Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart
Isoprene (C5H8) is a hydrocarbon gas emitted by many tree species and has been shown to protect photosynthesis under abiotic stress. Under optimal conditions for photosynthesis, ~70%-90% of carbon used for isoprene biosynthesis is produced fromrecently assimilated atmospheric CO2. While the contribu...
| Autor principal: | Garcia, Sabrina |
|---|---|
| Outros Autores: | Jardine, Kolby J., Souza, Vinícius Fernandes de, Souza, Rodrigo Augusto Ferreira de, Duvoisin Júnior, Sérgio, Gonçalves, José Francisco Carvalho de |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Forests
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15512 |
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oai:repositorio:1-15512 |
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oai:repositorio:1-15512 Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart Garcia, Sabrina Jardine, Kolby J. Souza, Vinícius Fernandes de Souza, Rodrigo Augusto Ferreira de Duvoisin Júnior, Sérgio Gonçalves, José Francisco Carvalho de Biochemistry Biosynthesis Carbon Carbon Dioxide Carboxylation Isoprene Photosynthesis Sodium Bicarbonate Abiotic Stress Carbon Source Decarboxylation Process Highest Temperature Photosynthesis Inhibitor Standard Conditions Temperature Dependent Tolerance Mechanisms Atmospheric Temperature Abiotic Factor Biogenic Emission Carbon Dioxide Decarboxylation Fruit Isoprene Leaf Photosynthesis Biochemistry Biosynthesis Carbon Dioxide Carboxylation Isoprene Inga Edulis Isoprene (C5H8) is a hydrocarbon gas emitted by many tree species and has been shown to protect photosynthesis under abiotic stress. Under optimal conditions for photosynthesis, ~70%-90% of carbon used for isoprene biosynthesis is produced fromrecently assimilated atmospheric CO2. While the contribution of alternative carbon sources that increase with leaf temperature and other stresses have been demonstrated, uncertainties remain regarding the biochemical source(s) of isoprene carbon. In this study, we investigated leaf isoprene emissions (Is) from neotropical species Inga edulis Mart. as a function of light and temperature under ambient (450 μmol m-2 s-1) and CO2-free (0 μmol m-2 s-1) atmosphere. Is under CO2-free atmosphere showed light-dependent emission patterns similar to those observed under ambient CO2, but with lower light saturation point. Leaves treated with the photosynthesis inhibitor DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) failed to produce detectable Is in normal light under a CO2-free atmosphere. While strong temperature-dependent Is were observed under CO2-free atmosphere in the light, dark conditions failed to produce detectable Is even at the highest temperatures studied (40 °C). Treatment of leaves with 13C-labeled sodium bicarbonate under CO2-free atmosphere resulted in Is with over 50% containing at least one 13C atom. Is under CO2-free atmosphere and standard conditions of light and leaf temperature represented 19% ± 7% of emissions under ambient CO2. The results show that the reassimilation of leaf internal CO2 contributes to Is in the neotropical species I. edulis. Through the consumption of excess photosynthetic energy, our results support a role of isoprene biosynthesis, together with photorespiration, as a key tolerance mechanism against high temperature and high light in the tropics. © 2019 by the authors. 2020-05-14T16:32:35Z 2020-05-14T16:32:35Z 2019 Artigo https://repositorio.inpa.gov.br/handle/1/15512 10.3390/f10060472 en Volume 10, Número 6 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Forests |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Biochemistry Biosynthesis Carbon Carbon Dioxide Carboxylation Isoprene Photosynthesis Sodium Bicarbonate Abiotic Stress Carbon Source Decarboxylation Process Highest Temperature Photosynthesis Inhibitor Standard Conditions Temperature Dependent Tolerance Mechanisms Atmospheric Temperature Abiotic Factor Biogenic Emission Carbon Dioxide Decarboxylation Fruit Isoprene Leaf Photosynthesis Biochemistry Biosynthesis Carbon Dioxide Carboxylation Isoprene Inga Edulis |
| spellingShingle |
Biochemistry Biosynthesis Carbon Carbon Dioxide Carboxylation Isoprene Photosynthesis Sodium Bicarbonate Abiotic Stress Carbon Source Decarboxylation Process Highest Temperature Photosynthesis Inhibitor Standard Conditions Temperature Dependent Tolerance Mechanisms Atmospheric Temperature Abiotic Factor Biogenic Emission Carbon Dioxide Decarboxylation Fruit Isoprene Leaf Photosynthesis Biochemistry Biosynthesis Carbon Dioxide Carboxylation Isoprene Inga Edulis Garcia, Sabrina Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
| topic_facet |
Biochemistry Biosynthesis Carbon Carbon Dioxide Carboxylation Isoprene Photosynthesis Sodium Bicarbonate Abiotic Stress Carbon Source Decarboxylation Process Highest Temperature Photosynthesis Inhibitor Standard Conditions Temperature Dependent Tolerance Mechanisms Atmospheric Temperature Abiotic Factor Biogenic Emission Carbon Dioxide Decarboxylation Fruit Isoprene Leaf Photosynthesis Biochemistry Biosynthesis Carbon Dioxide Carboxylation Isoprene Inga Edulis |
| description |
Isoprene (C5H8) is a hydrocarbon gas emitted by many tree species and has been shown to protect photosynthesis under abiotic stress. Under optimal conditions for photosynthesis, ~70%-90% of carbon used for isoprene biosynthesis is produced fromrecently assimilated atmospheric CO2. While the contribution of alternative carbon sources that increase with leaf temperature and other stresses have been demonstrated, uncertainties remain regarding the biochemical source(s) of isoprene carbon. In this study, we investigated leaf isoprene emissions (Is) from neotropical species Inga edulis Mart. as a function of light and temperature under ambient (450 μmol m-2 s-1) and CO2-free (0 μmol m-2 s-1) atmosphere. Is under CO2-free atmosphere showed light-dependent emission patterns similar to those observed under ambient CO2, but with lower light saturation point. Leaves treated with the photosynthesis inhibitor DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) failed to produce detectable Is in normal light under a CO2-free atmosphere. While strong temperature-dependent Is were observed under CO2-free atmosphere in the light, dark conditions failed to produce detectable Is even at the highest temperatures studied (40 °C). Treatment of leaves with 13C-labeled sodium bicarbonate under CO2-free atmosphere resulted in Is with over 50% containing at least one 13C atom. Is under CO2-free atmosphere and standard conditions of light and leaf temperature represented 19% ± 7% of emissions under ambient CO2. The results show that the reassimilation of leaf internal CO2 contributes to Is in the neotropical species I. edulis. Through the consumption of excess photosynthetic energy, our results support a role of isoprene biosynthesis, together with photorespiration, as a key tolerance mechanism against high temperature and high light in the tropics. © 2019 by the authors. |
| format |
Artigo |
| author |
Garcia, Sabrina |
| author2 |
Jardine, Kolby J. Souza, Vinícius Fernandes de Souza, Rodrigo Augusto Ferreira de Duvoisin Júnior, Sérgio Gonçalves, José Francisco Carvalho de |
| author2Str |
Jardine, Kolby J. Souza, Vinícius Fernandes de Souza, Rodrigo Augusto Ferreira de Duvoisin Júnior, Sérgio Gonçalves, José Francisco Carvalho de |
| title |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
| title_short |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
| title_full |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
| title_fullStr |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
| title_full_unstemmed |
Reassimilation of leaf internal CO2 contributes to isoprene emission in the neotropical species inga edulis Mart |
| title_sort |
reassimilation of leaf internal co2 contributes to isoprene emission in the neotropical species inga edulis mart |
| publisher |
Forests |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15512 |
| _version_ |
1787145417012543488 |
| score |
11.755432 |