Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene
Aedes albopictus, a mosquito originally from Southeast Asia, is considered to be one of the main vectors of dengue fever, yellow fever and other arboviruses. We examined the genetic variability and population structure of 68 individuals of Ae. albopictus collected from five neighborhoods of the city...
| Autor principal: | Maia, R. T. |
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| Outros Autores: | Scarpassa, Vera Margarete, Maciel-Litaiff, L. H., Tadei, Wanderli Pedro |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Research
2020
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https://repositorio.inpa.gov.br/handle/1/15000 |
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oai:repositorio:1-15000 |
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oai:repositorio:1-15000 Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene Maia, R. T. Scarpassa, Vera Margarete Maciel-Litaiff, L. H. Tadei, Wanderli Pedro Dna, Mitochondrial Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase Subunit 5 Unclassified Drug Aedes Albopictus Asia Brasil Controlled Study Dna Determination Gene Structures Genetic Linkage Genetic Variability Haplotype Mutational Analysis Nonhuman Nucleotide Sequence Genetics, Population Population Structure Structure Analysis United States Aedes Animal Brasil Dna, Mitochondrial Gene Flow Genes, Insect Genes, Mitochondrial Genetic Variation Geography Haplotypes Aedes Albopictus Arbovirus Culicidae Diptera Aedes albopictus, a mosquito originally from Southeast Asia, is considered to be one of the main vectors of dengue fever, yellow fever and other arboviruses. We examined the genetic variability and population structure of 68 individuals of Ae. albopictus collected from five neighborhoods of the city of Manaus, based on the mitochondrial gene coding for NADH dehydrogenase subunit 5 (ND5). Two haplotypes were found, separated by a single mutational event (T ↔ C), with extremely low levels of genetic variability (h = 0.187 ± 0.059; π = 0.00044 ± 0.00014). Based on AMOVA, we concluded that most of the variation (99.08%) occurred within populations, though the levels of variation were not significant. Neutrality tests (Tajima's D and Fu's Fs) were non-significant, indicating that these populations are in genetic equilibrium. The most frequent haplotype (H1) is restricted to Brazilian populations of Ae. albopictus, while the rarer haplotype (H2) is shared with populations from the United States and Asia. We suggest that the reduced variability and low genetic structure identified in our study is a consequence of the recent introduction of this species in Manaus, possibly through a founder effect, followed by expansion throughout the city neighborhoods. Genetic similarity would therefore be due to insufficient time to have accumulated genetic differences between the populations of Ae. albopictus and not to extensive gene flow among them. ©FUNPEC-RP. 2020-05-07T14:00:26Z 2020-05-07T14:00:26Z 2009 Artigo https://repositorio.inpa.gov.br/handle/1/15000 10.4238/vol8-3gmr624 en Volume 8, Número 3, Pags. 998-1007 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna, Mitochondrial Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase Subunit 5 Unclassified Drug Aedes Albopictus Asia Brasil Controlled Study Dna Determination Gene Structures Genetic Linkage Genetic Variability Haplotype Mutational Analysis Nonhuman Nucleotide Sequence Genetics, Population Population Structure Structure Analysis United States Aedes Animal Brasil Dna, Mitochondrial Gene Flow Genes, Insect Genes, Mitochondrial Genetic Variation Geography Haplotypes Aedes Albopictus Arbovirus Culicidae Diptera |
| spellingShingle |
Dna, Mitochondrial Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase Subunit 5 Unclassified Drug Aedes Albopictus Asia Brasil Controlled Study Dna Determination Gene Structures Genetic Linkage Genetic Variability Haplotype Mutational Analysis Nonhuman Nucleotide Sequence Genetics, Population Population Structure Structure Analysis United States Aedes Animal Brasil Dna, Mitochondrial Gene Flow Genes, Insect Genes, Mitochondrial Genetic Variation Geography Haplotypes Aedes Albopictus Arbovirus Culicidae Diptera Maia, R. T. Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene |
| topic_facet |
Dna, Mitochondrial Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase Reduced Nicotinamide Adenine Dinucleotide Dehydrogenase Subunit 5 Unclassified Drug Aedes Albopictus Asia Brasil Controlled Study Dna Determination Gene Structures Genetic Linkage Genetic Variability Haplotype Mutational Analysis Nonhuman Nucleotide Sequence Genetics, Population Population Structure Structure Analysis United States Aedes Animal Brasil Dna, Mitochondrial Gene Flow Genes, Insect Genes, Mitochondrial Genetic Variation Geography Haplotypes Aedes Albopictus Arbovirus Culicidae Diptera |
| description |
Aedes albopictus, a mosquito originally from Southeast Asia, is considered to be one of the main vectors of dengue fever, yellow fever and other arboviruses. We examined the genetic variability and population structure of 68 individuals of Ae. albopictus collected from five neighborhoods of the city of Manaus, based on the mitochondrial gene coding for NADH dehydrogenase subunit 5 (ND5). Two haplotypes were found, separated by a single mutational event (T ↔ C), with extremely low levels of genetic variability (h = 0.187 ± 0.059; π = 0.00044 ± 0.00014). Based on AMOVA, we concluded that most of the variation (99.08%) occurred within populations, though the levels of variation were not significant. Neutrality tests (Tajima's D and Fu's Fs) were non-significant, indicating that these populations are in genetic equilibrium. The most frequent haplotype (H1) is restricted to Brazilian populations of Ae. albopictus, while the rarer haplotype (H2) is shared with populations from the United States and Asia. We suggest that the reduced variability and low genetic structure identified in our study is a consequence of the recent introduction of this species in Manaus, possibly through a founder effect, followed by expansion throughout the city neighborhoods. Genetic similarity would therefore be due to insufficient time to have accumulated genetic differences between the populations of Ae. albopictus and not to extensive gene flow among them. ©FUNPEC-RP. |
| format |
Artigo |
| author |
Maia, R. T. |
| author2 |
Scarpassa, Vera Margarete Maciel-Litaiff, L. H. Tadei, Wanderli Pedro |
| author2Str |
Scarpassa, Vera Margarete Maciel-Litaiff, L. H. Tadei, Wanderli Pedro |
| title |
Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene |
| title_short |
Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene |
| title_full |
Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene |
| title_fullStr |
Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene |
| title_full_unstemmed |
Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene |
| title_sort |
reduced levels of genetic variation in aedes albopictus (diptera: culicidae) from manaus, amazonas state, brazil, based on analysis of the mitochondrial dna nd5 gene |
| publisher |
Genetics and Molecular Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15000 |
| _version_ |
1787143714438643712 |
| score |
11.653393 |