Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure

Duarte, Rafael Mendonça; Smith, Donald Scott; Val, Adalberto Luis; Wood, Chris M.

Artigo

Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure

The so-called â œ blackwatersâ of the Amazonian Rio Negro are rich in highly coloured dissolved organic carbon (DOC), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. However these blackwaters support 8% of the worldâ ™ s ichthyofauna. We tested th...

ver descrição completa

Autor principal:	Duarte, Rafael Mendonça
Outros Autores:	Smith, Donald Scott, Val, Adalberto Luis, Wood, Chris M.
Grau:	Artigo
Idioma:	English
Publicado em:	Scientific Reports 2020
Assuntos:	Calcium Carbon Ion Sodium Animals Brasil Chemistry Growth, Development And Aging Ph Stress, Physiological River Zebrafish Animal Brasil Calcium Carbon Hydrogen-ion Concentration Ions Rivers Sodium Stress, Physiological Zebrafish
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/15225

id	oai:repositorio:1-15225
recordtype	dspace
spelling	oai:repositorio:1-15225 Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure Duarte, Rafael Mendonça Smith, Donald Scott Val, Adalberto Luis Wood, Chris M. Calcium Carbon Ion Sodium Animals Brasil Chemistry Growth, Development And Aging Ph Stress, Physiological River Zebrafish Animal Brasil Calcium Carbon Hydrogen-ion Concentration Ions Rivers Sodium Stress, Physiological Zebrafish The so-called â œ blackwatersâ of the Amazonian Rio Negro are rich in highly coloured dissolved organic carbon (DOC), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. However these blackwaters support 8% of the worldâ ™ s ichthyofauna. We tested the hypothesis that native DOC provides protection against ionoregulatory dysfunction in this extreme environment. DOCs were isolated by reverse-osmosis from two Rio Negro sites. Physico-chemical characterization clearly indicated a terrigenous origin, with a high proportion of hydroxyl and phenolic sites, high chemical reactivity to protons, and unusual proteinaceous fluorescence. When tested using zebrafish (a model organism), Rio Negro DOC provided almost perfect protection against ionoregulatory disturbances associated with acute exposure to pH 4.0 in ion-poor water. DOC reduced diffusive losses of Na + and Cl â', and promoted a remarkable stimulation of Na + uptake that otherwise would have been completely inhibited. Additionally, prior acclimation to DOC at neutral pH reduced rates of branchial Na + turnover, and provided similar protection against acid-induced ionoregulatory disturbances, even if the DOC was no longer present. These results reinforce the important roles that DOC molecules can play in the regulation of gill functions in freshwater fish, particularly in ion-poor, acidic blackwaters. 2020-05-07T14:14:50Z 2020-05-07T14:14:50Z 2016 Artigo https://repositorio.inpa.gov.br/handle/1/15225 10.1038/srep20377 en Volume 6 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Scientific Reports
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Calcium Carbon Ion Sodium Animals Brasil Chemistry Growth, Development And Aging Ph Stress, Physiological River Zebrafish Animal Brasil Calcium Carbon Hydrogen-ion Concentration Ions Rivers Sodium Stress, Physiological Zebrafish
spellingShingle	Calcium Carbon Ion Sodium Animals Brasil Chemistry Growth, Development And Aging Ph Stress, Physiological River Zebrafish Animal Brasil Calcium Carbon Hydrogen-ion Concentration Ions Rivers Sodium Stress, Physiological Zebrafish Duarte, Rafael Mendonça Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure
topic_facet	Calcium Carbon Ion Sodium Animals Brasil Chemistry Growth, Development And Aging Ph Stress, Physiological River Zebrafish Animal Brasil Calcium Carbon Hydrogen-ion Concentration Ions Rivers Sodium Stress, Physiological Zebrafish
description	The so-called â œ blackwatersâ of the Amazonian Rio Negro are rich in highly coloured dissolved organic carbon (DOC), but ion-poor and very acidic, conditions that would cause fatal ionoregulatory failure in most fish. However these blackwaters support 8% of the worldâ ™ s ichthyofauna. We tested the hypothesis that native DOC provides protection against ionoregulatory dysfunction in this extreme environment. DOCs were isolated by reverse-osmosis from two Rio Negro sites. Physico-chemical characterization clearly indicated a terrigenous origin, with a high proportion of hydroxyl and phenolic sites, high chemical reactivity to protons, and unusual proteinaceous fluorescence. When tested using zebrafish (a model organism), Rio Negro DOC provided almost perfect protection against ionoregulatory disturbances associated with acute exposure to pH 4.0 in ion-poor water. DOC reduced diffusive losses of Na + and Cl â', and promoted a remarkable stimulation of Na + uptake that otherwise would have been completely inhibited. Additionally, prior acclimation to DOC at neutral pH reduced rates of branchial Na + turnover, and provided similar protection against acid-induced ionoregulatory disturbances, even if the DOC was no longer present. These results reinforce the important roles that DOC molecules can play in the regulation of gill functions in freshwater fish, particularly in ion-poor, acidic blackwaters.
format	Artigo
author	Duarte, Rafael Mendonça
author2	Smith, Donald Scott Val, Adalberto Luis Wood, Chris M.
author2Str	Smith, Donald Scott Val, Adalberto Luis Wood, Chris M.
title	Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure
title_short	Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure
title_full	Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure
title_fullStr	Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure
title_full_unstemmed	Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure
title_sort	dissolved organic carbon from the upper rio negro protects zebrafish (danio rerio) against ionoregulatory disturbances caused by low ph exposure
publisher	Scientific Reports
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/15225
_version_	1787143914354900992
score	11.755432

Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure

Registros relacionados