Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon.

Silva, C. A.; Lima, R. C.; Teixeira, A. S.

Artigo

Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon.

The discus is a very popular and expensive aquarium fish belonging to the family Cichlidae, genus Symphysodon, formed by three Amazon basin endemic species: Symphysodon aequifasciatus, S. discus and S. tarzoo. The taxonomic status of these fish is very controversial, with a paucity of molecular rese...

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Autor principal:	Silva, C. A.
Outros Autores:	Lima, R. C., Teixeira, A. S.
Grau:	Artigo
Idioma:	English
Publicado em:	Genetics and Molecular Research 2020
Assuntos:	Esterases Glucose-6-phosphate Isomerase Isoenzyme Lactate Dehydrogenase Malate Dehydrogenase Superoxide Dismutase Animals Brasil Classification Electrophoresis Enzymology Geography Metabolism Methodology Perciformes Muscle, Skeletal Species Difference Animal Brasil Electrophoresis Esterases Geography Glucose-6-phosphate Isomerase Isoenzymes L-lactate Dehydrogenase Malate Dehydrogenase Muscle, Skeletal Perciformes Species Specificity Superoxide Dismutase
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/15004

id	oai:repositorio:1-15004
recordtype	dspace
spelling	oai:repositorio:1-15004 Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon. Silva, C. A. Lima, R. C. Teixeira, A. S. Esterases Glucose-6-phosphate Isomerase Isoenzyme Lactate Dehydrogenase Malate Dehydrogenase Superoxide Dismutase Animals Brasil Classification Electrophoresis Enzymology Geography Metabolism Methodology Perciformes Muscle, Skeletal Species Difference Animal Brasil Electrophoresis Esterases Geography Glucose-6-phosphate Isomerase Isoenzymes L-lactate Dehydrogenase Malate Dehydrogenase Muscle, Skeletal Perciformes Species Specificity Superoxide Dismutase The discus is a very popular and expensive aquarium fish belonging to the family Cichlidae, genus Symphysodon, formed by three Amazon basin endemic species: Symphysodon aequifasciatus, S. discus and S. tarzoo. The taxonomic status of these fish is very controversial, with a paucity of molecular research on their population genetic structure and species identification. Information on molecular genetic markers, especially isoenzymes, in search of a better understanding of the population genetic structure and correct identification of fish species, has been receiving more attention when elaborating and implementing commercial fishery management programs. Aiming to contribute to a better understanding of the species taxonomic status, the present study describes the isoenzymatic patterns of 6 enzymes: esterase (Est - EC 3.1.1.1), lactate dehydrogenase (Ldh - EC 1.1.1.27), malate dehydrogenase (Mdh - EC 1.1.1.37), phosphoglucomutase (Pgm - EC 5.4.2.2), phosphoglucose isomerase (Pgi - EC 5.3.1.9), and super oxide dismutase (Sod - EC 1.15.1.1) extracted from skeletal muscle specimens and analyzed by starch gel electrophoresis. Monomorphic patterns, presumably controlled by 11 loci: Est-1, Est-2, Est-3, Ldh-1, Ldh-2, Mdh-1, Mdh-2, Pgi-1, Pgi-2, Pgm-1, and Sod-1 were fixed for the same alleles: Est-1(1), Est-2(1), Est-3(1), Ldh-1(1), Ldh-2(1), Mdh-1(1), Mdh-2(1), Pgi-1(1), Pgi-2(1), Pgm-1(1), and Sod-1(1), respectively, and detected in all 60 specimens examined (27 S. aequifasciatus from Manacapuru and 33 S. discus from Novo Airão, Central Amazon). The failure in the present study to detect diagnostic loci, which could be very useful for differentiating S. aequifasciatus from S. discus species, and polymorphic loci, which could also be applied for possible identification and delimitation of their stocks, does not rule out the possibility of there existing in other isoenzyme gene loci to be analyzed in the future. 2020-05-07T14:00:28Z 2020-05-07T14:00:28Z 2008 Artigo https://repositorio.inpa.gov.br/handle/1/15004 10.4238/vol7-3gmr450 en Volume 7, Número 3, Pags. 791-805 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Research
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Esterases Glucose-6-phosphate Isomerase Isoenzyme Lactate Dehydrogenase Malate Dehydrogenase Superoxide Dismutase Animals Brasil Classification Electrophoresis Enzymology Geography Metabolism Methodology Perciformes Muscle, Skeletal Species Difference Animal Brasil Electrophoresis Esterases Geography Glucose-6-phosphate Isomerase Isoenzymes L-lactate Dehydrogenase Malate Dehydrogenase Muscle, Skeletal Perciformes Species Specificity Superoxide Dismutase
spellingShingle	Esterases Glucose-6-phosphate Isomerase Isoenzyme Lactate Dehydrogenase Malate Dehydrogenase Superoxide Dismutase Animals Brasil Classification Electrophoresis Enzymology Geography Metabolism Methodology Perciformes Muscle, Skeletal Species Difference Animal Brasil Electrophoresis Esterases Geography Glucose-6-phosphate Isomerase Isoenzymes L-lactate Dehydrogenase Malate Dehydrogenase Muscle, Skeletal Perciformes Species Specificity Superoxide Dismutase Silva, C. A. Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon.
topic_facet	Esterases Glucose-6-phosphate Isomerase Isoenzyme Lactate Dehydrogenase Malate Dehydrogenase Superoxide Dismutase Animals Brasil Classification Electrophoresis Enzymology Geography Metabolism Methodology Perciformes Muscle, Skeletal Species Difference Animal Brasil Electrophoresis Esterases Geography Glucose-6-phosphate Isomerase Isoenzymes L-lactate Dehydrogenase Malate Dehydrogenase Muscle, Skeletal Perciformes Species Specificity Superoxide Dismutase
description	The discus is a very popular and expensive aquarium fish belonging to the family Cichlidae, genus Symphysodon, formed by three Amazon basin endemic species: Symphysodon aequifasciatus, S. discus and S. tarzoo. The taxonomic status of these fish is very controversial, with a paucity of molecular research on their population genetic structure and species identification. Information on molecular genetic markers, especially isoenzymes, in search of a better understanding of the population genetic structure and correct identification of fish species, has been receiving more attention when elaborating and implementing commercial fishery management programs. Aiming to contribute to a better understanding of the species taxonomic status, the present study describes the isoenzymatic patterns of 6 enzymes: esterase (Est - EC 3.1.1.1), lactate dehydrogenase (Ldh - EC 1.1.1.27), malate dehydrogenase (Mdh - EC 1.1.1.37), phosphoglucomutase (Pgm - EC 5.4.2.2), phosphoglucose isomerase (Pgi - EC 5.3.1.9), and super oxide dismutase (Sod - EC 1.15.1.1) extracted from skeletal muscle specimens and analyzed by starch gel electrophoresis. Monomorphic patterns, presumably controlled by 11 loci: Est-1, Est-2, Est-3, Ldh-1, Ldh-2, Mdh-1, Mdh-2, Pgi-1, Pgi-2, Pgm-1, and Sod-1 were fixed for the same alleles: Est-1(1), Est-2(1), Est-3(1), Ldh-1(1), Ldh-2(1), Mdh-1(1), Mdh-2(1), Pgi-1(1), Pgi-2(1), Pgm-1(1), and Sod-1(1), respectively, and detected in all 60 specimens examined (27 S. aequifasciatus from Manacapuru and 33 S. discus from Novo Airão, Central Amazon). The failure in the present study to detect diagnostic loci, which could be very useful for differentiating S. aequifasciatus from S. discus species, and polymorphic loci, which could also be applied for possible identification and delimitation of their stocks, does not rule out the possibility of there existing in other isoenzyme gene loci to be analyzed in the future.
format	Artigo
author	Silva, C. A.
author2	Lima, R. C. Teixeira, A. S.
author2Str	Lima, R. C. Teixeira, A. S.
title	Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon.
title_short	Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon.
title_full	Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon.
title_fullStr	Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon.
title_full_unstemmed	Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon.
title_sort	isoenzyme electrophoretic patterns in discus fish (symphysodon aequifasciatus pellegrin, 1904 and symphysodon discus heckel, 1840) from the central amazon.
publisher	Genetics and Molecular Research
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/15004
_version_	1787142934383034368
score	11.653393

Isoenzyme electrophoretic patterns in discus fish (Symphysodon aequifasciatus Pellegrin, 1904 and Symphysodon discus Heckel, 1840) from the Central Amazon.

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