Evidence for a natural hybrid of peacock bass (Cichla monoculus vs Cichla temensis) based on esterase electrophoretic patterns
Esterase (Est) and esterase-D (Est-D) electrophoretic patterns identified by starch gel electrophoresis of skeletal muscle protein extracts of 184 specimens of three species of peacock bass, locally known as tucunarés (Cichla monoculus, C. temensis and Cichla sp), plus four specimens of a supposed h...
| Autor principal: | Teixeira, Aylton Saturnino |
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| Outros Autores: | Oliveira, Suzana da Silva de |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetics and Molecular Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15012 https://www.geneticsmr.com/sites/default/files/articles/year2005/vol4-1/pdf/gmr0114.pdf |
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oai:repositorio:1-15012 |
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oai:repositorio:1-15012 Evidence for a natural hybrid of peacock bass (Cichla monoculus vs Cichla temensis) based on esterase electrophoretic patterns Teixeira, Aylton Saturnino Oliveira, Suzana da Silva de Esterases Muscle Protein Allele Cichla Monoculus Cichla Temensis Enzyme Activity Fish Gel Electrophoresis Gene Locus Nonhuman Organism Hybridization Muscle, Skeletal Animal Cichlids Electrophoresis, Starch Gel Esterases Hybridization, Genetic Muscle, Skeletal Bairdiella Ronchus Cichla Monoculus Cichla Temensis Esterase (Est) and esterase-D (Est-D) electrophoretic patterns identified by starch gel electrophoresis of skeletal muscle protein extracts of 184 specimens of three species of peacock bass, locally known as tucunarés (Cichla monoculus, C. temensis and Cichla sp), plus four specimens of a supposed hybrid (C. monoculus vs C. temensis), collected from the Central Amazon, were examined to determine if they could aid in identifying a supposed hybrid between C. monoculus and C. temensis. Six zones of electrophoretic activity were found with these enzyme systems. The Est enzyme showed one zone of activity, formed by bands 1, 2 and 3, plus three zones of activity, presumably controlled by Est-1, 2 and 3 loci. The Est-D enzyme had two zones of activity, presumably controlled by Est-D1 and Est-D2 loci. Cichla monoculus and C. temensis shared band 2 and alleles Est-11, Est-21, Est-32, and Est-D1 1, and therefore these were useless for identifying hybrids between the two species. However, a probable hybrid pattern of bands 1, 2, and 3, presumably generated by a combination of pattern 12 from C. monoculus with pattern 23 from C. temensis, resulting from a possible cross between these two species, was detected. Although the Est-D2 locus cannot be considered an ideal diagnostic marker for identifying the supposed hybrid (C. monoculus vs C. temensis), as it is polymorphic, it proved to be useful for determining the origin of the hybrid, i.e., which parental species were involved in the hybridization process. ©FUNPEC-RP. 2020-05-07T14:00:33Z 2020-05-07T14:00:33Z 2005 Artigo https://repositorio.inpa.gov.br/handle/1/15012 https://www.geneticsmr.com/sites/default/files/articles/year2005/vol4-1/pdf/gmr0114.pdf en Volume 4, Número 1, Pags. 74-83 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Genetics and Molecular Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Esterases Muscle Protein Allele Cichla Monoculus Cichla Temensis Enzyme Activity Fish Gel Electrophoresis Gene Locus Nonhuman Organism Hybridization Muscle, Skeletal Animal Cichlids Electrophoresis, Starch Gel Esterases Hybridization, Genetic Muscle, Skeletal Bairdiella Ronchus Cichla Monoculus Cichla Temensis |
| spellingShingle |
Esterases Muscle Protein Allele Cichla Monoculus Cichla Temensis Enzyme Activity Fish Gel Electrophoresis Gene Locus Nonhuman Organism Hybridization Muscle, Skeletal Animal Cichlids Electrophoresis, Starch Gel Esterases Hybridization, Genetic Muscle, Skeletal Bairdiella Ronchus Cichla Monoculus Cichla Temensis Teixeira, Aylton Saturnino Evidence for a natural hybrid of peacock bass (Cichla monoculus vs Cichla temensis) based on esterase electrophoretic patterns |
| topic_facet |
Esterases Muscle Protein Allele Cichla Monoculus Cichla Temensis Enzyme Activity Fish Gel Electrophoresis Gene Locus Nonhuman Organism Hybridization Muscle, Skeletal Animal Cichlids Electrophoresis, Starch Gel Esterases Hybridization, Genetic Muscle, Skeletal Bairdiella Ronchus Cichla Monoculus Cichla Temensis |
| description |
Esterase (Est) and esterase-D (Est-D) electrophoretic patterns identified by starch gel electrophoresis of skeletal muscle protein extracts of 184 specimens of three species of peacock bass, locally known as tucunarés (Cichla monoculus, C. temensis and Cichla sp), plus four specimens of a supposed hybrid (C. monoculus vs C. temensis), collected from the Central Amazon, were examined to determine if they could aid in identifying a supposed hybrid between C. monoculus and C. temensis. Six zones of electrophoretic activity were found with these enzyme systems. The Est enzyme showed one zone of activity, formed by bands 1, 2 and 3, plus three zones of activity, presumably controlled by Est-1, 2 and 3 loci. The Est-D enzyme had two zones of activity, presumably controlled by Est-D1 and Est-D2 loci. Cichla monoculus and C. temensis shared band 2 and alleles Est-11, Est-21, Est-32, and Est-D1 1, and therefore these were useless for identifying hybrids between the two species. However, a probable hybrid pattern of bands 1, 2, and 3, presumably generated by a combination of pattern 12 from C. monoculus with pattern 23 from C. temensis, resulting from a possible cross between these two species, was detected. Although the Est-D2 locus cannot be considered an ideal diagnostic marker for identifying the supposed hybrid (C. monoculus vs C. temensis), as it is polymorphic, it proved to be useful for determining the origin of the hybrid, i.e., which parental species were involved in the hybridization process. ©FUNPEC-RP. |
| format |
Artigo |
| author |
Teixeira, Aylton Saturnino |
| author2 |
Oliveira, Suzana da Silva de |
| author2Str |
Oliveira, Suzana da Silva de |
| title |
Evidence for a natural hybrid of peacock bass (Cichla monoculus vs Cichla temensis) based on esterase electrophoretic patterns |
| title_short |
Evidence for a natural hybrid of peacock bass (Cichla monoculus vs Cichla temensis) based on esterase electrophoretic patterns |
| title_full |
Evidence for a natural hybrid of peacock bass (Cichla monoculus vs Cichla temensis) based on esterase electrophoretic patterns |
| title_fullStr |
Evidence for a natural hybrid of peacock bass (Cichla monoculus vs Cichla temensis) based on esterase electrophoretic patterns |
| title_full_unstemmed |
Evidence for a natural hybrid of peacock bass (Cichla monoculus vs Cichla temensis) based on esterase electrophoretic patterns |
| title_sort |
evidence for a natural hybrid of peacock bass (cichla monoculus vs cichla temensis) based on esterase electrophoretic patterns |
| publisher |
Genetics and Molecular Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15012 https://www.geneticsmr.com/sites/default/files/articles/year2005/vol4-1/pdf/gmr0114.pdf |
| _version_ |
1787143170744647680 |
| score |
11.755432 |