DNA barcodes of rosy tetras and allied species (Characiformes: Characidae: Hyphessobrycon) from the Brazilian Amazon basin
DNA barcoding can be an effective tool for fast and accurate species-level identification based on sequencing of the mitochondrial cytochrome c oxidase subunit (COI) gene. The diversity of this fragment can be used to estimate the richness of the respective species. In this study, we explored the us...
| Autor principal: | Castro Paz, Francis Paola |
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| Outros Autores: | Batista, Jacqueline da Silva, Porto, Jorge Ivan Rebelo |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
PLoS ONE
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14711 |
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oai:repositorio:1-14711 |
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oai:repositorio:1-14711 DNA barcodes of rosy tetras and allied species (Characiformes: Characidae: Hyphessobrycon) from the Brazilian Amazon basin Castro Paz, Francis Paola Batista, Jacqueline da Silva Porto, Jorge Ivan Rebelo Cytochrome C Oxidase Dna Fresh Water Brasil Characidae Cladistics Coi Gene Controlled Study Dna Barcoding Freshwater Fish Genetic Variability Geographical Variation (species) Haplotype Hyphessobrycon Hyphessobrycon Bentosi Hyphessobrycon Copelandi Hyphessobrycon Epicharis Hyphessobrycon Eques Hyphessobrycon Erythrostigma Hyphessobrycon Pulchrippinis Hyphessobrycon Pyrrhonotus Hyphessobrycon Rosaceus Hyphessobrycon Socolofi Hyphessobrycon Sweglesi Neighbor Joining Method Nonhuman River Basin Species Differentiation Species Identification Animals Characidae Characiformes Dna Barcoding Fish Genetics Phylogeny Procedures River Animalss Brasil Characidae Characiformes Dna Dna Barcoding, Taxonomic Fishes Fresh Water Phylogeny Rivers DNA barcoding can be an effective tool for fast and accurate species-level identification based on sequencing of the mitochondrial cytochrome c oxidase subunit (COI) gene. The diversity of this fragment can be used to estimate the richness of the respective species. In this study, we explored the use of DNA barcoding in a group of ornamental freshwater fish of the genus Hyphessobrycon. We sequenced the COI from 10 species of Hyphessobrycon belonging to the "Rosy Tetra Clade" collected from the Amazon and Negro River basins and combined our results with published data. The average conspecific and congeneric Kimura 2-parameter distances were 2.3% and 19.3%, respectively. Six of the 10 species were easily distinguishable by DNA barcoding (H. bentosi, H. copelandi, H. eques, H. epicharis, H. pulchrippinis, and H. sweglesi), whereas the remaining species (H. erythrostigma, H. pyrrhonotus, H. rosaceus and H. socolofi) lacked reciprocal monophyly. Although the COI gene was not fully diagnostic, the discovery of distinct evolutionary units in certain Hyphessobrycon species under the same specific epithet as well as haplotype sharing between different species suggest that DNA barcoding is useful for species identification in this speciose genus. © 2014 Castro Paz et al. 2020-04-24T17:00:41Z 2020-04-24T17:00:41Z 2014 Artigo https://repositorio.inpa.gov.br/handle/1/14711 10.1371/journal.pone.0098603 en Volume 9, Número 5 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf PLoS ONE |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Cytochrome C Oxidase Dna Fresh Water Brasil Characidae Cladistics Coi Gene Controlled Study Dna Barcoding Freshwater Fish Genetic Variability Geographical Variation (species) Haplotype Hyphessobrycon Hyphessobrycon Bentosi Hyphessobrycon Copelandi Hyphessobrycon Epicharis Hyphessobrycon Eques Hyphessobrycon Erythrostigma Hyphessobrycon Pulchrippinis Hyphessobrycon Pyrrhonotus Hyphessobrycon Rosaceus Hyphessobrycon Socolofi Hyphessobrycon Sweglesi Neighbor Joining Method Nonhuman River Basin Species Differentiation Species Identification Animals Characidae Characiformes Dna Barcoding Fish Genetics Phylogeny Procedures River Animalss Brasil Characidae Characiformes Dna Dna Barcoding, Taxonomic Fishes Fresh Water Phylogeny Rivers |
| spellingShingle |
Cytochrome C Oxidase Dna Fresh Water Brasil Characidae Cladistics Coi Gene Controlled Study Dna Barcoding Freshwater Fish Genetic Variability Geographical Variation (species) Haplotype Hyphessobrycon Hyphessobrycon Bentosi Hyphessobrycon Copelandi Hyphessobrycon Epicharis Hyphessobrycon Eques Hyphessobrycon Erythrostigma Hyphessobrycon Pulchrippinis Hyphessobrycon Pyrrhonotus Hyphessobrycon Rosaceus Hyphessobrycon Socolofi Hyphessobrycon Sweglesi Neighbor Joining Method Nonhuman River Basin Species Differentiation Species Identification Animals Characidae Characiformes Dna Barcoding Fish Genetics Phylogeny Procedures River Animalss Brasil Characidae Characiformes Dna Dna Barcoding, Taxonomic Fishes Fresh Water Phylogeny Rivers Castro Paz, Francis Paola DNA barcodes of rosy tetras and allied species (Characiformes: Characidae: Hyphessobrycon) from the Brazilian Amazon basin |
| topic_facet |
Cytochrome C Oxidase Dna Fresh Water Brasil Characidae Cladistics Coi Gene Controlled Study Dna Barcoding Freshwater Fish Genetic Variability Geographical Variation (species) Haplotype Hyphessobrycon Hyphessobrycon Bentosi Hyphessobrycon Copelandi Hyphessobrycon Epicharis Hyphessobrycon Eques Hyphessobrycon Erythrostigma Hyphessobrycon Pulchrippinis Hyphessobrycon Pyrrhonotus Hyphessobrycon Rosaceus Hyphessobrycon Socolofi Hyphessobrycon Sweglesi Neighbor Joining Method Nonhuman River Basin Species Differentiation Species Identification Animals Characidae Characiformes Dna Barcoding Fish Genetics Phylogeny Procedures River Animalss Brasil Characidae Characiformes Dna Dna Barcoding, Taxonomic Fishes Fresh Water Phylogeny Rivers |
| description |
DNA barcoding can be an effective tool for fast and accurate species-level identification based on sequencing of the mitochondrial cytochrome c oxidase subunit (COI) gene. The diversity of this fragment can be used to estimate the richness of the respective species. In this study, we explored the use of DNA barcoding in a group of ornamental freshwater fish of the genus Hyphessobrycon. We sequenced the COI from 10 species of Hyphessobrycon belonging to the "Rosy Tetra Clade" collected from the Amazon and Negro River basins and combined our results with published data. The average conspecific and congeneric Kimura 2-parameter distances were 2.3% and 19.3%, respectively. Six of the 10 species were easily distinguishable by DNA barcoding (H. bentosi, H. copelandi, H. eques, H. epicharis, H. pulchrippinis, and H. sweglesi), whereas the remaining species (H. erythrostigma, H. pyrrhonotus, H. rosaceus and H. socolofi) lacked reciprocal monophyly. Although the COI gene was not fully diagnostic, the discovery of distinct evolutionary units in certain Hyphessobrycon species under the same specific epithet as well as haplotype sharing between different species suggest that DNA barcoding is useful for species identification in this speciose genus. © 2014 Castro Paz et al. |
| format |
Artigo |
| author |
Castro Paz, Francis Paola |
| author2 |
Batista, Jacqueline da Silva Porto, Jorge Ivan Rebelo |
| author2Str |
Batista, Jacqueline da Silva Porto, Jorge Ivan Rebelo |
| title |
DNA barcodes of rosy tetras and allied species (Characiformes: Characidae: Hyphessobrycon) from the Brazilian Amazon basin |
| title_short |
DNA barcodes of rosy tetras and allied species (Characiformes: Characidae: Hyphessobrycon) from the Brazilian Amazon basin |
| title_full |
DNA barcodes of rosy tetras and allied species (Characiformes: Characidae: Hyphessobrycon) from the Brazilian Amazon basin |
| title_fullStr |
DNA barcodes of rosy tetras and allied species (Characiformes: Characidae: Hyphessobrycon) from the Brazilian Amazon basin |
| title_full_unstemmed |
DNA barcodes of rosy tetras and allied species (Characiformes: Characidae: Hyphessobrycon) from the Brazilian Amazon basin |
| title_sort |
dna barcodes of rosy tetras and allied species (characiformes: characidae: hyphessobrycon) from the brazilian amazon basin |
| publisher |
PLoS ONE |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14711 |
| _version_ |
1787142253455605760 |
| score |
11.755432 |