Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family
Lebiasinidae is a small fish family composed by miniature to small-sized fishes with few cytogenetic data (most of them limited to descriptions of diploid chromosome numbers), thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing, the f...
| Autor principal: | Toma, Gustavo Akira |
|---|---|
| Outros Autores: | Moraes, Renata Luiza Rosa de, Sassi, Francisco de Menezes Cavalcante, Bertollo, Luiz Antônio Carlos, Oliveira, Ezequiel Aguiar de, Ráb, Petr, Sember, Alexandr, Liehr, Thomas, Hatanaka, Terumi, Viana, Patrik Ferreira, Marinho, Manoela Maria Ferreira, Feldberg, Eliana, Cioffi, Marcelo de Bello |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
PLoS ONE
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14652 |
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oai:repositorio:1-14652 |
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oai:repositorio:1-14652 Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family Toma, Gustavo Akira Moraes, Renata Luiza Rosa de Sassi, Francisco de Menezes Cavalcante Bertollo, Luiz Antônio Carlos Oliveira, Ezequiel Aguiar de Ráb, Petr Sember, Alexandr Liehr, Thomas Hatanaka, Terumi Viana, Patrik Ferreira Marinho, Manoela Maria Ferreira Feldberg, Eliana Cioffi, Marcelo de Bello Dna 18s Dna 5s Repetitive Dna Ribosome Rna Animals Experiment C Banding Centromere Characiformes Chromosome Arm Chromosome Banding Pattern Chromosome Nor Chromosome Rearrangement Comparative Genomic Hybridization Controlled Study Copeina Guttata Cytogenetics Diploidy Dna Determination Female In Situ Hybridization, Fluorescence Giemsa Stain Heterochromatin Karyotype Lebiasina Melanoguttata Lebiasinidae Male Nonhuman Pyrrhulina Brevis Red Spotted Tetra Animals Characiformes Chromosome Classification Genetics Evolution, Molecular Phylogeny Animalss Characiformes Chromosomes Evolution, Molecular Female Karyotype Male Phylogeny Rna, Ribosomal Lebiasinidae is a small fish family composed by miniature to small-sized fishes with few cytogenetic data (most of them limited to descriptions of diploid chromosome numbers), thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing, the first cytogenetic data for the red spotted tetra, Copeina guttata, including the standard karyotype, C-banding, repetitive DNA mapping by fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH), providing chromosomal patterns and novel insights into the karyotype differentiation of the family. Males and females share diploid chromosome number 2n = 42 and karyotype composed of 2 metacentric (m), 4 submetacentric (sm) and 36 subtelocentric to acrocentric (st-a) chromosomes. Blocks of constitutive heterochromatin were observed in the centromeric and interstitial regions of several chromosomes, in addition to a remarkably large distal block, heteromorphic in size, which fully corresponded with the 18S rDNA sites in the fourth chromosomal pair. This overlap was confirmed by 5S/18S rDNA dual-color FISH. On the other hand, 5S rDNA clusters were situated in the long and short arms of the 2nd and 15th pairs, respectively. No sex-linked karyotype differences were revealed by male/female CGH experiments. The genomic probes from other two lebiasinid species, Lebiasina melanoguttata and Pyrrhulina brevis, showed positive hybridization signals only in the NOR region in the genome of C. guttata. We demonstrated that karyotype diversification in lebiasinids was accompanied by a series of structural and numeric chromosome rearrangements of different types, including particularly fusions and fissions. © 2019 Toma et al. 2020-04-24T16:59:56Z 2020-04-24T16:59:56Z 2019 Artigo https://repositorio.inpa.gov.br/handle/1/14652 10.1371/journal.pone.0226746 en Volume 14, Número 12 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf PLoS ONE |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna 18s Dna 5s Repetitive Dna Ribosome Rna Animals Experiment C Banding Centromere Characiformes Chromosome Arm Chromosome Banding Pattern Chromosome Nor Chromosome Rearrangement Comparative Genomic Hybridization Controlled Study Copeina Guttata Cytogenetics Diploidy Dna Determination Female In Situ Hybridization, Fluorescence Giemsa Stain Heterochromatin Karyotype Lebiasina Melanoguttata Lebiasinidae Male Nonhuman Pyrrhulina Brevis Red Spotted Tetra Animals Characiformes Chromosome Classification Genetics Evolution, Molecular Phylogeny Animalss Characiformes Chromosomes Evolution, Molecular Female Karyotype Male Phylogeny Rna, Ribosomal |
| spellingShingle |
Dna 18s Dna 5s Repetitive Dna Ribosome Rna Animals Experiment C Banding Centromere Characiformes Chromosome Arm Chromosome Banding Pattern Chromosome Nor Chromosome Rearrangement Comparative Genomic Hybridization Controlled Study Copeina Guttata Cytogenetics Diploidy Dna Determination Female In Situ Hybridization, Fluorescence Giemsa Stain Heterochromatin Karyotype Lebiasina Melanoguttata Lebiasinidae Male Nonhuman Pyrrhulina Brevis Red Spotted Tetra Animals Characiformes Chromosome Classification Genetics Evolution, Molecular Phylogeny Animalss Characiformes Chromosomes Evolution, Molecular Female Karyotype Male Phylogeny Rna, Ribosomal Toma, Gustavo Akira Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
| topic_facet |
Dna 18s Dna 5s Repetitive Dna Ribosome Rna Animals Experiment C Banding Centromere Characiformes Chromosome Arm Chromosome Banding Pattern Chromosome Nor Chromosome Rearrangement Comparative Genomic Hybridization Controlled Study Copeina Guttata Cytogenetics Diploidy Dna Determination Female In Situ Hybridization, Fluorescence Giemsa Stain Heterochromatin Karyotype Lebiasina Melanoguttata Lebiasinidae Male Nonhuman Pyrrhulina Brevis Red Spotted Tetra Animals Characiformes Chromosome Classification Genetics Evolution, Molecular Phylogeny Animalss Characiformes Chromosomes Evolution, Molecular Female Karyotype Male Phylogeny Rna, Ribosomal |
| description |
Lebiasinidae is a small fish family composed by miniature to small-sized fishes with few cytogenetic data (most of them limited to descriptions of diploid chromosome numbers), thus preventing any evolutionary comparative studies at the chromosomal level. In the present study, we are providing, the first cytogenetic data for the red spotted tetra, Copeina guttata, including the standard karyotype, C-banding, repetitive DNA mapping by fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH), providing chromosomal patterns and novel insights into the karyotype differentiation of the family. Males and females share diploid chromosome number 2n = 42 and karyotype composed of 2 metacentric (m), 4 submetacentric (sm) and 36 subtelocentric to acrocentric (st-a) chromosomes. Blocks of constitutive heterochromatin were observed in the centromeric and interstitial regions of several chromosomes, in addition to a remarkably large distal block, heteromorphic in size, which fully corresponded with the 18S rDNA sites in the fourth chromosomal pair. This overlap was confirmed by 5S/18S rDNA dual-color FISH. On the other hand, 5S rDNA clusters were situated in the long and short arms of the 2nd and 15th pairs, respectively. No sex-linked karyotype differences were revealed by male/female CGH experiments. The genomic probes from other two lebiasinid species, Lebiasina melanoguttata and Pyrrhulina brevis, showed positive hybridization signals only in the NOR region in the genome of C. guttata. We demonstrated that karyotype diversification in lebiasinids was accompanied by a series of structural and numeric chromosome rearrangements of different types, including particularly fusions and fissions. © 2019 Toma et al. |
| format |
Artigo |
| author |
Toma, Gustavo Akira |
| author2 |
Moraes, Renata Luiza Rosa de Sassi, Francisco de Menezes Cavalcante Bertollo, Luiz Antônio Carlos Oliveira, Ezequiel Aguiar de Ráb, Petr Sember, Alexandr Liehr, Thomas Hatanaka, Terumi Viana, Patrik Ferreira Marinho, Manoela Maria Ferreira Feldberg, Eliana Cioffi, Marcelo de Bello |
| author2Str |
Moraes, Renata Luiza Rosa de Sassi, Francisco de Menezes Cavalcante Bertollo, Luiz Antônio Carlos Oliveira, Ezequiel Aguiar de Ráb, Petr Sember, Alexandr Liehr, Thomas Hatanaka, Terumi Viana, Patrik Ferreira Marinho, Manoela Maria Ferreira Feldberg, Eliana Cioffi, Marcelo de Bello |
| title |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
| title_short |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
| title_full |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
| title_fullStr |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
| title_full_unstemmed |
Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, Lebiasinidae): Novel insights into the karyotype differentiation of the family |
| title_sort |
cytogenetics of the small-sized fish, copeina guttata (characiformes, lebiasinidae): novel insights into the karyotype differentiation of the family |
| publisher |
PLoS ONE |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14652 |
| _version_ |
1787143168062390272 |
| score |
11.755432 |