Differential expression of trypsin-3 and phosrestin ii genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon Region
Anopheles darlingi is the most anthropophilic mosquito related to Plasmodium infection of malaria, causing significant morbidity and mortality in South America. Pyrethroid chemical has been used to control mosquitos. We analyzed the expression of trypsin-3 and phosrestin II genes implicated to feedi...
| Autor principal: | Ohse, K. C. |
|---|---|
| Outros Autores: | Matiolli, Cleverson Carlos, Scarpassa, Vera Margarete, Santos, V. S., Tadei, Wanderli Pedro, Rafael, Míriam Silva |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15009 https://geneticsmr.com/sites/default/files/articles/year2017/vol16-4/pdf/differential-expression-of-trypsin3-and-phosrestin-ii-genes-in-the-main-malaria-vector-anopheles-darlingi-from-the-brazi.pdf |
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oai:repositorio:1-15009 Differential expression of trypsin-3 and phosrestin ii genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon Region Ohse, K. C. Matiolli, Cleverson Carlos Scarpassa, Vera Margarete Santos, V. S. Tadei, Wanderli Pedro Rafael, Míriam Silva Pyrethroid Adult Amazonas Anopheles Anopheles Albimanus Anopheles Darlingi Anopheles Gambiae Antenna (organ) Comparative Study Computer Model Controlled Study Female Gene Expression Gene Mutation Genetic Analysis Immune Response Insect Genetics Insect Larva Insecticide Resistance Mosquito Vector Mutation Rate Nonhuman Nucleotide Sequence Phosrestin Ii Gene Phylogenetic Tree Phylogeny Reverse Transcription Polymerase Chain Reaction Rna Sequence Signal Transduction Trypsin 3 Gene Anopheles darlingi is the most anthropophilic mosquito related to Plasmodium infection of malaria, causing significant morbidity and mortality in South America. Pyrethroid chemical has been used to control mosquitos. We analyzed the expression of trypsin-3 and phosrestin II genes implicated to feeding and resistance to insecticides, immune response and sensory antenna mechanisms, respectively, of larvae and adult of A. darlingi, through quantitative reverse transcription polymerase chain reaction (qRT-PCR). We aimed to validate the similarity in nucleotide sequences of A. darlingi RNA sequencing libraries by in silico, and qRT- PCR, owing to their possible effects on the ability to spread disease. The expression of trypsin-3 and phosrestin II was higher in the first and second instar larvae as compared with that in adults. These differentially expressed trypsin-3 and phosrestin II genes do not provide us evidence that both genes participate in pyrethroid resistance. The signaling pathway involving both genes requires further study. Preliminary phylogenetic relationships and the accumulation of mutations analysis in both genes were also compared with trypsin and phosrestin sequences of 15 and 17 other anopheline species, respectively, to obtain a mutational rate of 0.02 on phylogenetic trees. Trypsin gene of A. darlingi and A. albimanus clustered into the same group and was distinct from the species of A. gambiae complex and other anopheline. For phosrestin II, A. darlingi was separated from the remaining species from Africa, Asia, and Europe. Although the groups showed low to moderate support, it is possible to infer that both genes may belong to two evolutionary groups: one presents in the anopheline species of New World and other in the anopheline species of Old World, and be useful for future studies. © 2017 The Authors. 2020-05-07T14:00:31Z 2020-05-07T14:00:31Z 2017 Artigo https://repositorio.inpa.gov.br/handle/1/15009 https://geneticsmr.com/sites/default/files/articles/year2017/vol16-4/pdf/differential-expression-of-trypsin3-and-phosrestin-ii-genes-in-the-main-malaria-vector-anopheles-darlingi-from-the-brazi.pdf en Volume 16, Número 4 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Pyrethroid Adult Amazonas Anopheles Anopheles Albimanus Anopheles Darlingi Anopheles Gambiae Antenna (organ) Comparative Study Computer Model Controlled Study Female Gene Expression Gene Mutation Genetic Analysis Immune Response Insect Genetics Insect Larva Insecticide Resistance Mosquito Vector Mutation Rate Nonhuman Nucleotide Sequence Phosrestin Ii Gene Phylogenetic Tree Phylogeny Reverse Transcription Polymerase Chain Reaction Rna Sequence Signal Transduction Trypsin 3 Gene |
| spellingShingle |
Pyrethroid Adult Amazonas Anopheles Anopheles Albimanus Anopheles Darlingi Anopheles Gambiae Antenna (organ) Comparative Study Computer Model Controlled Study Female Gene Expression Gene Mutation Genetic Analysis Immune Response Insect Genetics Insect Larva Insecticide Resistance Mosquito Vector Mutation Rate Nonhuman Nucleotide Sequence Phosrestin Ii Gene Phylogenetic Tree Phylogeny Reverse Transcription Polymerase Chain Reaction Rna Sequence Signal Transduction Trypsin 3 Gene Ohse, K. C. Differential expression of trypsin-3 and phosrestin ii genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon Region |
| topic_facet |
Pyrethroid Adult Amazonas Anopheles Anopheles Albimanus Anopheles Darlingi Anopheles Gambiae Antenna (organ) Comparative Study Computer Model Controlled Study Female Gene Expression Gene Mutation Genetic Analysis Immune Response Insect Genetics Insect Larva Insecticide Resistance Mosquito Vector Mutation Rate Nonhuman Nucleotide Sequence Phosrestin Ii Gene Phylogenetic Tree Phylogeny Reverse Transcription Polymerase Chain Reaction Rna Sequence Signal Transduction Trypsin 3 Gene |
| description |
Anopheles darlingi is the most anthropophilic mosquito related to Plasmodium infection of malaria, causing significant morbidity and mortality in South America. Pyrethroid chemical has been used to control mosquitos. We analyzed the expression of trypsin-3 and phosrestin II genes implicated to feeding and resistance to insecticides, immune response and sensory antenna mechanisms, respectively, of larvae and adult of A. darlingi, through quantitative reverse transcription polymerase chain reaction (qRT-PCR). We aimed to validate the similarity in nucleotide sequences of A. darlingi RNA sequencing libraries by in silico, and qRT- PCR, owing to their possible effects on the ability to spread disease. The expression of trypsin-3 and phosrestin II was higher in the first and second instar larvae as compared with that in adults. These differentially expressed trypsin-3 and phosrestin II genes do not provide us evidence that both genes participate in pyrethroid resistance. The signaling pathway involving both genes requires further study. Preliminary phylogenetic relationships and the accumulation of mutations analysis in both genes were also compared with trypsin and phosrestin sequences of 15 and 17 other anopheline species, respectively, to obtain a mutational rate of 0.02 on phylogenetic trees. Trypsin gene of A. darlingi and A. albimanus clustered into the same group and was distinct from the species of A. gambiae complex and other anopheline. For phosrestin II, A. darlingi was separated from the remaining species from Africa, Asia, and Europe. Although the groups showed low to moderate support, it is possible to infer that both genes may belong to two evolutionary groups: one presents in the anopheline species of New World and other in the anopheline species of Old World, and be useful for future studies. © 2017 The Authors. |
| format |
Artigo |
| author |
Ohse, K. C. |
| author2 |
Matiolli, Cleverson Carlos Scarpassa, Vera Margarete Santos, V. S. Tadei, Wanderli Pedro Rafael, Míriam Silva |
| author2Str |
Matiolli, Cleverson Carlos Scarpassa, Vera Margarete Santos, V. S. Tadei, Wanderli Pedro Rafael, Míriam Silva |
| title |
Differential expression of trypsin-3 and phosrestin ii genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon Region |
| title_short |
Differential expression of trypsin-3 and phosrestin ii genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon Region |
| title_full |
Differential expression of trypsin-3 and phosrestin ii genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon Region |
| title_fullStr |
Differential expression of trypsin-3 and phosrestin ii genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon Region |
| title_full_unstemmed |
Differential expression of trypsin-3 and phosrestin ii genes in the main malaria vector, Anopheles darlingi, from the Brazilian Amazon Region |
| title_sort |
differential expression of trypsin-3 and phosrestin ii genes in the main malaria vector, anopheles darlingi, from the brazilian amazon region |
| publisher |
Genetics and Molecular Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15009 https://geneticsmr.com/sites/default/files/articles/year2017/vol16-4/pdf/differential-expression-of-trypsin3-and-phosrestin-ii-genes-in-the-main-malaria-vector-anopheles-darlingi-from-the-brazi.pdf |
| _version_ |
1787145064581955584 |
| score |
11.755432 |