 2rb Gene Intron Jak3 Gene Leishmania Guyanensis Major Clinical Study Male Polymerase Chain Reaction Promoter Region Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Skin Leishmaniasis American Indian Brasil Clinical Trial Genetic Predisposition Genetics Middle Aged Skin Leishmaniasis Adult Brasil Female Gene Frequency Genetic Predisposition To Disease Genotype Humans Indians, South American Interleukin-2 Interleukin-2 Receptor Beta Subunit Introns Janus Kinase 3 Leishmania Guyanensis Leishmaniasis, Mucocutaneous Male Middle Aged Polymorphism, Single Nucleotide Promoter Regions, Genetic |
| description |
Leishmaniasis is a disease caused by intracellular protozoan parasites of the genus Leishmania. In endemic areas, only a portion of exposed subjects develops cutaneous leishmaniasis (CL), suggesting that the genetic inheritance of the host plays a vital role in both resistance and susceptibility to the disease. Interleukin-2 (IL-2) is a cytokine that plays a central role in the regulation of the immune response in infection through the axis IL-2/IL-2R (receptor) complex, triggering a series of intracellular events, among which the signaling of Janus kinase/signal transducers and activators of transcription (JAK-STAT). The present study aimed at verifying the possible relationship between single nucleotide polymorphism (s) (SNP s) in the genes IL-2, IL-2RB, and JAK3 in subjects with CL caused by Leishmania guyanensis in the city of Manaus, state of Amazonas, Brazil. 820 patients with CL and 850 healthy subjects (control group) coming from the same endemic areas as the patients were examined. The SNPs -2425G/A (rs4833248) and -330 T/G (rs2069762), located in the IL-2 gene promoter region, seem to influence the expression of the gene and the SNP +10558G/A (rs1003694) and +13295T/C (rs3212760) located in the 3rd intron of the IL-2RB gene and the 13th intron of the JAK3 gene, respectively, were studied by PCR-RFLP. Genotypes and alleles frequencies were obtained by direct counting. For the comparison between the two groups, the χ2 test with OR (odds ratio) and the 95% confidence interval (CI) were used. Similar genotypes and alleles frequencies for the different SNPs were observed in both patients with CL and healthy controls. Comparison of genotypic and allelic frequency between patients with CL and healthy subjects did not show any difference. These polymorphisms do not predict susceptibility to, or protection against the development of CL caused by L. guyanensis in the Amazonas. © 2019 de Araújo Santos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| format |
Artigo |
| author |
Araújo Santos, Felipe Jules de |
| author2 |
Silva, Lener Santos da Santo, José do Espírito Mesquita, Tirza Gabrielle Ramos de Souza, Mara Lúcia Gomes de Andrade, Moacir Couto de Talhari, Sine?io Ramasawmy, Rajendranath |
| author2Str |
Silva, Lener Santos da Santo, José do Espírito Mesquita, Tirza Gabrielle Ramos de Souza, Mara Lúcia Gomes de Andrade, Moacir Couto de Talhari, Sine?io Ramasawmy, Rajendranath |
| title |
Single nucleotide polymorphisms of the genes IL-2, IL-2RB, and JAK3 in patients with cutaneous leishmaniasis caused by Leishmania (V.) guyanensis in Manaus, Amazonas, Brazil |
| title_short |
Single nucleotide polymorphisms of the genes IL-2, IL-2RB, and JAK3 in patients with cutaneous leishmaniasis caused by Leishmania (V.) guyanensis in Manaus, Amazonas, Brazil |
| title_full |
Single nucleotide polymorphisms of the genes IL-2, IL-2RB, and JAK3 in patients with cutaneous leishmaniasis caused by Leishmania (V.) guyanensis in Manaus, Amazonas, Brazil |
| title_fullStr |
Single nucleotide polymorphisms of the genes IL-2, IL-2RB, and JAK3 in patients with cutaneous leishmaniasis caused by Leishmania (V.) guyanensis in Manaus, Amazonas, Brazil |
| title_full_unstemmed |
Single nucleotide polymorphisms of the genes IL-2, IL-2RB, and JAK3 in patients with cutaneous leishmaniasis caused by Leishmania (V.) guyanensis in Manaus, Amazonas, Brazil |
| title_sort |
single nucleotide polymorphisms of the genes il-2, il-2rb, and jak3 in patients with cutaneous leishmaniasis caused by leishmania (v.) guyanensis in manaus, amazonas, brazil |
| publisher |
PLoS ONE |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14651 |
| _version_ |
1787141301438775296 |
| score |
11.675088 |