Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such aserythropoietin,transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the...
| Autor principal: | Almeida-Val, Vera Maria Fonseca |
|---|---|
| Outros Autores: | Oliveira, Alice Reis, Silva, Maria de Nazaré Paula da, Ferreira-Nozawa, Mônica Stropa, Araújo, Roziete Mendes, Val, Adalberto Luis, Nozawa, Sérgio Ricardo |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Biology
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14955 |
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oai:repositorio:1-14955 |
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dspace |
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oai:repositorio:1-14955 Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis Almeida-Val, Vera Maria Fonseca Oliveira, Alice Reis Silva, Maria de Nazaré Paula da Ferreira-Nozawa, Mônica Stropa Araújo, Roziete Mendes Val, Adalberto Luis Nozawa, Sérgio Ricardo Lactate Dehydrogenase Lactate Dehydrogenase Isoenzyme 5 Rna, Messenger Unclassified Drug Acclimatization Age Animals Experiment Animals Tissue Anoxia Astronotus Crassipinis Brasil Controlled Study Fish Gene Expression Gene Sequence Heart Muscle Hypoxia Ldh A Gene Cloning, Molecular Nonhuman Nucleotide Sequence Muscle, Skeletal Astronotus Pisces Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such aserythropoietin,transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis(the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A expression. In adults, the levels of LDH-A mRNA did not increase in hypoxic or anoxic conditions. Our results demonstrate that, when given time for acclimation, fish at different life-stages are able to respond differently to survive hypoxic episodes. © 2011, Sociedade Brasileira de Genética. 2020-05-07T13:59:06Z 2020-05-07T13:59:06Z 2011 Artigo https://repositorio.inpa.gov.br/handle/1/14955 10.1590/S1415-47572011000200025 en Volume 34, Número 2, Pags. 315-322 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Biology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Lactate Dehydrogenase Lactate Dehydrogenase Isoenzyme 5 Rna, Messenger Unclassified Drug Acclimatization Age Animals Experiment Animals Tissue Anoxia Astronotus Crassipinis Brasil Controlled Study Fish Gene Expression Gene Sequence Heart Muscle Hypoxia Ldh A Gene Cloning, Molecular Nonhuman Nucleotide Sequence Muscle, Skeletal Astronotus Pisces |
| spellingShingle |
Lactate Dehydrogenase Lactate Dehydrogenase Isoenzyme 5 Rna, Messenger Unclassified Drug Acclimatization Age Animals Experiment Animals Tissue Anoxia Astronotus Crassipinis Brasil Controlled Study Fish Gene Expression Gene Sequence Heart Muscle Hypoxia Ldh A Gene Cloning, Molecular Nonhuman Nucleotide Sequence Muscle, Skeletal Astronotus Pisces Almeida-Val, Vera Maria Fonseca Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis |
| topic_facet |
Lactate Dehydrogenase Lactate Dehydrogenase Isoenzyme 5 Rna, Messenger Unclassified Drug Acclimatization Age Animals Experiment Animals Tissue Anoxia Astronotus Crassipinis Brasil Controlled Study Fish Gene Expression Gene Sequence Heart Muscle Hypoxia Ldh A Gene Cloning, Molecular Nonhuman Nucleotide Sequence Muscle, Skeletal Astronotus Pisces |
| description |
Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such aserythropoietin,transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis(the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A expression. In adults, the levels of LDH-A mRNA did not increase in hypoxic or anoxic conditions. Our results demonstrate that, when given time for acclimation, fish at different life-stages are able to respond differently to survive hypoxic episodes. © 2011, Sociedade Brasileira de Genética. |
| format |
Artigo |
| author |
Almeida-Val, Vera Maria Fonseca |
| author2 |
Oliveira, Alice Reis Silva, Maria de Nazaré Paula da Ferreira-Nozawa, Mônica Stropa Araújo, Roziete Mendes Val, Adalberto Luis Nozawa, Sérgio Ricardo |
| author2Str |
Oliveira, Alice Reis Silva, Maria de Nazaré Paula da Ferreira-Nozawa, Mônica Stropa Araújo, Roziete Mendes Val, Adalberto Luis Nozawa, Sérgio Ricardo |
| title |
Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis |
| title_short |
Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis |
| title_full |
Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis |
| title_fullStr |
Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis |
| title_full_unstemmed |
Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis |
| title_sort |
anoxia- and hypoxia-induced expression of ldh-a* in the amazon oscar, astronotus crassipinis |
| publisher |
Genetics and Molecular Biology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14955 |
| _version_ |
1787142413280608256 |
| score |
11.675088 |