Functional categorization of transcriptome in the species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) exposed to benzo(a)pyrene and phenanthrene
This study aims to evaluate the transcriptome alterations, through cDNA libraries, associated with the combined effects of two PAHs, benzo[a]pyrene (0.5 μg/L) and phenanthrene (50 μg/L), present in crude oil, on specimens of Symphysodon aequifasciatus (discus fish) after 48 h of exposure. The cDNA l...
| Autor principal: | Souza Pinto Lemgruber, Renato de |
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| Outros Autores: | Marshall, Nislanha Ana dos Anjos, Andrea, Ghelfi,, Fagundes, Daniel Barros, Val, Adalberto Luis |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
PLoS ONE
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14716 |
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oai:repositorio:1-14716 |
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oai:repositorio:1-14716 Functional categorization of transcriptome in the species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) exposed to benzo(a)pyrene and phenanthrene Souza Pinto Lemgruber, Renato de Marshall, Nislanha Ana dos Anjos Andrea, Ghelfi, Fagundes, Daniel Barros Val, Adalberto Luis Benzo(a)pyrene Dna, Complementary Phenanthrene Transcriptome Amino Acid Sequence Controlled Study Dna Binding Dna Library Down Regulation Environmental Exposure Gene Expression Gene Function Gene Identification Gene Interaction Gene Ontology High Throughput Sequencing Nonhuman Oxidation Reduction Reaction Perciformes Rna Extraction Serial Analysis Of Gene Expression Symphysodon Aequifasciatus Up-regulation Animalss Benzo(a)pyrene Cichlids Fish Proteins Gene Expression Regulation Phenanthrenes Transcriptome This study aims to evaluate the transcriptome alterations, through cDNA libraries, associated with the combined effects of two PAHs, benzo[a]pyrene (0.5 μg/L) and phenanthrene (50 μg/L), present in crude oil, on specimens of Symphysodon aequifasciatus (discus fish) after 48 h of exposure. The cDNA libraries were constructed according to the SOLiD™ SAGE™ protocol for sequencing in the SOLiD v.3 Plus sequencer. The results were analyzed by bioinformatics and differentially expressed genes were categorized using the gene ontology program. The functional categories (terms) found in the gene ontology and the gene network generated using STRING software were used to predict the adverse effects of benzo[a]pyrene and phenanthrene in the liver. In the present study, 27,127 genes (compared to Danio rerio database) were identified. Considering only those genes with a p-value less than or equal to 0.05 and greater than or equal to two-fold change in expression across libraries, we found 804 genes, 438 down-regulated (54%) and 366 up-regulated (46%), in the experimental group compared to the control. Out of this total, 327 genes were successfully categorized, 174 downregulated and 153 up-regulated, using gene ontology. Using String, the gene network was composed by 199 nodes, 124 of them resulting in 274 interactions. The results showed that even an acute exposure of 48 h caused metabolic change in response to environmental contaminants, resulting in changes of cell integrity, in oxidation-reduction processes, in the immune response and disturbances of intracellular signaling of discus fish. Also the gene network has showed no central interplay cluster, exhibiting instead interconnected clusters interactions and connected sub-networks. These findings highlight that even an acute sublethal exposure of PAHs can cause metabolism changes that may affect survival of discus. Our findings using SOLiD coupled with SAGE-method resulted in a powerful and reliable means for gene expression analysis in discus, a non-model Amazonian fish. © 2013 Lemgruber et al. 2020-04-24T17:00:47Z 2020-04-24T17:00:47Z 2013 Artigo https://repositorio.inpa.gov.br/handle/1/14716 10.1371/journal.pone.0081083 en Volume 8, Número 12 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf PLoS ONE |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Benzo(a)pyrene Dna, Complementary Phenanthrene Transcriptome Amino Acid Sequence Controlled Study Dna Binding Dna Library Down Regulation Environmental Exposure Gene Expression Gene Function Gene Identification Gene Interaction Gene Ontology High Throughput Sequencing Nonhuman Oxidation Reduction Reaction Perciformes Rna Extraction Serial Analysis Of Gene Expression Symphysodon Aequifasciatus Up-regulation Animalss Benzo(a)pyrene Cichlids Fish Proteins Gene Expression Regulation Phenanthrenes Transcriptome |
| spellingShingle |
Benzo(a)pyrene Dna, Complementary Phenanthrene Transcriptome Amino Acid Sequence Controlled Study Dna Binding Dna Library Down Regulation Environmental Exposure Gene Expression Gene Function Gene Identification Gene Interaction Gene Ontology High Throughput Sequencing Nonhuman Oxidation Reduction Reaction Perciformes Rna Extraction Serial Analysis Of Gene Expression Symphysodon Aequifasciatus Up-regulation Animalss Benzo(a)pyrene Cichlids Fish Proteins Gene Expression Regulation Phenanthrenes Transcriptome Souza Pinto Lemgruber, Renato de Functional categorization of transcriptome in the species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) exposed to benzo(a)pyrene and phenanthrene |
| topic_facet |
Benzo(a)pyrene Dna, Complementary Phenanthrene Transcriptome Amino Acid Sequence Controlled Study Dna Binding Dna Library Down Regulation Environmental Exposure Gene Expression Gene Function Gene Identification Gene Interaction Gene Ontology High Throughput Sequencing Nonhuman Oxidation Reduction Reaction Perciformes Rna Extraction Serial Analysis Of Gene Expression Symphysodon Aequifasciatus Up-regulation Animalss Benzo(a)pyrene Cichlids Fish Proteins Gene Expression Regulation Phenanthrenes Transcriptome |
| description |
This study aims to evaluate the transcriptome alterations, through cDNA libraries, associated with the combined effects of two PAHs, benzo[a]pyrene (0.5 μg/L) and phenanthrene (50 μg/L), present in crude oil, on specimens of Symphysodon aequifasciatus (discus fish) after 48 h of exposure. The cDNA libraries were constructed according to the SOLiD™ SAGE™ protocol for sequencing in the SOLiD v.3 Plus sequencer. The results were analyzed by bioinformatics and differentially expressed genes were categorized using the gene ontology program. The functional categories (terms) found in the gene ontology and the gene network generated using STRING software were used to predict the adverse effects of benzo[a]pyrene and phenanthrene in the liver. In the present study, 27,127 genes (compared to Danio rerio database) were identified. Considering only those genes with a p-value less than or equal to 0.05 and greater than or equal to two-fold change in expression across libraries, we found 804 genes, 438 down-regulated (54%) and 366 up-regulated (46%), in the experimental group compared to the control. Out of this total, 327 genes were successfully categorized, 174 downregulated and 153 up-regulated, using gene ontology. Using String, the gene network was composed by 199 nodes, 124 of them resulting in 274 interactions. The results showed that even an acute exposure of 48 h caused metabolic change in response to environmental contaminants, resulting in changes of cell integrity, in oxidation-reduction processes, in the immune response and disturbances of intracellular signaling of discus fish. Also the gene network has showed no central interplay cluster, exhibiting instead interconnected clusters interactions and connected sub-networks. These findings highlight that even an acute sublethal exposure of PAHs can cause metabolism changes that may affect survival of discus. Our findings using SOLiD coupled with SAGE-method resulted in a powerful and reliable means for gene expression analysis in discus, a non-model Amazonian fish. © 2013 Lemgruber et al. |
| format |
Artigo |
| author |
Souza Pinto Lemgruber, Renato de |
| author2 |
Marshall, Nislanha Ana dos Anjos Andrea, Ghelfi, Fagundes, Daniel Barros Val, Adalberto Luis |
| author2Str |
Marshall, Nislanha Ana dos Anjos Andrea, Ghelfi, Fagundes, Daniel Barros Val, Adalberto Luis |
| title |
Functional categorization of transcriptome in the species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) exposed to benzo(a)pyrene and phenanthrene |
| title_short |
Functional categorization of transcriptome in the species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) exposed to benzo(a)pyrene and phenanthrene |
| title_full |
Functional categorization of transcriptome in the species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) exposed to benzo(a)pyrene and phenanthrene |
| title_fullStr |
Functional categorization of transcriptome in the species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) exposed to benzo(a)pyrene and phenanthrene |
| title_full_unstemmed |
Functional categorization of transcriptome in the species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) exposed to benzo(a)pyrene and phenanthrene |
| title_sort |
functional categorization of transcriptome in the species symphysodon aequifasciatus pellegrin 1904 (perciformes: cichlidae) exposed to benzo(a)pyrene and phenanthrene |
| publisher |
PLoS ONE |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14716 |
| _version_ |
1787144319391498240 |
| score |
11.755432 |