Lutzomyia umbratilis, the main vector of leishmania guyanesis, represents a novel species complex?
Background: Lutzomyia umbratilis is an important Leishmania guyanensis vector in South America. Previous studies have suggested differences in the vector competence between L. umbratilis populations situated on opposite banks of the Amazonas and Negro Rivers in the central Amazonian Brazil region, l...
| Autor principal: | Scarpassa, Vera Margarete |
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| Outros Autores: | Alencar, Ronildo Baiatone |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
PLoS ONE
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14728 |
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oai:repositorio:1-14728 |
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oai:repositorio:1-14728 Lutzomyia umbratilis, the main vector of leishmania guyanesis, represents a novel species complex? Scarpassa, Vera Margarete Alencar, Ronildo Baiatone Dna, Mitochondrial Brasil Cladistics Cytochrome Oxidase Subunit I Gene Gene Gene Flow Genetic Variability Haplotype Leishmania Lutzomyia Lutzomyia Umbratilis Nonhuman Nucleotide Sequence Phylogeography Pleistocene Population Structure Riparian Ecosystem Species Differentiation Animalss Brasil Dna, Mitochondrial Gene Frequency Genetic Variation Haplotypes Insect Vectors Leishmania Guyanensis Molecular Sequence Data Phylogeny Phylogeography Psychodidae Leishmania Guyanensis Lutzomyia Umbratilis Mya Background: Lutzomyia umbratilis is an important Leishmania guyanensis vector in South America. Previous studies have suggested differences in the vector competence between L. umbratilis populations situated on opposite banks of the Amazonas and Negro Rivers in the central Amazonian Brazil region, likely indicating a species complex. However, few studies have been performed on these populations and the taxonomic status of L. umbratilis remains unclear. Methodology/Principal Findings: Phylogeographic structure was estimated for six L. umbratilis samples from the central Amazonian region in Brazil by analyzing mtDNA using 1181 bp of the COI gene to assess whether the populations on opposite banks of these rivers consist of incipient or distinct species. The genetic diversity was fairly high and the results revealed two distinct clades (= lineages) with 1% sequence divergence. Clade I consisted of four samples from the left bank of the Amazonas and Negro Rivers, whereas clade II comprised two samples from the right bank of Negro River. No haplotypes were shared between samples of two clades. Samples within clades exhibited low to moderate genetic differentiation (FST = -0.0390-0.1841), whereas samples between clades exhibited very high differentiation (FST = 0.7100-0.8497) and fixed differences. These lineages have diverged approximately 0.22 Mya in the middle Pleistocene. Demographic expansion was detected for the lineages I and II approximately 30,448 and 15,859 years ago, respectively, in the late Pleistocene. Conclusions/Significance: The two genetic lineages may represent an advanced speciation stage suggestive of incipient or distinct species within L. umbratilis. These findings suggest that the Amazonas and Negro Rivers may be acting as effective barriers, thus preventing gene flow between populations on opposite sides. Such findings have important implications for epidemiological studies, especially those related to vector competence and anthropophily, and for vector control strategies. In addition, L. umbratilis represents an interesting example in speciation studies. © 2012 Scarpassa, Alencar. 2020-04-24T17:01:00Z 2020-04-24T17:01:00Z 2012 Artigo https://repositorio.inpa.gov.br/handle/1/14728 10.1371/journal.pone.0037341 en Volume 7, Número 5 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf PLoS ONE |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna, Mitochondrial Brasil Cladistics Cytochrome Oxidase Subunit I Gene Gene Gene Flow Genetic Variability Haplotype Leishmania Lutzomyia Lutzomyia Umbratilis Nonhuman Nucleotide Sequence Phylogeography Pleistocene Population Structure Riparian Ecosystem Species Differentiation Animalss Brasil Dna, Mitochondrial Gene Frequency Genetic Variation Haplotypes Insect Vectors Leishmania Guyanensis Molecular Sequence Data Phylogeny Phylogeography Psychodidae Leishmania Guyanensis Lutzomyia Umbratilis Mya |
| spellingShingle |
Dna, Mitochondrial Brasil Cladistics Cytochrome Oxidase Subunit I Gene Gene Gene Flow Genetic Variability Haplotype Leishmania Lutzomyia Lutzomyia Umbratilis Nonhuman Nucleotide Sequence Phylogeography Pleistocene Population Structure Riparian Ecosystem Species Differentiation Animalss Brasil Dna, Mitochondrial Gene Frequency Genetic Variation Haplotypes Insect Vectors Leishmania Guyanensis Molecular Sequence Data Phylogeny Phylogeography Psychodidae Leishmania Guyanensis Lutzomyia Umbratilis Mya Scarpassa, Vera Margarete Lutzomyia umbratilis, the main vector of leishmania guyanesis, represents a novel species complex? |
| topic_facet |
Dna, Mitochondrial Brasil Cladistics Cytochrome Oxidase Subunit I Gene Gene Gene Flow Genetic Variability Haplotype Leishmania Lutzomyia Lutzomyia Umbratilis Nonhuman Nucleotide Sequence Phylogeography Pleistocene Population Structure Riparian Ecosystem Species Differentiation Animalss Brasil Dna, Mitochondrial Gene Frequency Genetic Variation Haplotypes Insect Vectors Leishmania Guyanensis Molecular Sequence Data Phylogeny Phylogeography Psychodidae Leishmania Guyanensis Lutzomyia Umbratilis Mya |
| description |
Background: Lutzomyia umbratilis is an important Leishmania guyanensis vector in South America. Previous studies have suggested differences in the vector competence between L. umbratilis populations situated on opposite banks of the Amazonas and Negro Rivers in the central Amazonian Brazil region, likely indicating a species complex. However, few studies have been performed on these populations and the taxonomic status of L. umbratilis remains unclear. Methodology/Principal Findings: Phylogeographic structure was estimated for six L. umbratilis samples from the central Amazonian region in Brazil by analyzing mtDNA using 1181 bp of the COI gene to assess whether the populations on opposite banks of these rivers consist of incipient or distinct species. The genetic diversity was fairly high and the results revealed two distinct clades (= lineages) with 1% sequence divergence. Clade I consisted of four samples from the left bank of the Amazonas and Negro Rivers, whereas clade II comprised two samples from the right bank of Negro River. No haplotypes were shared between samples of two clades. Samples within clades exhibited low to moderate genetic differentiation (FST = -0.0390-0.1841), whereas samples between clades exhibited very high differentiation (FST = 0.7100-0.8497) and fixed differences. These lineages have diverged approximately 0.22 Mya in the middle Pleistocene. Demographic expansion was detected for the lineages I and II approximately 30,448 and 15,859 years ago, respectively, in the late Pleistocene. Conclusions/Significance: The two genetic lineages may represent an advanced speciation stage suggestive of incipient or distinct species within L. umbratilis. These findings suggest that the Amazonas and Negro Rivers may be acting as effective barriers, thus preventing gene flow between populations on opposite sides. Such findings have important implications for epidemiological studies, especially those related to vector competence and anthropophily, and for vector control strategies. In addition, L. umbratilis represents an interesting example in speciation studies. © 2012 Scarpassa, Alencar. |
| format |
Artigo |
| author |
Scarpassa, Vera Margarete |
| author2 |
Alencar, Ronildo Baiatone |
| author2Str |
Alencar, Ronildo Baiatone |
| title |
Lutzomyia umbratilis, the main vector of leishmania guyanesis, represents a novel species complex? |
| title_short |
Lutzomyia umbratilis, the main vector of leishmania guyanesis, represents a novel species complex? |
| title_full |
Lutzomyia umbratilis, the main vector of leishmania guyanesis, represents a novel species complex? |
| title_fullStr |
Lutzomyia umbratilis, the main vector of leishmania guyanesis, represents a novel species complex? |
| title_full_unstemmed |
Lutzomyia umbratilis, the main vector of leishmania guyanesis, represents a novel species complex? |
| title_sort |
lutzomyia umbratilis, the main vector of leishmania guyanesis, represents a novel species complex? |
| publisher |
PLoS ONE |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14728 |
| _version_ |
1787144319540396032 |
| score |
11.755432 |