Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil
Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya vir...
| Autor principal: | Aragão, Carine Fortes |
|---|---|
| Outros Autores: | Pinheiro, Valéria Cristina Soares, Neto, Joaquim Pinto Nunes, Silva, Eliana Vieira Pinto da, Pereira, Glennda Juscely Galvão, Nascimento, Bruna Laís Sena do, Castro, Karoline da Silva, Maia, Ariadne Mendonça, Catete, Clístenes Pamplona, Martins, Lívia Carício S., Tadei, Wanderli Pedro, Silva, Sandro Patroca da, Cruz, Ana Cecília Ribeiro |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Viruses
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15482 |
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oai:repositorio:1-15482 |
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oai:repositorio:1-15482 Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil Aragão, Carine Fortes Pinheiro, Valéria Cristina Soares Neto, Joaquim Pinto Nunes Silva, Eliana Vieira Pinto da Pereira, Glennda Juscely Galvão Nascimento, Bruna Laís Sena do Castro, Karoline da Silva Maia, Ariadne Mendonça Catete, Clístenes Pamplona Martins, Lívia Carício S. Tadei, Wanderli Pedro Silva, Sandro Patroca da Cruz, Ana Cecília Ribeiro Virus Rna Aedes Aegypti Aedes Albopictus Brasil Chikungunya Chikungunya Virus Controlled Study Dengue Dengue Virus 2 Female Microscopy, Fluorescence Gene Sequence Genotype Immunofluorescence Test Male Nonhuman Nucleotide Sequence Phylogenetic Tree Phylogeny Real-time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction Sequence Alignment Virus Isolation Virus Load Virus Transmission Zika Fever Zika Virus Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities-Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever. © 2019 by the authors. 2020-05-14T15:32:10Z 2020-05-14T15:32:10Z 2019 Artigo https://repositorio.inpa.gov.br/handle/1/15482 10.3390/v11121126 en Volume 11, Número 12 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Viruses |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Virus Rna Aedes Aegypti Aedes Albopictus Brasil Chikungunya Chikungunya Virus Controlled Study Dengue Dengue Virus 2 Female Microscopy, Fluorescence Gene Sequence Genotype Immunofluorescence Test Male Nonhuman Nucleotide Sequence Phylogenetic Tree Phylogeny Real-time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction Sequence Alignment Virus Isolation Virus Load Virus Transmission Zika Fever Zika Virus |
| spellingShingle |
Virus Rna Aedes Aegypti Aedes Albopictus Brasil Chikungunya Chikungunya Virus Controlled Study Dengue Dengue Virus 2 Female Microscopy, Fluorescence Gene Sequence Genotype Immunofluorescence Test Male Nonhuman Nucleotide Sequence Phylogenetic Tree Phylogeny Real-time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction Sequence Alignment Virus Isolation Virus Load Virus Transmission Zika Fever Zika Virus Aragão, Carine Fortes Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil |
| topic_facet |
Virus Rna Aedes Aegypti Aedes Albopictus Brasil Chikungunya Chikungunya Virus Controlled Study Dengue Dengue Virus 2 Female Microscopy, Fluorescence Gene Sequence Genotype Immunofluorescence Test Male Nonhuman Nucleotide Sequence Phylogenetic Tree Phylogeny Real-time Polymerase Chain Reaction Reverse Transcription Polymerase Chain Reaction Sequence Alignment Virus Isolation Virus Load Virus Transmission Zika Fever Zika Virus |
| description |
Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities-Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever. © 2019 by the authors. |
| format |
Artigo |
| author |
Aragão, Carine Fortes |
| author2 |
Pinheiro, Valéria Cristina Soares Neto, Joaquim Pinto Nunes Silva, Eliana Vieira Pinto da Pereira, Glennda Juscely Galvão Nascimento, Bruna Laís Sena do Castro, Karoline da Silva Maia, Ariadne Mendonça Catete, Clístenes Pamplona Martins, Lívia Carício S. Tadei, Wanderli Pedro Silva, Sandro Patroca da Cruz, Ana Cecília Ribeiro |
| author2Str |
Pinheiro, Valéria Cristina Soares Neto, Joaquim Pinto Nunes Silva, Eliana Vieira Pinto da Pereira, Glennda Juscely Galvão Nascimento, Bruna Laís Sena do Castro, Karoline da Silva Maia, Ariadne Mendonça Catete, Clístenes Pamplona Martins, Lívia Carício S. Tadei, Wanderli Pedro Silva, Sandro Patroca da Cruz, Ana Cecília Ribeiro |
| title |
Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil |
| title_short |
Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil |
| title_full |
Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil |
| title_fullStr |
Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil |
| title_full_unstemmed |
Natural infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a transition area of north-northeast Brazil |
| title_sort |
natural infection of aedes aegypti by chikungunya and dengue type 2 virus in a transition area of north-northeast brazil |
| publisher |
Viruses |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15482 |
| _version_ |
1787143173158469632 |
| score |
11.755432 |