Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae)
We report the characterization and optimization of 45 heterologous microsatellite loci, and the development of a new set of molecular sex markers for the conservation and management of the Neotropical harpy eagle (Harpia harpyja L. 1758). Of the 45 microsatellites tested, 24 were polymorphic, six mo...
| Autor principal: | Banhos, Aureo |
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| Outros Autores: | Hrbek, Tomas, Gravena, Waleska, Sanaiotti, Tânia Margarete, Farias, Izeni P. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Biology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14961 |
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oai:repositorio:1-14961 |
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oai:repositorio:1-14961 Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae) Banhos, Aureo Hrbek, Tomas Gravena, Waleska Sanaiotti, Tânia Margarete Farias, Izeni P. Genomic Dna Animals Tissue Conservation Genetics Controlled Study Dna Degradation Dna Extraction Eagle Falconiformes Feather Female Gene Amplification Gene Locus Polymorphism, Genetic Genetic Variability Genomics Male Microsatellite Marker Nonhuman Nucleotide Sequence Paternity Probability Sex Determination Species Conservations Statistical Significance Wild Species Accipitridae Falconiformes Harpia Harpyja Morphnus Guianensis Raptores We report the characterization and optimization of 45 heterologous microsatellite loci, and the development of a new set of molecular sex markers for the conservation and management of the Neotropical harpy eagle (Harpia harpyja L. 1758). Of the 45 microsatellites tested, 24 were polymorphic, six monomorphic, 10 uncharacterizable due to multiple bands and five did not amplify. The observed gene diversity of the analyzed sample of H. harpyja was low and similar to that of other threatened Falconiformes. While a high proportion of the microsatellite markers were highly variable, individuals of H. harpyja could be differentiated by a joint analysis of just three (p = 2.79 × 10-4) or four markers (p = 2.89 × 10-4). Paternity could be rejected with 95.23% and 97.83% probabilities using the same three and four markers, respectively. The sex determination markers easily and consistently differentiated males from females even with highly degraded DNA extracted from naturally shed feathers. The markers reported in this study potentially provide an excellent set of molecular tools for the conservation and management of wild and captive H. harpyja and they may also prove useful for the enigmatic Neotropical crested eagle (Morphnus guianensis Daudin 1800). Copyright by the Brazilian Society of Genetics. 2020-05-07T13:59:09Z 2020-05-07T13:59:09Z 2008 Artigo https://repositorio.inpa.gov.br/handle/1/14961 10.1590/S1415-47572008000100025 en Volume 31, Número 1, Pags. 146-154 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Biology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Genomic Dna Animals Tissue Conservation Genetics Controlled Study Dna Degradation Dna Extraction Eagle Falconiformes Feather Female Gene Amplification Gene Locus Polymorphism, Genetic Genetic Variability Genomics Male Microsatellite Marker Nonhuman Nucleotide Sequence Paternity Probability Sex Determination Species Conservations Statistical Significance Wild Species Accipitridae Falconiformes Harpia Harpyja Morphnus Guianensis Raptores |
| spellingShingle |
Genomic Dna Animals Tissue Conservation Genetics Controlled Study Dna Degradation Dna Extraction Eagle Falconiformes Feather Female Gene Amplification Gene Locus Polymorphism, Genetic Genetic Variability Genomics Male Microsatellite Marker Nonhuman Nucleotide Sequence Paternity Probability Sex Determination Species Conservations Statistical Significance Wild Species Accipitridae Falconiformes Harpia Harpyja Morphnus Guianensis Raptores Banhos, Aureo Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae) |
| topic_facet |
Genomic Dna Animals Tissue Conservation Genetics Controlled Study Dna Degradation Dna Extraction Eagle Falconiformes Feather Female Gene Amplification Gene Locus Polymorphism, Genetic Genetic Variability Genomics Male Microsatellite Marker Nonhuman Nucleotide Sequence Paternity Probability Sex Determination Species Conservations Statistical Significance Wild Species Accipitridae Falconiformes Harpia Harpyja Morphnus Guianensis Raptores |
| description |
We report the characterization and optimization of 45 heterologous microsatellite loci, and the development of a new set of molecular sex markers for the conservation and management of the Neotropical harpy eagle (Harpia harpyja L. 1758). Of the 45 microsatellites tested, 24 were polymorphic, six monomorphic, 10 uncharacterizable due to multiple bands and five did not amplify. The observed gene diversity of the analyzed sample of H. harpyja was low and similar to that of other threatened Falconiformes. While a high proportion of the microsatellite markers were highly variable, individuals of H. harpyja could be differentiated by a joint analysis of just three (p = 2.79 × 10-4) or four markers (p = 2.89 × 10-4). Paternity could be rejected with 95.23% and 97.83% probabilities using the same three and four markers, respectively. The sex determination markers easily and consistently differentiated males from females even with highly degraded DNA extracted from naturally shed feathers. The markers reported in this study potentially provide an excellent set of molecular tools for the conservation and management of wild and captive H. harpyja and they may also prove useful for the enigmatic Neotropical crested eagle (Morphnus guianensis Daudin 1800). Copyright by the Brazilian Society of Genetics. |
| format |
Artigo |
| author |
Banhos, Aureo |
| author2 |
Hrbek, Tomas Gravena, Waleska Sanaiotti, Tânia Margarete Farias, Izeni P. |
| author2Str |
Hrbek, Tomas Gravena, Waleska Sanaiotti, Tânia Margarete Farias, Izeni P. |
| title |
Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae) |
| title_short |
Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae) |
| title_full |
Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae) |
| title_fullStr |
Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae) |
| title_full_unstemmed |
Genomic resources for the conservation and management of the harpy eagle (Harpia harpyja, Falconiformes, Accipitridae) |
| title_sort |
genomic resources for the conservation and management of the harpy eagle (harpia harpyja, falconiformes, accipitridae) |
| publisher |
Genetics and Molecular Biology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14961 |
| _version_ |
1787142256375889920 |
| score |
11.755432 |