Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum
Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis a...
| Autor principal: | Cordeiro, Isabelle Bezerra |
|---|---|
| Outros Autores: | Castro, Diogo Pereira de, Nogueira, P. P O, Angelo, P., Nogueira, Paulo Afonso, Gonçalves, José Francisco Carvalho de, Pereira, A. M.R.F., Garcia, Jerusa Simone, Souza, Gustavo H.M.F., Arruda, Marco Aurélio Zezzi, Eberlin, M. N., Astolfi-Filho, Spártaco A.T., Andrade, E. V. de, López-Lozano, Jorge Luis |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/14992 |
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oai:repositorio:1-14992 |
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oai:repositorio:1-14992 Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum Cordeiro, Isabelle Bezerra Castro, Diogo Pereira de Nogueira, P. P O Angelo, P. Nogueira, Paulo Afonso Gonçalves, José Francisco Carvalho de Pereira, A. M.R.F. Garcia, Jerusa Simone Souza, Gustavo H.M.F. Arruda, Marco Aurélio Zezzi Eberlin, M. N. Astolfi-Filho, Spártaco A.T. Andrade, E. V. de López-Lozano, Jorge Luis Bacterial Protein Carrier Protein Chaperone Elongation Factor Elongation Factor P Membrane Protein Ribosome Protein Sigma Factor Sigma Factor 32 Small Heat Shock Protein Superoxide Dismutase Unclassified Drug Bacterial Protein Chaperone Bacterial Growth Chromobacterium Violaceum Electrophoresis Heat Acclimatization Heat-shock Response Heat Stress Inverted Repeat Mass Spectrometry Nonhuman Promoter Region Protein Expression Proteomics Adaptation Cell Respiration Chromobacterium Fermentation Gene Expression Regulation Genetics Growth, Development And Aging Metabolism Open Reading Frame Stress, Physiological Procedures Proteomics Temperature Bacteria (microorganisms) Chromobacterium Violaceum Negibacteria Proteobacteria Adaptation, Biological Bacterial Proteins Cell Respiration Chromobacterium Fermentation Gene Expression Regulation, Bacterial Molecular Chaperones Open Reading Frames Promoter Regions, Genetic Proteomics Stress, Physiological Temperature Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected was 1985; the number ranged from 99 to 380 in each assay. The proteins that were identified spectrometrically were categorized as chaperones, proteins expressed exclusively under heat stress, enzymes involved in the respiratory and fermentation cycles, ribosomal proteins, and proteins related to transport and secretion. Controlling inverted repeat of chaperone expression and inverted repeat DNA binding sequences, as well as regions recognized by sigma factor 32, elements involved in the genetic regulation of the bacterial stress response, were identified in the promoter regions of several of the genes coding proteins, involved in the C. violaceum stress response. We found that 30°C is the optimal growth temperature for C. violaceum, whereas 25, 35, and 40°C are stressful temperatures that trigger the expression of chaperones, superoxide dismutase, a probable small heat shock protein, a probable phasing, ferrichrome-iron receptor protein, elongation factor P, and an ornithine carbamoyltransferase catabolite. This information improves our comprehension of the mechanisms involved in stress adaptation by C. violaceum. © FUNPEC-RP. 2020-05-07T14:00:23Z 2020-05-07T14:00:23Z 2013 Artigo https://repositorio.inpa.gov.br/handle/1/14992 10.4238/2013.October.29.1 en Volume 12, Número 4, Pags. 5057-5071 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Bacterial Protein Carrier Protein Chaperone Elongation Factor Elongation Factor P Membrane Protein Ribosome Protein Sigma Factor Sigma Factor 32 Small Heat Shock Protein Superoxide Dismutase Unclassified Drug Bacterial Protein Chaperone Bacterial Growth Chromobacterium Violaceum Electrophoresis Heat Acclimatization Heat-shock Response Heat Stress Inverted Repeat Mass Spectrometry Nonhuman Promoter Region Protein Expression Proteomics Adaptation Cell Respiration Chromobacterium Fermentation Gene Expression Regulation Genetics Growth, Development And Aging Metabolism Open Reading Frame Stress, Physiological Procedures Proteomics Temperature Bacteria (microorganisms) Chromobacterium Violaceum Negibacteria Proteobacteria Adaptation, Biological Bacterial Proteins Cell Respiration Chromobacterium Fermentation Gene Expression Regulation, Bacterial Molecular Chaperones Open Reading Frames Promoter Regions, Genetic Proteomics Stress, Physiological Temperature |
| spellingShingle |
Bacterial Protein Carrier Protein Chaperone Elongation Factor Elongation Factor P Membrane Protein Ribosome Protein Sigma Factor Sigma Factor 32 Small Heat Shock Protein Superoxide Dismutase Unclassified Drug Bacterial Protein Chaperone Bacterial Growth Chromobacterium Violaceum Electrophoresis Heat Acclimatization Heat-shock Response Heat Stress Inverted Repeat Mass Spectrometry Nonhuman Promoter Region Protein Expression Proteomics Adaptation Cell Respiration Chromobacterium Fermentation Gene Expression Regulation Genetics Growth, Development And Aging Metabolism Open Reading Frame Stress, Physiological Procedures Proteomics Temperature Bacteria (microorganisms) Chromobacterium Violaceum Negibacteria Proteobacteria Adaptation, Biological Bacterial Proteins Cell Respiration Chromobacterium Fermentation Gene Expression Regulation, Bacterial Molecular Chaperones Open Reading Frames Promoter Regions, Genetic Proteomics Stress, Physiological Temperature Cordeiro, Isabelle Bezerra Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum |
| topic_facet |
Bacterial Protein Carrier Protein Chaperone Elongation Factor Elongation Factor P Membrane Protein Ribosome Protein Sigma Factor Sigma Factor 32 Small Heat Shock Protein Superoxide Dismutase Unclassified Drug Bacterial Protein Chaperone Bacterial Growth Chromobacterium Violaceum Electrophoresis Heat Acclimatization Heat-shock Response Heat Stress Inverted Repeat Mass Spectrometry Nonhuman Promoter Region Protein Expression Proteomics Adaptation Cell Respiration Chromobacterium Fermentation Gene Expression Regulation Genetics Growth, Development And Aging Metabolism Open Reading Frame Stress, Physiological Procedures Proteomics Temperature Bacteria (microorganisms) Chromobacterium Violaceum Negibacteria Proteobacteria Adaptation, Biological Bacterial Proteins Cell Respiration Chromobacterium Fermentation Gene Expression Regulation, Bacterial Molecular Chaperones Open Reading Frames Promoter Regions, Genetic Proteomics Stress, Physiological Temperature |
| description |
Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected was 1985; the number ranged from 99 to 380 in each assay. The proteins that were identified spectrometrically were categorized as chaperones, proteins expressed exclusively under heat stress, enzymes involved in the respiratory and fermentation cycles, ribosomal proteins, and proteins related to transport and secretion. Controlling inverted repeat of chaperone expression and inverted repeat DNA binding sequences, as well as regions recognized by sigma factor 32, elements involved in the genetic regulation of the bacterial stress response, were identified in the promoter regions of several of the genes coding proteins, involved in the C. violaceum stress response. We found that 30°C is the optimal growth temperature for C. violaceum, whereas 25, 35, and 40°C are stressful temperatures that trigger the expression of chaperones, superoxide dismutase, a probable small heat shock protein, a probable phasing, ferrichrome-iron receptor protein, elongation factor P, and an ornithine carbamoyltransferase catabolite. This information improves our comprehension of the mechanisms involved in stress adaptation by C. violaceum. © FUNPEC-RP. |
| format |
Artigo |
| author |
Cordeiro, Isabelle Bezerra |
| author2 |
Castro, Diogo Pereira de Nogueira, P. P O Angelo, P. Nogueira, Paulo Afonso Gonçalves, José Francisco Carvalho de Pereira, A. M.R.F. Garcia, Jerusa Simone Souza, Gustavo H.M.F. Arruda, Marco Aurélio Zezzi Eberlin, M. N. Astolfi-Filho, Spártaco A.T. Andrade, E. V. de López-Lozano, Jorge Luis |
| author2Str |
Castro, Diogo Pereira de Nogueira, P. P O Angelo, P. Nogueira, Paulo Afonso Gonçalves, José Francisco Carvalho de Pereira, A. M.R.F. Garcia, Jerusa Simone Souza, Gustavo H.M.F. Arruda, Marco Aurélio Zezzi Eberlin, M. N. Astolfi-Filho, Spártaco A.T. Andrade, E. V. de López-Lozano, Jorge Luis |
| title |
Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum |
| title_short |
Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum |
| title_full |
Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum |
| title_fullStr |
Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum |
| title_full_unstemmed |
Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum |
| title_sort |
electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed chromobacterium violaceum |
| publisher |
Genetics and Molecular Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/14992 |
| _version_ |
1787145415382007808 |
| score |
11.755432 |