Genetic diversity in populations of the viper Bothrops moojeni Hoge, 1966 in Central Brazil using RAPD markers
Bothrops moojeni is an abundant venomous snake responsible for most of the snakebite cases in the Central region of Brazil and as a result of the anthropogenic habitat disturbance, such as the increase in extensive farming, the range of B. moojeni has been greatly fragmented. Here, we obtained genom...
| Autor principal: | Dutra, Nicole C.L. |
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| Outros Autores: | Telles, Mariana Pires de Campos, Dutra, D. L., Silva, Nelson J. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15007 |
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oai:repositorio:1-15007 |
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oai:repositorio:1-15007 Genetic diversity in populations of the viper Bothrops moojeni Hoge, 1966 in Central Brazil using RAPD markers Dutra, Nicole C.L. Telles, Mariana Pires de Campos Dutra, D. L. Silva, Nelson J. Genomic Dna Brasil Controlled Study Genetic Conservation Genetic Variability Genome Analysis Nonhuman Random Amplified Polymorphic Dna Snake Bothrops Bothrops Moojeni Serpentes Viperinae Bothrops moojeni is an abundant venomous snake responsible for most of the snakebite cases in the Central region of Brazil and as a result of the anthropogenic habitat disturbance, such as the increase in extensive farming, the range of B. moojeni has been greatly fragmented. Here, we obtained genomic DNA from a total of 75 snakes belonging to four populations. Genetic variability evaluated for five RAPD primers was low (He = 0.20) and was not spatially structured. We found evidence of significant genetic divergence among B. moojeni populations that were isolated (ΦST values of 0.21 and 0.25), while populations more proximal exhibited less divergence (ΦST values of 0.04 and 0.08). We found only moderate divergence (ΦST value of 0.12) between two populations greatly isolated (851.83 km apart) along with great differentiation (0.24) between two proximal populations (290 km apart). Even though these populations are close to each other, they occur in an urbanized area that is almost completely covered by extensive crops, representing an obstruction to the mobility of this viper. Molecular variance analysis (AMOVA) showed some degree of subdivision in these populations, with a ΦST value of 0.16, significant to the level of 1% by 1000 random permutations. We also performed a Bayesian analysis that confirmed the AMOVA results and found a value of θB = 0.14 and an f = 0.27, suggesting a high level of endogamy. This is the first study that characterizes genetic variability for this important species of the Bothrops genus, and our data are of significant importance in terms of classifying populations in relation to their conservational value and management strategies. Thus, given the high levels of population structure found in this case, we recommend sampling as many populations as possible to maximize the genetic variability to be preserved when aiming for in situ conservation. The same should be done to perform samplings toward ex situ conservation. ©FUNPEC-RP. 2020-05-07T14:00:30Z 2020-05-07T14:00:30Z 2008 Artigo https://repositorio.inpa.gov.br/handle/1/15007 10.4238/vol7-3gmr413 en Volume 7, Número 3, Pags. 603-613 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Genomic Dna Brasil Controlled Study Genetic Conservation Genetic Variability Genome Analysis Nonhuman Random Amplified Polymorphic Dna Snake Bothrops Bothrops Moojeni Serpentes Viperinae |
| spellingShingle |
Genomic Dna Brasil Controlled Study Genetic Conservation Genetic Variability Genome Analysis Nonhuman Random Amplified Polymorphic Dna Snake Bothrops Bothrops Moojeni Serpentes Viperinae Dutra, Nicole C.L. Genetic diversity in populations of the viper Bothrops moojeni Hoge, 1966 in Central Brazil using RAPD markers |
| topic_facet |
Genomic Dna Brasil Controlled Study Genetic Conservation Genetic Variability Genome Analysis Nonhuman Random Amplified Polymorphic Dna Snake Bothrops Bothrops Moojeni Serpentes Viperinae |
| description |
Bothrops moojeni is an abundant venomous snake responsible for most of the snakebite cases in the Central region of Brazil and as a result of the anthropogenic habitat disturbance, such as the increase in extensive farming, the range of B. moojeni has been greatly fragmented. Here, we obtained genomic DNA from a total of 75 snakes belonging to four populations. Genetic variability evaluated for five RAPD primers was low (He = 0.20) and was not spatially structured. We found evidence of significant genetic divergence among B. moojeni populations that were isolated (ΦST values of 0.21 and 0.25), while populations more proximal exhibited less divergence (ΦST values of 0.04 and 0.08). We found only moderate divergence (ΦST value of 0.12) between two populations greatly isolated (851.83 km apart) along with great differentiation (0.24) between two proximal populations (290 km apart). Even though these populations are close to each other, they occur in an urbanized area that is almost completely covered by extensive crops, representing an obstruction to the mobility of this viper. Molecular variance analysis (AMOVA) showed some degree of subdivision in these populations, with a ΦST value of 0.16, significant to the level of 1% by 1000 random permutations. We also performed a Bayesian analysis that confirmed the AMOVA results and found a value of θB = 0.14 and an f = 0.27, suggesting a high level of endogamy. This is the first study that characterizes genetic variability for this important species of the Bothrops genus, and our data are of significant importance in terms of classifying populations in relation to their conservational value and management strategies. Thus, given the high levels of population structure found in this case, we recommend sampling as many populations as possible to maximize the genetic variability to be preserved when aiming for in situ conservation. The same should be done to perform samplings toward ex situ conservation. ©FUNPEC-RP. |
| format |
Artigo |
| author |
Dutra, Nicole C.L. |
| author2 |
Telles, Mariana Pires de Campos Dutra, D. L. Silva, Nelson J. |
| author2Str |
Telles, Mariana Pires de Campos Dutra, D. L. Silva, Nelson J. |
| title |
Genetic diversity in populations of the viper Bothrops moojeni Hoge, 1966 in Central Brazil using RAPD markers |
| title_short |
Genetic diversity in populations of the viper Bothrops moojeni Hoge, 1966 in Central Brazil using RAPD markers |
| title_full |
Genetic diversity in populations of the viper Bothrops moojeni Hoge, 1966 in Central Brazil using RAPD markers |
| title_fullStr |
Genetic diversity in populations of the viper Bothrops moojeni Hoge, 1966 in Central Brazil using RAPD markers |
| title_full_unstemmed |
Genetic diversity in populations of the viper Bothrops moojeni Hoge, 1966 in Central Brazil using RAPD markers |
| title_sort |
genetic diversity in populations of the viper bothrops moojeni hoge, 1966 in central brazil using rapd markers |
| publisher |
Genetics and Molecular Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15007 |
| _version_ |
1787144321658519552 |
| score |
11.755432 |