Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNγ production
IL-23/IL-17-induced neutrophil recruitment plays a pivotal role in rheumatoid arthritis (RA). However, the mechanism of the neutrophil recruitment is obscure. Here we report that prostaglandin enhances the IL-23/IL-17-induced neutrophil migration in a murine model of RA by inhibiting IL-12 and IFN γ...
| Autor principal: | Lemos, Henrique Paula |
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| Outros Autores: | Grespan, Renata, Manfredo Vieira, Silvio, Cunha, Thiago Mattar, Verri, Waldiceu A., Fernandes, Karla S., Souto, Fabrício Oliveira, McInnes, Iain B., Ferreira, Seérgio Henrique, Liew, Foo Y., Cunha, Fernando Queiroz |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Proceedings of the National Academy of Sciences of the United States of America
2020
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https://repositorio.inpa.gov.br/handle/1/14856 |
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oai:repositorio:1-14856 Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNγ production Lemos, Henrique Paula Grespan, Renata Manfredo Vieira, Silvio Cunha, Thiago Mattar Verri, Waldiceu A. Fernandes, Karla S. Souto, Fabrício Oliveira McInnes, Iain B. Ferreira, Seérgio Henrique Liew, Foo Y. Cunha, Fernando Queiroz 3 [3 Tert Butylthio 1 (4 Chlorobenzyl) 5 Isopropyl 2 Indolyl] 2,2 Dimethylpropionic Acid Etoricoxib Gamma Interferon Indometacin Interleukin-12 Interleukin-17 Interleukin-23 Prostaglandin Prostaglandin Synthase Inhibitor Tumor Necrosis Factor Alpha Antibody Animals Cell Animals Experiment Animals Model Animals Tissue Arthritis Cell Migration Controlled Study Female Male Mouse Neutrophil Neutrophil Chemotaxis Nonhuman Pathogenesis Priority Journal Protein Expression Protein Function Animal Arthritis, Rheumatoid Cyclooxygenase Inhibitors Dinoprostone Inflammation Interferon-gamma Interleukin-12 Interleukin-17 Interleukin-23 Mice Neutrophil Infiltration Prostaglandins Murinae Mus IL-23/IL-17-induced neutrophil recruitment plays a pivotal role in rheumatoid arthritis (RA). However, the mechanism of the neutrophil recruitment is obscure. Here we report that prostaglandin enhances the IL-23/IL-17-induced neutrophil migration in a murine model of RA by inhibiting IL-12 and IFN γ production. Methylated BSA (mBSA) and IL-23-induced neutrophil migration was inhibited by anti-IL-23 and anti-IL-17 antibodies, COX inhibitors, IL-12, or IFNy but was enhanced by prostaglandin E2 (PGE2). IL-23-induced IL-17 production was increased by PGE2 and suppressed by COX- inhibition or IL-12. Furthermore, COX inhibition failed to reduce IL-23-induced neutrophil migration in IL-12- or IFNγ-deficient mice. IL-17-induced neutrophil migration was not affected by COX inhibitors, IL-12, or IFNγ but was inhibited by MK886 (a leukotriene synthesis inhibitor), anti-TNFα, anti-CXCL1, and anti-CXCL5 antibodies and by repertaxin (a CXCR1/2 antagonist). These treatments all inhibited mBSA- or IL-23-induced neutrophil migration. IL-17 induced neutrophil chemotaxis through a CXC chemokines-dependent pathway. Our results suggest that prostaglandin plays an important role in IL-23-induced neutrophil migration in arthritis by enhancing IL-17 synthesis and by inhibiting IL-12 and IFNγ production. We thus provide a mechanism for the pathogenic role of the IL-23/IL-17 axis in RA and also suggest an additional mechanism of action for nonsteroidal anti-inflammatory drugs. 2020-05-07T13:41:03Z 2020-05-07T13:41:03Z 2009 Artigo https://repositorio.inpa.gov.br/handle/1/14856 10.1073/pnas.0812782106 en Volume 106, Número 14, Pags. 5954-5959 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Proceedings of the National Academy of Sciences of the United States of America |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
3 [3 Tert Butylthio 1 (4 Chlorobenzyl) 5 Isopropyl 2 Indolyl] 2,2 Dimethylpropionic Acid Etoricoxib Gamma Interferon Indometacin Interleukin-12 Interleukin-17 Interleukin-23 Prostaglandin Prostaglandin Synthase Inhibitor Tumor Necrosis Factor Alpha Antibody Animals Cell Animals Experiment Animals Model Animals Tissue Arthritis Cell Migration Controlled Study Female Male Mouse Neutrophil Neutrophil Chemotaxis Nonhuman Pathogenesis Priority Journal Protein Expression Protein Function Animal Arthritis, Rheumatoid Cyclooxygenase Inhibitors Dinoprostone Inflammation Interferon-gamma Interleukin-12 Interleukin-17 Interleukin-23 Mice Neutrophil Infiltration Prostaglandins Murinae Mus |
| spellingShingle |
3 [3 Tert Butylthio 1 (4 Chlorobenzyl) 5 Isopropyl 2 Indolyl] 2,2 Dimethylpropionic Acid Etoricoxib Gamma Interferon Indometacin Interleukin-12 Interleukin-17 Interleukin-23 Prostaglandin Prostaglandin Synthase Inhibitor Tumor Necrosis Factor Alpha Antibody Animals Cell Animals Experiment Animals Model Animals Tissue Arthritis Cell Migration Controlled Study Female Male Mouse Neutrophil Neutrophil Chemotaxis Nonhuman Pathogenesis Priority Journal Protein Expression Protein Function Animal Arthritis, Rheumatoid Cyclooxygenase Inhibitors Dinoprostone Inflammation Interferon-gamma Interleukin-12 Interleukin-17 Interleukin-23 Mice Neutrophil Infiltration Prostaglandins Murinae Mus Lemos, Henrique Paula Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNγ production |
| topic_facet |
3 [3 Tert Butylthio 1 (4 Chlorobenzyl) 5 Isopropyl 2 Indolyl] 2,2 Dimethylpropionic Acid Etoricoxib Gamma Interferon Indometacin Interleukin-12 Interleukin-17 Interleukin-23 Prostaglandin Prostaglandin Synthase Inhibitor Tumor Necrosis Factor Alpha Antibody Animals Cell Animals Experiment Animals Model Animals Tissue Arthritis Cell Migration Controlled Study Female Male Mouse Neutrophil Neutrophil Chemotaxis Nonhuman Pathogenesis Priority Journal Protein Expression Protein Function Animal Arthritis, Rheumatoid Cyclooxygenase Inhibitors Dinoprostone Inflammation Interferon-gamma Interleukin-12 Interleukin-17 Interleukin-23 Mice Neutrophil Infiltration Prostaglandins Murinae Mus |
| description |
IL-23/IL-17-induced neutrophil recruitment plays a pivotal role in rheumatoid arthritis (RA). However, the mechanism of the neutrophil recruitment is obscure. Here we report that prostaglandin enhances the IL-23/IL-17-induced neutrophil migration in a murine model of RA by inhibiting IL-12 and IFN γ production. Methylated BSA (mBSA) and IL-23-induced neutrophil migration was inhibited by anti-IL-23 and anti-IL-17 antibodies, COX inhibitors, IL-12, or IFNy but was enhanced by prostaglandin E2 (PGE2). IL-23-induced IL-17 production was increased by PGE2 and suppressed by COX- inhibition or IL-12. Furthermore, COX inhibition failed to reduce IL-23-induced neutrophil migration in IL-12- or IFNγ-deficient mice. IL-17-induced neutrophil migration was not affected by COX inhibitors, IL-12, or IFNγ but was inhibited by MK886 (a leukotriene synthesis inhibitor), anti-TNFα, anti-CXCL1, and anti-CXCL5 antibodies and by repertaxin (a CXCR1/2 antagonist). These treatments all inhibited mBSA- or IL-23-induced neutrophil migration. IL-17 induced neutrophil chemotaxis through a CXC chemokines-dependent pathway. Our results suggest that prostaglandin plays an important role in IL-23-induced neutrophil migration in arthritis by enhancing IL-17 synthesis and by inhibiting IL-12 and IFNγ production. We thus provide a mechanism for the pathogenic role of the IL-23/IL-17 axis in RA and also suggest an additional mechanism of action for nonsteroidal anti-inflammatory drugs. |
| format |
Artigo |
| author |
Lemos, Henrique Paula |
| author2 |
Grespan, Renata Manfredo Vieira, Silvio Cunha, Thiago Mattar Verri, Waldiceu A. Fernandes, Karla S. Souto, Fabrício Oliveira McInnes, Iain B. Ferreira, Seérgio Henrique Liew, Foo Y. Cunha, Fernando Queiroz |
| author2Str |
Grespan, Renata Manfredo Vieira, Silvio Cunha, Thiago Mattar Verri, Waldiceu A. Fernandes, Karla S. Souto, Fabrício Oliveira McInnes, Iain B. Ferreira, Seérgio Henrique Liew, Foo Y. Cunha, Fernando Queiroz |
| title |
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNγ production |
| title_short |
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNγ production |
| title_full |
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNγ production |
| title_fullStr |
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNγ production |
| title_full_unstemmed |
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNγ production |
| title_sort |
prostaglandin mediates il-23/il-17-induced neutrophil migration in inflammation by inhibiting il-12 and ifnγ production |
| publisher |
Proceedings of the National Academy of Sciences of the United States of America |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14856 |
| _version_ |
1787143325336207360 |
| score |
11.653393 |